ARTIGOS PUBLICADOS EM PERIÓDICOS

P050-99 Effect of Ca2+ channel blockers on arterial hypertension and heart ischaemic lesions induced by chronic blockade of nitric oxide in the rat de Oliveira CF, Nathan LP, Metze K, Moreno H Jr, De Luca IMS*, Sucupira M, Zats R, Zappellini A, Antunes E, De Nucci G The effects of the Ca2+ channel blockers diltiazem, nifedipine and amlodipine were studied on both arterial hypertension and myocardial changes induced by chronic blockade of nitric oxide synthesis. Control male Wistar rats received Nw -nitro-L-arginine methyl ester (L-NAME, 20 mg rat/day) in the drinking water for 8 weeks; blood pressure and body weight were monitored weekly. Ca2+ channel blockers were given concomitantly to L-NAME. L-NAME induced a time dependent increase in blood pressure which was significantly attenuated by diltiazem, nifedipine and amlodipine. Rats treated with the L-NAME also developed myocardial ischaemia. Neither diltiazem nor nifedipidine prevented this effect whereas amlodipine virtually abolished the increase in fibrous tissue induced by L-NAME. The plasma concentration of the Ca2+ channels blockers was measured by liquid chromatography coupled to mass spectofotometry at two different time points (morning and afternoon). Only amlodipine treatment was able to maintain constant levels in the morning and in the evening.. We conclude that amlodipine (but not diltiazem and nifedipine) can efficiently control myocardial ischaemia in nitric oxide deficient rats, probably due to its intrinsically long half-life. European Journal of Pharmacology 373 (2-3): 195-200, 1999. IF= 1.960 *E-mail: ideluca@unicamp.br

051-99 Influence of complete spinal cord injury on skeletal muscle within 6 mo of injury Castro MJ, Apple DF, Staron RS, Campos GER*, Dudley GA The influence of spinal cord injury (SCI) on affected skeletal muscle was studied. The right vastus lateralis muscle was biopsied in 12 patientes as soon as they were clinically stable (average 6 wk after SCI), and 11 and 24 wk after injury. Nine able-bodied controls were used at two time points 18 wk apart. Surface electrical stimulation (ES) was applied to the left quadriceps femoris muscle to assess fatigue at these same time intervals. Biopsies were analyzed for fiber type percent and cross-sectional area (CSA), fiber type-specific SDH and GPDH activities, and myosin heavy chain percent. Controls showed no change in any variable over time. The results indicate that vastus lateralis muscle shows marked fiber atrophy, no change in the proportion of type I fibers, and a relative independence of metabolic enzyme levels from activation during the first 24 wk after clinically complete SCI. Over this time, quadriceps femoris muscle showed moderately greater force loss during ES in patients than in controls. It is suggested that the predominant response of mixed human skeletal muscle within 6 mo of SCI is loss of contractile protein. Therapeutic interventions could take advantage of this to increase muscle mass. Journal of Applied Physiology 86: 350-358, 1999. IF= 1.630 *E-mail: grcampos@obelix.unicamp.br

052-99 Fiber type composition of four hindlimb muscles of adult Fisher 344 rats Staron RS, Kraemer WJ, Hikida RS, Fry AC, Murray JD, Campos GER* The limb and trunk muscles of adult rats express four myosin heavy chain (MHC) isoforms, one slow (MHCI) and three fast (MHCIIa, MHCIId, and MHCIIb). The distribution of these isoforms correlates with fiber types delineated using mATPase histochemistry. Fibers containing only one MHC isoform have been termed "pure" fibers. Recent evidence suggests that a population of "hybrid" fibers exist in rat skeletal muscle which contain two MHC isoforms. The purpose of the present investigation was to document the entire range of histochemically defined "pure" and "hybrid" fiber types in untreated muscles of the young adult Fisher 344 rat hindlimb. The selected hindlimb muscles were removed from 12 male rats and analyzed for muscle fiber type distribution, cross-sectional area, and MHC content. Care was taken to delineate eight fiber types using refined histochemical techniques. Hybrid fibers were found to make up a considerable portion of the muscles examined. The deep red portion of the gastrocnemius muscle contained the largest number of hybrid fibers, most of which were the fast types IIAD and IIDB. In conclusion, hybrid fibers make up a considerable portion of normal rat limb musculature and are an important population that should not be ignored. Histochemistry and Cell Biology 111: 117-123, 1999. IF= 1.730 *E-mail: grcampos@obelix.unicamp.br

P053-99 Ultrastructural changes on the epithelial cells of uterine tubes of wistar rats after chronic ethanol ingestion Martinez M, Branco Jr AC, Cagnon VHA*, Mello Júnior W, Garcia PJ, Martinez FE Morphological changes in the epithelial layer of the uterine tubes of rats submitted to experimental chronic alcoholism were studied with anatomical, histological, ultrastructural and morphometric methods. Sixty adult rats at the same age (3 months) and with a mean body weight of 228 g were divided into two groups. The control group received solid Purina rat chow diet and tap water ad libitum. The alcoholic group received the same solid diet and was allowed to drink only sugar cane brandy dissolved in 30° Gay Lussac (v/v). After periods of 90, 180 and 270 days materials of treated animals at normal estrus were analyzed. The final mean body weights were similar in the control and alcoholic groups. The morphometric analysis revealed no difference between control and alcoholic epithelial height. Ultrastructural alterations like intense lipid droplet and lysosomes accumulation, dilated rough endoplasmic reticulum cisternae and vaacuolization were found in the alcoholic rats after both periods of treatment. Alcohol thus acts as a toxin on the epithelial layer of the uterine tubes of rats. Journal of Submicroscopic Cytology and Pathology 31: 273-278, 1999. IF= 0.690 *E-mail: quitete@uol.com.br

P054-99 Porous Polly (L-lactide) membranes obtained by immersion precipitation process: morphology, phase separation an culture of vero cells Zoppi RA, Constant S, Duek EAR, Marques FR, Wada MLF*, Nunes SP The objective was obtaining porous membranes of Polly lactic acid (PPLA) with the method of phase inversion ,varying the concentration of the polymer, or the solvent. SEM observations showed that low polymer concentrations (1.8%) resulted in membranes with many small pores. Above 3% the membrane was dense. Ethanol, methanol or DMF had no influence in pore size. The membranes obtained with 3 and 4 % polymer concentration did not interfere with the vero cell growth pattern. Membranes formed by phase inversion do not support cell growth in their porosities but only on their external surface. Polymer 40: 3275-3289, 1999. IF= 1.358 *E-mail:wada@obelix.unicamp.br

P055-99 Alterations of chromosome number in cultured cells induced by dexametasone Carvalho CE, Santos Jr AR, Wada MLF* Dexamethasone (DEX), a synthetic glucocorticoid, has been used as a general induce of cell differentiation. We analyzed the chromosome number of three cells: human lymphocytes, a diploid cell lineage (V-79) and an aneuploid cell lineage (Vero) treated with DEX. The poliploid index reached 13.4 % in Vero cells (control, 5.6%) and 11.4 % in V-79 cells (control, 1%) treated with DEX. We did not find any alterations in chromosome number in lymphocytes following DEX treatment. DEX may act differently in lymphocytes as compared with established cell lineage where it induces chromosome number alterations. Medical Science Research 27: 219-221, 1999. IF= 0.367 *E-mail: wada@obelix.unicamp.br

P056-99 Foetal calf serum and dexamethasone effects on vero cells. Growth and differentiation Santos Jr AR, Wada MLF* Vero cells were cultured without fetal calf serum (FCS), with 10% FCS, 10% FCS plus dexamethasone (DEX) or 20% FCS for 48, 120 or 240hs. The cells were analyzed by a growth curve, cytochemical and immunocytochemical methods. In 48hs vero cells produced fibronectin and collagen IV. The growth of metachromatic multicellular masses was induced by DEX. The vero cells produced collagen IV with 10 and 20% FCS, and also cells wich did not have this activity. The multicellular masses induced by DEX were rich in fibronectin. DEX induced differentiation and the expression of collagen IV and fibronectin in Vero cells. Cytobios 99:159-199, 1999 *E-mail: wada@obelix.unicamp.br

P057-99 Glandular trichomes on the leaves and flowers of Plectranthus ornatus: morphology, distribution and histochemistry Ascensão L, Mota L, Castro MM* The types of glandular trichomes and their distribution on leaves and flowers of Plectranthus ornatus were investigated at different stages of their development. Five morphological types of glandular trichomes are described. The reproductive organs, particularly the calyx and corolla, exhibit, in addition to the reported trichomes, unusual conoidal trichomes with long unicellular conical heads. A large apical pore, formed by tip disruption, releases the secretion (a gumresin) stored in a rostrum-like projection. On the stamens and capels, digitiform, capitate and conoidal trichomes are absent, but peltate trichomes are numerous. The results presented are compared with those of other studies on Lamiaceae glandular trichomes. Annals of Botany 84:437-447, 1999. IF= 1.301 *E-mail: marilac@unicamp.br

P058-99 Adenocalymma ubatubensis. Assis & Semir, a new species of Bignoniaceae from Ubatuba, São Paulo state Brazil Assis MA, Semir J* Adenocalymma ubatubensis, a new species from southeastern coastal Brazil, is described and illustrated. The species is ditinguished by its large pseudostipuls and calyx wiht-out nectaries. Novon 9: 136-138, 1999 *E-mail: semir@unicamp.br

P059-99 Temporal variation in pollinarium size after its removal in species of Bulbophyllum: a different mechanism preventing self pollination in Orchidaceae Borba EL, Semir J* In Bulbophyllum involutum and B. ipanemense (Orchidaceae), two closely related species, shortly after removal the pollinarium has a diameter of approximately twice that of the entrance of the stigmatic cavity, requiring a mean time of 105-135 min to shrink in width and allow pollination. Because the pollinators of these species remain for some min in the same flower after removing the pollinia this mechanism, previously unknown in Orchidaceae, may be very important in preventing self-pollination. This mechanism does not occur in B. weddelii. Plant, Systematics and Evolution 217: 197-204, 1999. IF= 0.824 *E-mail: semir@unicamp.br

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