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Artigos
Publicados em Periódicos
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P020-00 Development of
cardiomyocite hypotrophy in rats under prolonged treatment
with a low dose of a nitric oxide synthesis inhibitor de Oliveira
CF, Cintra KA, Teixeira SA, De Luca IMS*, Antunes E, De Nucci
G Chronic administration of the nitric oxide synthesis inhibitor
N(omega)-nitro-L-arginine methyl ester (L-NAME) to rats causes
hypertension and morphological abnormalities in the heart,
consisting mainly of ventricular hypertrophy and foci of necrosis
and fibrosis. Since these phenomena have usually been described
with high or moderate doses of L-NAME, this study was undertaken
to evaluate the effects of a low dose of L-NAME on 2, 4 and
6 months of treatment. Our results show that chronic treatment
of rats with a low dose of L-NAME for prolonged periods (up
to 6 months) causes arterial hypertension accompanied by significant
reductions in heart weight, left ventricular weight indexes,
and cardiomyocyte size. European Journal of Pharmacology,
391(1-2):121-126, 2000 IF = 2,047 *e-mail: ideluca@obelix.unicamp.br
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| P021-00 Histologic
effect of mitomycin C on strabismus surgery in the rabbit Minguini
N, Monteiro de Carvalho KM, Akaishi PM, De Luca IMS* In order
to evaluate the efficiency of mitomycin C (MMC) in limiting
the postoperative inflammatory response and scarring after strabismus
surgery, a prospective, two-stage, masked, controlled trial
was conducted. In the first stage, the inflammatory response
at the extraocular muscle reattachment site was increased in
eight rabbits. In the second stage, MMC (0.4 mg/ml) was applied
during surgery to the eyes of 22 rabbits with inferior rectus
recession. Contralateral eyes (control) were treated with saline
solution. Seven weeks later, exenteration was performed, and
the sites of muscle reattachment were processed for histologic
examinations. There was no significant inhibitory effect of
MMC on the inflammatory response of treated eyes compared with
that of control eyes. The intraoperative use of MMC was not
effective in controlling the postoperative inflammatory response
in rabbit eyes after extraocular muscle surgery. Our data do
not support the hypothesis that MMC reduces posoperative adhesions
after strabismus surgery. Investigative Ophthalmology & Visual
Science 41(11): 3399-401, 2000 IF = 4,858 *e-mail: ideluca@obelix.unicamp.br |
| P022-00
GM-1 ganglioside treatment reduces motoneuron death after ventral
root avulsion in adult rats Oliveira ALR*, Langone F In the
present study we tested the neuroprotective and local anti-inflammatory
effects of monosialoganglioside (GM-1) after an initial 150mg/kg
body weight dose, followed by the daily administration of 100mg/kg
body weight for two weeks. The results showed a statistically
significant enhancement of surviving motoneurons with good morphological
preservation. Our results indicate that the administration of
GM-1 in high doses during the critical period of motoneuron
death after avulsion is neuroprotective, and diminishes local
inflammation. Neuroscience Letters, 293(2): 131-134, 2000. IF
= 2.085 *E-mail: alroliv@obelix.unicamp.br
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| P023-00 Differential
Schwann cell migration in adult and old mice: an in vitro study
Santos Jr A, Wada ML, Langone F, Oliveira ALR* It is well known
that SCs are essential for WD to occur, since they produce neurotrophic
factors and organize themselves within the so-called bands of
Büngner that precede axonal regeneration. Then, we have studied
in vitro, the influence of aging on the SC proliferation, migration
and viability. SCs were cultured from sciatic nerve explants
obtained from adult (six months old) and old (two years old)
C57BL/6J isogenic mouse. After 20 days of culture, the samples
were processed for immunohistochemistry using S100 antibody
for SC detection. The results showed that adult derived SCs
migration is stimulated with FCS and that this capacity is highly
diminished with aging. Brain Research 881(1): 73-76, 2000. IF
= 2.302 *E-mail: alroliv@obelix.unicamp.br |
| P024-00 Non-neuronal
cells are not the main limiting factor for the low axonal regeneration
in C57BL/6J mice Oliveira ALR*, Langone F The peripheral axonal
regeneration was investigated in adult male mice from C57BL/6J,
BALB/cJ and A/J strains and from their F1 descendants, using
a pre-degenerated nerve transplantation model. Donors had the
left sciatic nerve transected and two weeks later, a segment
of the distal stump was transplanted to the recipient. Four
weeks after transplantation the regenerated nerves were used
to record the total number of regenerated myelinated fibers
(TMF), diameter of myelinated fibers (FD) and myelin thickness
(MT). The highest TMF values were obtained in the groups where
C57BL/6J was the donor. When C57BL/6J was the graft donor, FD
was the highest and MT was not statistically different when
compared with the other groups. The morphometrical results reinforce
the idea that the Schwann cells and the nerve environment of
C57BL/6J provide enough support to the regenerative process.
Brazilian Journal of Medical and Biological Research 33(12):
1467-1475, 2000. IF = 0.517 *E-mail: alroliv@obelix.unicamp.br
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| P025-00 From
plaque to pretzel: fold formation and acetylcholine receptor
loss at the developing neuromuscular junction Marques MJ*, Conchello
J-A, Lichtman JW We have studied the maturation of the AChR
distribution and postsynaptic topography simultaneously by imaging
labeled AChRs at the mouse neuromuscular junction in a new way,
using reflected light confocal microscopy. We suggest that tension
between growing muscle fibers and the relatively inelastic synaptic
terminals that adhere to them causes the topographic features
of the postsynaptic membrane. This view provides a mechanical
explanation for gutters, folds, and the location of folds at
sites of neurotransmitter release. The Journal of Neuroscience
20(10): 3663-3675, 2000. IF = 8.955 *E-mail:marques@obelix.unicamp.br |
| P026-00 Inhibition
of acid phosphatase isoforms purified from mature soybean (Glycine
max) seeds Ferreira CV, Taga EM, Aoyama H* Several compounds
were studied as inhibitors of four soybean seeds acid phosphatase
isoforms (AP1, AP2, AP3A and AP3B), with p-nitrophenylphosphate
as substrate. Phosphate, o-vanadate, fluoride and molybdate
were competitive inhibitors. Under the same conditions, p-chloromercuribenzoate
and pyridoxal 5-phosphate had no significant effects, suggesting
that cysteine and lysine residues are not in the active site
of the four isoforms. Journal of Enzyme Inhibition 15: 403-410,
2000 IF = 1.295 *E-mail: aoyama@unicamp.br |
| P027-00
Cytotoxicity of okadaic acid and kinetic characterization of
protein tyrosine phosphatase activity in V79 fibroblasts Aoyama
H*, Melo PS, Granjeiro PA, Haun M, Ferreira CV Some kinetic
properties of a protein phosphatase obtained from V79 cells
were described. Apparent Km and specificity constant values,
for tyrosine phosphate (TyrP) as substrate, were determined.
Inhibitions were observed in the presence of 100 mM p-chloromercuribenzoate
(70%), 10 mM fluoride (30%), 10 mM phosphate (40%), 100 mM m-vanadate
(80%) and 100 mM o-vanadate (90%). Inhibition by vanadate and
low Km value for TyrP suggest that the V79 phosphatase is a
protein tyrosine phosphatase. The enzyme activity was stimulated
when the cells were treated with okadaic acid, a Serine/Threonine
protein phosphatase inhibitor. Pharmaceutical and Phrmacology
Communications 6: 331-334, 2000 *E-mail: aoyama@unicamp.br |
| P028-00 Variações
estruturais e florísticas da vegetação de carrasco do planalto
de Ibiapaba, estado do Ceará Araújo FS, Martins FR, Shepherd
GJ Revista Brasileira de Biologia 59(4): 663-678, 2000. *E-mail:
fmartins@unicamp.br;
george@unicamp.br |
| P029-00 Pleurothallis
fabiobarrosii, a new Brazilian species: morphological and genetic
data with notes on the taxonomy of Brazilian rupicolous Pleurothallis
Borba EL, Felix JM, Semir J, Solferini VN Lindleyana 15: 2-9,
2000. *E-mail: semir@unicamp.br;
solferin@unicamp.br
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