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Instituto de Biologia Unicamp






ABSTRACTA IB (2) - 1999


A001-99 Ha-ras oncogene effect on DNA content and chromatin supraorganization in benzo[a]pyrene-transformed human breast epithelial cells

Mello MLS*, Vidal BC*, Russo J

When transfected to benzo[a]pyrene (BP)-transformed MCF-10F human breast epithelial cells (BP1 cell line) the c-Ha-ras oncogene has proven to enhance the neoplastic changes initiated by exposure to BP, giving rise to an aggressive tumorigenic cell line, BP1-Tras. We have previously demonstrated that BP affects the DNA content and the chromatin supraorganization of MCF-10F cells. Here, Feulgen-stained BP1-Tras cells were studied by image analysis in order to evaluate possible additional changes in DNA content and chromatin texture induced by insertion of the ras oncogene. A high variability in DNA content, and an increase in the packing states of the chromatin which became still condensed in BP1 cells were found in BP1-Tras cells. The results differed from those reported for the BP1-E1 cell line which is also an aggressive tumorigenic cell line, but was attained through progressive passages of BP-transformed cells. It was demonstrated that different patterns of changes in DNA content and chromatin organization may be involved in equally aggressive tumorigenic BP-transformed cell lines originated from the same cell line by different mechanisms. Analytical Cellular Pathology – Accepted on June, 1999

A002-99 Atividades reprodutivas e desenvolvimento de Camptischium clavipes (Fabricius, 1803) (Hemiptera, Coreidae) sob condições de laboratório

Amaral Filho BF*

Revista Brasileira de Entomologia 43(3/4)

A003-99 Nuclear phenotype changes after heat shock in Panstrongylus megistus (Burmeister)

Garcia SL, Mello MLS*, Rodrigues VLCC, Garcia NL

The nuclear phenotypes of Malpighian tubule epithelial cells of male nymphs of P. megistus subjected to short- and long-duration heat shocks at 40oC were analyzed immediately after the shock and 10 and 30 days later. The frequency of the various nuclear phenotypes observed suggests that the forms of cell survival observed were not sufficient or efficient enough to protect all of the Malpighian tubule cells from the deleterious effects of stress. In agreement with studies on P. megistus survival following heat shock, only long-duration shock produced strongly deleterious effects. Memórias do Instituto Oswaldo Cruz – Accepted on November, 1999

A004-99 Image analysis of DNA fragmentation and loss in V79 cells under apoptosis

Maria SS, Vidal BC*, Mello MLS*

Nuclear image analysis of Feulgen-stained V79 fibroblasts after 3 days in culture was used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells, based on parameters such as the Feulgen-DNA content, degree of chromatin condensation and nuclear areas in association with visual morphology. Three nuclear phenotypes were defined in terms of their morphological characteristics and levels of chromatin condensation. A decrease in the amount of Feulgen-DNA assumed to indicate DNA loss was detected in apoptotic cells and in cells suspected of undergoing apoptosis. Image analysis procedures may therefore provide a useful tool for discriminating cells even in the early stages of apoptosis.Genetics and Molecular Biology – Accepted on September, 1999

A005-99 Adaptative response to swim exercise: antioxidant system and lipid peroxidation

Cesquine M,Torsoni MA, Ogo SH*

(JAAM) American Anti-aging Medicine

A006-99 Purification and kinetic properties of a castor bean seed acid phosphatase containing sulfhydryl groups

Granjeiro PA, Ferreira CV, Granjeiro JM, Taga EM, Aoyama H*

Acid phosphatase was purified from Ricinus communis seeds to homogeneity, with a specific activity of 3.8 µkat mg-1, and a relative molecular mass of 60 kDa presented a pH optimum of 5.5 and an apparent Km value for p-nitrophenylphosphate of 0.52 mM. The strong inhibition of the enzyme by pCMB, Cu2+ and vanadate suggest the presence of sulfhydryl groups essential for catalysis. The enzyme also recognizes tyrosine-phosphate and inorganic pyrophosphate (PPi) as substrate. Based on the highest specificity constant (Vmax/Km), PPi could be a potencial physiological substrate. Physiologia Plantarum – Accepted on July, 1999

A007-99 Inhibition of acid phosphatase isoforms purified from mature soybean (Glycine max) seeds

Ferreira CV, Taga EM, Aoyama H*

Four soybean seed acid phosphatase isoforms (API, AP2, AP3A and AP3B) were competitively inhibited by phosphate, vanadate, fluoride and molybdate, using p-nitrophenylphosphate as substrate but were not significantly affected neither by compounds that can interact with -SH residues nor by pyridoxal phosphate, suggesting that cysteine and lysine residues are not present in the active site. Journal of Enzyme Inhibition – Accepted on September, 1999

A008-99 Chromatin organization in normal and cancer cells

Mello MLS*, Vidal BC*

A brief overview on basic concepts about chromatin organization is presented with special reference to changes promoted in transformed and tumorigenic cells and to some unsolved questions as regards morphological images vs. chromatin physiology. J. Women’s Cancer 2, in press


A009-99 Biological activities of violacein a new antitumoral indole derivative in na inclusion complex with Beta-cyclodextrins

Azevedo MBM, Alderete J, Rodrigues JA, Souza AO, Rettori D, Torsoni MA, Faljoni-Alario A, Haun M*, Duran N
Journal of Inclusion Phenomena, 1999


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