Administrativo, Biblioteca, Departamentos, IB, Informática,  Museu Graduação, Pós, Extensão Projetos, Laboratórios Serviços, Empresa Junior, Atletica, CAB, Links
Instituto de Biologia Unicamp







ABSTRACTA (3) 2000

Artigos Publicados em Periódicos

P060-00 Extended chromatin fibres: crystallinity, molecular order and reactivity to concanavalin-A Vidal BC* The reproducible formation of extended chromatin fibres, beads and superbeads was studied in chicken erythrocytes and mouse liver cells treated with NaCl and Triton X-100 solutions and positioned vertically, prior to staining with toluidine blue at pH 4.0, con-A binding and examination with polarization microscopy, or to observations at the electron microscopy level. DNA molecular order and crystallinity as assessed by optical ansiotropies as well as glycoprotein mannose/glucose residues were found to be well preserved in condensed and noncondensed chromatin after formation of the extended chromatin fibres. These results were associated with chromatin mobility data. Cell Biology International 24(10): 723-728, 2000. IF = 0.731 *E-mail:

P061-00 Image analysis of DNA fragmentation and loss in V79 cells under apoptosis Maria SS, Vidal BC*, Mello MLS* Image analysis of Feulgen-stained V79 cells after three days in culture was used to discriminate apoptotic cells and cells suspected to be undergoing apoptosis from control cells, based on parameters such as Feulgen-DNA content, degree of chromatin condensation and nuclear areas, in association with visual morphology. Three nuclear phenotypes were defined in terms of morphological characteristics and levels of chromatin condensation. Image analysis procedures proved to be a useful tool for discriminating cells in the early stages of apoptosis. Genetics and Molecular Biology 23: 109-112, 2000. IF = 0.250 *E-mail:,
P062-00 Chromatin organization in normal and cancer cells Mello MLS*, Vidal BC* A brief overview on basic concepts regarding chromatin organization and supraorganization in normal, transformed and tumorigenic cells was presented. Reference to some unsolved questions on morphological images and chromatin physiology relationships was emphasized. Journal of Women's Cancer 2(2): 73-78, 2000. *E-mail:,
P063-00 Nuclear phenotype changes after heat shock in Panstrongylus megistus (Burmeister) Garcia SL, Mello MLS*, Rodrigues VLCC, Garcia NL The nuclear phenotypes of Malpighian tubule epithelial cells of male nymphs of the blood-sucking insect, P. megistus, subjected to short- and long-duration heat shocks at 40oC were analyzed immediately after the shock and 10 and 30 days later. The frequency of the various nuclear phenotypes identified suggests that the forms of cell survival (unravelled heterochromatin, nuclear and cell fusion), present in this species, were not sufficient or efficient enough to protect all of the Malpighian tubule cells from the deleterious effects of the stress. Memórias do Instituto Oswaldo Cruz 95(2): 271-277, 2000. IF = 0.636 *E-mail:
P064-00 Changes in nuclear phenotypes following cold shocks in Panstrongylus megistus (Burmeister) Garcia SL, Mello MLS*, Garcia NL, Rodrigues VLCC The nuclear phenotypes of Malpighian tubule epithelial cells of P. megistus were studied immediately after a short (1 h) cold shock at 0oC, and 10 and 30 days later. The objective was to compare the response to a cold shock with that reported to occur after hyperthermia, in order to provide insight into the cellular effect of cold in this blood-sucking insect species. Most of the changes detected have also been found in heat-shocked specimens, except for nuclear fusion which was found to be less effective or necessary in cold-shocked specimens than that induced by heat shock. Heat shock proteins were assumed as the best candidates for effective protection of the cells and the insects from the drastic damage caused by low temperature shocks. Memórias do Instituto Oswaldo Cruz 95(6): 893-898, 2000. IF = 0.636 *E-mail:
P065-00 DNA fragmentation in programmed cell death in nucleate erythrocytes: a cytochemical analysis Mello MLS*, Maria SS, Schildknecht PHPA, Grazziotin NA The frequency and responsiveness to the TUNEL assay were used to compare the DNA fragmentation pattern associated with programmed cell death in nucleate erythrocytes of the circulating blood in different species in which the life span and/or metabolic activities of the red blood cells vary considerably. The results reported here, especially in Rana catesbeiana indicate that further studies are required for a better understanding of cell death in vertebrate nucleate erythrocytes. Acta Histochemica et Cytochemica 33(5): 15-19, 2000. IF = 0.517 *E-mail:
P066-00 Antiulcerogenic effect of a hydroalcoholic extract and its organic fractions of Neurolaena lobata (L.) Gracioso JS, Hiruma-Lima CA, Brito ARMS* Neurolaena lobata is a species used widely in Caribbean folk medicine to treat gastric pain and ulcers. Fractions of a hydroalcoholic extract of N. lobata aerial parts were studied for their ability to prevent ulceration of the gastric mucosa. In the stress-induced gastric model these fractions produced a significant reduction of gastric lesion formation. HE, HxF and CIF fractions also reduced significantly the gastric lesions induced by the combination of indomethacin and bethanechol, and the ulcers induced by HCl/ethanol solution (P < 0.05). The pylorus-ligature experiment demonstrated that the studied fractions changed significantly the gastric juice parameters. In the animals pre-treated orally with the HxF fraction, prostaglandin synthesis was increased significantly and free mucus production was increased by 54% in the gastric mucosa (P < 0.001). The data suggest that the HE and mainly the HxF fractions from N. lobata present a significant anti-ulcer effect when assessed in these ulcer-induced models. It is assumed that the mechanism underlying this antiulcerogenic effect is possibly related to an increased activity of the defensive mechanisms of the stomach, such as prostaglandin synthesis and mucus production. Phytomedicine 7(4): 283-289, 2000. IF = 0.185 *E-mail:
P067-00 The juice of fresh leaves of Boerhavia diffusa L. (Nyctaginaceae) markedly reduces pain in mice Hiruma-Lima CA, Gracioso JS, Bighetti EJB, Robineou LG, Brito ARMS* The decoction or juice of leaves of Boerhavia diffusa L. (Nyctaginaceae) is used in Martinican folk medicine for its analgesic and anti-inflammatory properties. Here we studied the acute oral toxicity of a crude extract obtained from a lyophilized decoction (DE) and from the juice (JE) of fresh leaves. No signs of toxicity were found up to the dose of 5000 mg/kg (p.o.) in mice. The extracts of B. diffusa were also investigated for their anti-edematogenic effect on carrageenan-induced edema in mice. However, neither extract inhibited the paw edema induced in mice (P > 0.05). The results show that the active antinociceptive principle of B. diffusa is present mainly in the juice of fresh leaves and has a significant antinociceptive effect when assessed in the pain models used. The mechanism underlying the analgesic effect of fresh leaves of B. diffusa is possibly related to interaction with the opioid system. Journal of Ethnopharmacology 71(1-2): 267-274, 2000. IF = 0.687 *E-mail:
P068-00 Gastroprotective effect of essential oil from Croton cajucara Benth. (Euphorbiaceae) Hiruma-Lima CA, Gracioso JS, Rodriguez JA, Haun M, Nunes DS, Brito ARMS* The gastroprotective activity of the essential oil from the bark of Croton cajucara Benth was assessed in three different models of experimentally induced gastric ulcer in mice. At oral dose of 100 mg/kg the essential oil reduced gastric lesions induced by hypothermic restraint stress and HCl/ethanol significantly. In the HCl/ethanol model a dose-dependent gastroprotective effect was found. Significant changes in gastric parameters were found after intraduodenal administration of essential oil under ligated pylorus conditions. Acute toxicity of essential oil was assessed in mice. The LD50 values were 9.3 and 680 mg/kg for oral and intraperitoneal administrations, respectively. A dose-dependent cell viability inhibition was found in V79 fibroblast cell cultures with an IC50 of 22.9 mg/ml. These results support the pharmacological use of the essential oil from Croton cajucara. Journal of Ethnopharmacology 69(3): 229-234, 2000. IF = 0.687 *E-mail:
P069-00 Locomotor response to an openfield during C57BL/6J active and inactive phases: differences dependent on conditions of illumination Valentinuzzi VS, Buxton OM, Chang AM, Scarbrough K, Ferrari EAM*, Takahashi JS, Turek F Time of day has proven to be a source of variability in diverse behavioral measures. Knowledge of the pattern of this temporal effect and its exogenous or endogenous origin is essential for a precise description of any behavior. Here we analyzed the effect of the external light-dark cycle and the internal rest-activity rhythm on the response of C57BL/6J mice to a novel environment: 1. animals maintained in a 12:12 light:dark cycle were tested in an openfield at six different times of day; 2. the animals were kept in dim green light where entrainment was maintained by a skeleton photoperiod (two15-minute bright light pulses separated by 12 hours of green dim light). No effect of circadian phase on ambulation or habituation of the response to the openfield was observed. It is suggested that spatial novelty is equally arousing regardless of circadian phase and that the conditions of illumination can dramatically alter the response to a novel environment. Physiology & Behavior 69: 269-275, 2000. IF = 1.285 *E-mail:

Diretoria do Instituto de Biologia

Fone: (19) 37886358 / 37886361
Caixa Postal 6109 - CEP 13.084-971 - Campinas - SP