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ARTIGOS ACEITOS
PARA PUBLICAÇÃO
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A001-99 Ha-ras
oncogene effect on DNA content and chromatin supraorganization
in benzo[a]pyrene-transformed human breast epithelial cells
Mello MLS*, Vidal
BC*, Russo J
When transfected
to benzo[a]pyrene (BP)-transformed MCF-10F human breast epithelial
cells (BP1 cell line) the c-Ha-ras oncogene has proven
to enhance the neoplastic changes initiated by exposure to
BP, giving rise to an aggressive tumorigenic cell line, BP1-Tras.
We have previously demonstrated that BP affects the DNA content
and the chromatin supraorganization of MCF-10F cells. Here,
Feulgen-stained BP1-Tras cells were studied by image
analysis in order to evaluate possible additional changes
in DNA content and chromatin texture induced by insertion
of the ras oncogene. A high variability in DNA content,
and an increase in the packing states of the chromatin which
became still condensed in BP1 cells were found in BP1-Tras
cells. The results differed from those reported for the BP1-E1
cell line which is also an aggressive tumorigenic cell line,
but was attained through progressive passages of BP-transformed
cells. It was demonstrated that different patterns of changes
in DNA content and chromatin organization may be involved
in equally aggressive tumorigenic BP-transformed cell lines
originated from the same cell line by different mechanisms.
Analytical Cellular Pathology – Accepted on June,
1999
*E-mail: mlsmello@unicamp.br;
vidal@unicamp.br
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A002-99 Atividades
reprodutivas e desenvolvimento de Camptischium clavipes
(Fabricius, 1803) (Hemiptera, Coreidae) sob condições de laboratório
Amaral Filho
BF*
Revista Brasileira
de Entomologia 43(3/4)
*E-mail: morgamar@bestway.com.br
A003-99 Nuclear
phenotype changes after heat shock in Panstrongylus megistus
(Burmeister)
Garcia SL, Mello
MLS*, Rodrigues VLCC, Garcia NL
The nuclear phenotypes
of Malpighian tubule epithelial cells of male nymphs of P.
megistus subjected to short- and long-duration heat shocks
at 40oC were analyzed immediately after the shock
and 10 and 30 days later. The frequency of the various nuclear
phenotypes observed suggests that the forms of cell survival
observed were not sufficient or efficient enough to protect
all of the Malpighian tubule cells from the deleterious effects
of stress. In agreement with studies on P. megistus
survival following heat shock, only long-duration shock produced
strongly deleterious effects. Memórias do Instituto Oswaldo
Cruz – Accepted on November, 1999
*E-mail: mlsmello@unicamp.br
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A004-99 Image
analysis of DNA fragmentation and loss in V79 cells under
apoptosis
Maria SS, Vidal
BC*, Mello MLS*
Nuclear image analysis
of Feulgen-stained V79 fibroblasts after 3 days in culture
was used to discriminate apoptotic cells and cells suspected
to be undergoing apoptosis from control cells, based on parameters
such as the Feulgen-DNA content, degree of chromatin condensation
and nuclear areas in association with visual morphology. Three
nuclear phenotypes were defined in terms of their morphological
characteristics and levels of chromatin condensation. A decrease
in the amount of Feulgen-DNA assumed to indicate DNA loss
was detected in apoptotic cells and in cells suspected of
undergoing apoptosis. Image analysis procedures may therefore
provide a useful tool for discriminating cells even in the
early stages of apoptosis.Genetics and Molecular Biology
– Accepted on September, 1999
E-mail: vidal@unicamp.br;
mlsmello@unicamp.br
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A005-99 Adaptative
response to swim exercise: antioxidant system and lipid peroxidation
Cesquine M,Torsoni
MA, Ogo SH*
(JAAM) American
Anti-aging Medicine
*E-mail: secrbioq@unicamp.br
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A006-99 Purification
and kinetic properties of a castor bean seed acid phosphatase
containing sulfhydryl groups
Granjeiro PA,
Ferreira CV, Granjeiro JM, Taga EM, Aoyama H*
Acid phosphatase
was purified from Ricinus communis seeds to homogeneity,
with a specific activity of 3.8 µkat mg-1, and
a relative molecular mass of 60 kDa presented a pH optimum
of 5.5 and an apparent Km value for p-nitrophenylphosphate
of 0.52 mM. The strong inhibition of the enzyme by pCMB,
Cu2+ and vanadate suggest the presence of sulfhydryl
groups essential for catalysis. The enzyme also recognizes
tyrosine-phosphate and inorganic pyrophosphate (PPi) as substrate.
Based on the highest specificity constant (Vmax/Km),
PPi could be a potencial physiological substrate. Physiologia
Plantarum – Accepted on July, 1999
*E-mail: aoyama@unicamp.br
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A007-99 Inhibition
of acid phosphatase isoforms purified from mature soybean
(Glycine max) seeds
Ferreira CV,
Taga EM, Aoyama H*
Four soybean seed
acid phosphatase isoforms (API, AP2, AP3A and AP3B) were competitively
inhibited by phosphate, vanadate, fluoride and molybdate,
using p-nitrophenylphosphate as substrate but were not significantly
affected neither by compounds that can interact with -SH residues
nor by pyridoxal phosphate, suggesting that cysteine and lysine
residues are not present in the active site. Journal of
Enzyme Inhibition – Accepted on September, 1999
E-mail: aoyama@unicamp.br
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A008-99 Chromatin
organization in normal and cancer cells
Mello MLS*, Vidal
BC*
A brief overview
on basic concepts about chromatin organization is presented
with special reference to changes promoted in transformed
and tumorigenic cells and to some unsolved questions as regards
morphological images vs. chromatin physiology. J. Women’s
Cancer 2, in press
*E-mail: mlsmello@unicamp.br,
vidal@unicamp.br
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A009-99 Biological
activities of violacein a new antitumoral indole derivative
in na inclusion complex with Beta-cyclodextrins
Azevedo MBM,
Alderete J, Rodrigues JA, Souza AO, Rettori D, Torsoni MA,
Faljoni-Alario A, Haun M*, Duran N
Journal of Inclusion Phenomena, 1999
*E-mail: secrbioq@unicamp.br
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