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Instituto de Biologia Unicamp






ABSTRACTA IB (2) - 1999


P100-99 Constância, Dominância e Freqüência Mensal de dípteros muscóides e seus parasitóides (Hymenoptera e Coleoptera) associados a fezes frescas de gado bovino em Uberlândia, MG

Marchiori CH, Linhares AX*

Mensalmente, 10 amostras de esterco bovino com aproximadamente uma semana de idade foram colhidas aleatoriamente em pastagens, transportadas para o laboratório, e postas em baldes com água. Os artrópodes eram extraídos dessas amostras cinco dias após a coleta no campo. As pupas eram retiradas com peneira e acondicionadas individualmente em cápsulas de gelatina até sua emergência ou a de seus parasitóides. Neste sítio, 50% dos hospedeiros predominaram na estação quente e úmida; os parasitóides predominaram na estação fria e seca. As espécies de Palaeosepsis insularis Williston e Eucoilidae sp.3 foram dominantes.Anais da Sociedade Entomológica do Brasil 28: 375-387, 1999.

P101-99 Primeira ocorrência de Neralsia splendens (Borgmeier) (Hymenoptera: Figitidae) na região do Triângulo Mineiro e Sul de Goiás

Marchiori C, Linhares AX*
Anais da Sociedade Entomológica do Brasil 28: 543-544, 1999 (Nota científica).

P102-99 Description of Pythonella scleruri n.sp. (Apicomplexa: Eimeriidae) from a Brazilian Bird Rufous-Breasted-Leaftosser Sclerurus scansor, 1835 (Passerifirmes: Furnariidae)

Kawazoe U*, Gouvea H

Coccidian oocysts containing 16 sporocysts with 4 sporozoites in each were observed in a faecal sample from Sclerurus scansor collected in a the Itatiaia National Park, southeastern region of Brazil. The oocysts are characterized by ellipsoidal shape measuring 42.5 x 32.8 m m, with smooth, thick double-layered wall of a greenish-orange colour. The presently described coccidian, recorded for the first time in birds, is a new species named Pythonella scleruri. Memórias do Instituto Oswaldo Cruz 94: 157-159, 1999. IF= 0.440

P103-99 Role of the hippocampus in contextual memory after classical aversive conditioning in pigeons (C. livia)

Reis F, Schenka AA, Melo LL, Ferrari EAM*

We investigated the effects of hippocampal lesions with ibotenic acid (IBO) on the memory of the sound-context-shock association analyzed during reexposure to the context of conditioning. Twenty-nine adult male pigeons were assigned to a non-lesioned group (CG), a sham-lesioned group (SG) and a hippocampus-lesioned group (EG), and to a non-paired non-lesioned animal group (tone-alone exposure) (NG). All pigeons were submitted to a 20 min session in the conditioning chamber with three associations of sound and shock. Experimental and sham lesions were performed 24 hours later (SG and EG) when EG birds received three bilateral injections of IBO and SG received one bilateral injection of PBS. Reexposure to the training context ocurred 5 days after the lesion. Behavior was videotaped for 20 min and analyzed at 30 sec intervals. A significantly higher occurrence of freezing was observed for CG and SG compared to EG and NG groups in the training context. These results suggest impairment of contextual fear in birds that received lesions one day after conditioning and a hippocampal role in the modulation of emotional aversive memories in pigeons.Brazilian Journal of Medical and Biological Research 32: 1127-1131, 1999. IF=0.468

P104-99 Fluorescence and confocal laser scanning microscopy of H&E stained sections for the study of elastic fibers in skin and in some skin disorders

Carvalho HF*, Taboga SR, Felisbino SL

We have previously suggested that examination of H&E stained sections by fluorescence microscopy allows for good imaging of elastic fiber structure and distribution. Since these parameters are important for the characterization of elastic fibers and for diagnosing some skin diseases, we show in this paper that dermal elastic fibers are consistently imaged by H&E plus fluorescence microscopy, by the analysis of normal and diseased skin samples, and that the use of confocal laser scanning microscopy greatly increases the image quality, which in turn will permit image analysis to be carried out. The proposed procedure revealed to be a revelant tool in elastic fiber research and the present technique is of interest for a large group of investigators using H&E as a routine method.Brazilian Journal of Morphological Science 16: 97-104, 1999.

P105-99 Development of the pressure-bearing tendon of the bullfrog, Rana catesbeiana

Carvalho HF*, Felisbino SL

The plantaris longus tendon of the bullfrog is a pressure-bearing tendon and develops a fibrocartilage-like arrangement in the area subjected to compressive forces. The fibrocartilage-like tissue shows some distinct aspects of cellular and fibrillar structure and distribution when compared to the mammalian counterparts. Development of the plantaris longus tendon was assessed in developing tadpoles by investigating some of its structural, cytochemical and immunocytochemical aspects. The results indicated that the compression region does not arise by simple hyperplasia of the peripheral layers of a normal tendon, but from a programmed sequence of developmental steps. It is also suggested that the mechanical stimulation is a secondary factor most likely associated with the maintenance of the differentiated phenotype of this tendon. Anatomy and Embryology 200: 55-64, 1999. IF= 1.554

P106-99 The epiphyseal cartilage and growth of long bones in Rana catesbeiana

Felisbino SL, Carvalho HF*

The structure of the epiphyseal cartilage of the bullfrog Rana catesbeiana and its role in the growth of long bones were examined. The epiphyseal cartilage was inserted into the end of a tubular bone shaft, defining three regions: articular cartilage, lateral articular cartilage and growth cartilage. Joining the lateral cartilage to the bone was a fibrous layer of periosteum, rich in blood vessels. Osteoblasts with alkaline phosphatase activity were found on the surface of the periosteal bone, which presented a fibrous non-mineralised tip. The growth cartilage was inside the bone. Chondrocyte hypertrophy was not associated with either calcification or endochondral ossification, in apparent contrast to the avian and mammalian models. Since bone extension depends on the intramembranous ossification of the periosteum, the growth cartilage is inside and not at the end of the bone and the cells in the growth cartilage show no columnar arrangement and separate in a direction perpendicular to the long bone axis, we conclude that the growth cartilage mainly contributes to the radial expansion of the bone.Tissue & Cell 31: 301-307, 1999. IF= 1.000

P107-99 Identification and distribution of type VI collagen in tendon fibrocartilages

Felisbino SL, Carvalho HF*

Structural and compositional variations are marked in tendon fibrocartilages.Type VI collagen was found in tensional and compressive areas of the plantaris longus tendon of the bullfrog, in the deep flexor tendon of dogs and rabbits, in the calcanear tendon and the suprapatela of rats and in the gastrocnemius tendon of chickens. The ATP treatment not only revealed the typical ladder-like aggregates of type VI collagen in the same areas identified by immunocytochemistry, but also demonstrated that type VI collagen forms a microfibrillar network around the fibrochondrocytes. Besides organizing groups of collagen fibrils, type VI collagen seems to assemble the pericellular matrix in tendon fibrocartilages, possibly through physical interactions with the large proteoglycans that are concentrated in the same area. Journal of Submicroscopic Cytology and Pathology 31: 187-195, 1999. IF= 0.690

P108-99 Larval aggregation and competition for food in experimental populations of Chrysomya putoria (Wied.) and Cochliomyia macellaria (F.) (Dipt., Calliphoridae)

Reis SF*, Von Zuben CJ, Godoy WAC

Aggregation has been suggested as a mechanism promoting coexistence in competing species, particularly those living in patchy resources, by changing the relative amounts of intra and interspecific competition. In this study we especifically test the relationship between aggregation and competition and its consequences for coexistence, using data from survival to adulthood in single- and double-species larval cultures of the introduced blowfly Chrysomya putoria and the native blowfly Cochliomyia macellaria. The reduction in C. macellaria survival rate in the presence of C. putoria suggests that the former species is the inferior competitor. The data on survival rates for both species in single- and double-species cultures are indicative of beneficial and deleterious levels of intra and interspecific aggregation on the efficiency of the larval feeding process. Journal of Applied Entomology 123: 485-489, 1999. IF= 0.366

P109-99 Virulence-associated factors of uropathogenic Escherichia coli strains isolated from pigs

Brito BG, Leite DS*, Linhares REC, Vidotto MC

Thirty one Escherichia coli strains isolated from pigs with urinary tract infections were investigated for presence of virulence factors and plasmid DNA profile. The most frequent virulence factors presented by these strains were mannose-resistant fimbriae, including P fimbriae and aerobactin production. The pap operon, detected by PCR, was found in 54.8% of strains, which is similar to its frequency in human strains. Other characteristics such as the presence of manosse-sensitive hemaglutinatinin, indicative od type 1 pili, and production of hemolysin, colicin and toxins were less frequent. No strains were positive for STa production. Plasmid profiles were variable among isolates from either the same or different farms. Revista de Microbiologia 65: 123-132, 1999. IF= 0.074


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