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Instituto de Biologia Unicamp







ABSTRACTA (3) 2000

Artigos Publicados em Periódicos

P070-00 Caffeine content on Ethiopian Coffea arabica beans Silvarolla MB, Mazzafera P* The coffee germplasm bank of the Instituto Agronômico de Campinas has many Coffea arabica accessions from Ethiopia, which is considered the primary center of genetic diversity in coffee plants. An evaluation of the caffeine content of beans from 99 progenies revealed intra- and inter-progeny variability. In 68 progenies from Kaffa region it was found caffeine values in the range 0.46-2.82% (mean value 1.18%), and in 22 progenies from Illubabor region these values ranged from 0.42 to 2.90% (mean value 1.10%). This variability could be exploited in a breeding program aimed at producing beans with a low caffeine content. Genetics and Molecular Biology 23(1): 213-215, 2000. IF = 0.250 *E-mail:

P071-00 Caffeine formation by suspension cultures of C. dewevrei Sartor RM, Mazzafera P* The low caffeine content in leaves of C. dewevrei is due to a low biosynthesis associated with a fast degradation, and high biosynthesis and low degradation confer a higher content in C. arabica. Here we observed that cell cultures of C. dewevrei recovered the ability to synthesize caffeine almost in similar levels of C. arabica cultures. Tracer experiments with 14CO2 showed an accumulation of radioactivity in caffeine and metabolites, indicating an active biosynthesis. When the cultures were fed with labelled caffeine most of the radioactivity was recovered in caffeine, indicating that although active, degradation was not so efficient as in leaves. Brazilian Archives of Biology and Technology 43(1): 61-69, 2000. IF = 0.065 *E-mail:
P072-00 Compositional changes of proteins and amino acids in germinating coffee seeds Shimizu MM, Mazzafera P* Coffea arabica seeds were germinated for six weeks and changes in amino acids and proteins were investigated. Free amino acids were reduced during germination and protein remained constant. SDS?PAGE showed that legumin-like proteins became less stained in the last weeks. ASP, GLU, ASP, ALA and LYS were the major amino acids, although SER and GLN were also significant. Except for TYR, which increased with germination, all other amino acids were reduced. Analysis of the amino acid composition of the total soluble protein showed GLU/GLN and GLY as the main amino acids. However, other amino acids such as LEU, ASP/ASN, ALA, LYS, SER were also found in reasonable amounts. Brazilian Archives of Biology and Technology 43(3), 2000. IF = 0.065 *E-mail:
P073-00 Caffeine does not protect coffee against the leaf miner Perileucoptera coffeella Guerreiro Filho O, Mazzafera P* Coffee plants containing different levels of caffeine were exposed to oviposition of the insect and the caffeine content and damaged leaf area were evaluated. In addition, plants were exposed to the insect but one leaf of each pair was protected from oviposition with paper bags. In another experiment, leaf disks from plants with known susceptibility to the leaf miner were infiltrated with caffeine solutions and exposed to oviposition. None of the experiments established a correlation between reduction of leaf damage and caffeine content of the tissue. Journal of Chemical Ecology 26(6): 1447-1464, 2000. IF = 1.653 *E-mail:
P074-00 Atividade da enzima redutase do nitrato em algodoeiro submetido ao déficit hídrico e posterior recuperação da turgescência. Marur CJ, Mazzafera P*, Magalhães AC A atividade da redutase do nitrato foi estudada em plantas de algodão (Gossypium hirsutum, L.), cvs IAC 20 e IAC 13-1, submetidas a déficits crescentes de água, e posterior recuperação da turgescência. A atividade sofreu redução quando o Ya foi menor que -1,5 MPa. Não ocorreram diferenças entre os dois cultivares em relação ao estabelecimento do estresse e padrão de recuperação da turgescência. O ensaio in vivo da atividade mostrou ser um método sensível para a avaliação do efeito do estresse hídrico sobre o crescimento das plantas, constituindo-se em um parâmetro indicativo do nível geral do metabolismo na planta. Scientia Agricola 57(2): 277-281, 2000. *E-mail:
P075-00 Characterization of polyphenol oxidase in coffee Mazzafera P*, Robinson SP Polyphenol oxidase (PPO) was characterized in partially purified extracts of leaves (PPO-L) and fruit endosperm (PPO-E) of coffee (Coffea arabica L.) regarding induction by wounding and methyl jasmonate, activation by protease treatment, stimulation by SDS, pH, assay temperature, substrate, heat stability, inhibitors, Km and molecular weight. Activity-stained SDS-PAGE gels and western blots probed with PPO antibodies suggested the existence of a 67 kDa PPO which is susceptible to proteolytic cleavage that generates a 45 kDa active form. Phytochemistry 55(4): 285-296, 2000. IF = 1.106 *E-mail:
P076-00 Supercritical carbon dioxide extraction of methylxanthines from maté tea leaves Saldaña MDA, Mohamed RS, Mazzafera P* Experimental data on the supercritical CO2 extraction of caffeine, theophylline and theobromine from herbal maté tea were obtained using high pressure extraction equipment that allows adequate control of temperature and pressure. The continuous extraction/fractionation of maté tea leaves, Ilex paraguariensis in natura using carbon dioxide was carried out at 313.2 and 343.2 K and pressures of 13.8 and 25.5 MPa. Extraction/fractionation curves revealed the large influence of temperature and pressure on extraction yield. CO2 was also found to show a higher selectivity for caffeine than for theophylline and theobromine. Brazilian Journal of Chemical Engineering 17(3): 251-259, 2000. *E-mail:
P077-00 A role for trigonelline during imbibition and germination of coffee seeds Shimizu MM, Mazzafera P* Trigonelline was investigated as a NAD reserve during germination of Coffea arabica seeds. The data show that in seeds there is a significant conversion of trigonelline to NAD, although a concomitant biosynthesis of the alkaloid is also observed. It is concluded that trigonelline is a reserve molecule for NAD biosynthesis but this function may be limited to very early stages of germination or restricted to specific tissues such as the embryo. Plant Biology 2: 1-7, 2000. *E-mail:
P078-00 Labidura riparia (Dermaptera: Labiduridae) from Brazil: karyotype and C-banding Avancini RMP, Recco-Pimentel SM, Prado AP* The karyotype of Labidura riparia from Brazil is 2n=5A+XY for males and 2n=5A+XX for females. The chromosomes can be grouped into 3 large and 3 small pairs, the latter including the sex chromosomes. The Y chromosome is entirely C-band positive and remains heteropycnotic during most of the division cycle. The X chromosome, which is longer than the Y chromosome, presents no C-banding. The longest chromosome of the complement has very noticeable C-bands at one of its terminal. These results are very similar to the karyotype described for L. riparia in the USA. The most significant dissimilarity is that chromosome number 1 is about 20% longer than chromosome 2, a feature not observed in the karyotypes of L. riparia from other regions of the world. In addition, the difference in size between the chromosomes X and Y of Brazilian and US L. riparia is not as large as that for other populations. Cytologia 65: 93-96, 2000. *E-mail:
P079-00 Ocorrência de Trichopria sp. (Hymenoptera: Diapriidae) atacando pupas de Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae) na granja Monteiro MR, Prado AP* This work was carried out in a poultry house (Granja Capuavinha), Monte Mor, State of São Paulo, from February 1991 to April 1992 with the purpose of studying the synanthropic flies their parasitoids. The pupae were extrated from manure using a water-flotation method. Berlese-Tullgren's funel was used to extract parasitoids and fly larvae. Each pupa was kep in a small transparent glass vial for 35 days until the emergence of the fly or parasitoid. After this period, the pupae that did not yield adult flies or parasitoids were dissected. The following species of fly were collected: Chrysomya putoria (Wiedman), Muscina stabulans (Fallén), Musca domestica L., Fannia pusio (Stein). In addition several species of Sepsidae were also collected. The following species of parasitoids emerged from fly pupae collected: Spalangia gemina (Boucek), S. cameroni (Perkins), Muscidifurax raptoroides Kogan & Legner, Pachycrepoideus vindemiae (Rondani), Nasonia vitripennis (Walker) and Trichopria sp. (Diapriidae). Tachinaephagus zealandicus (Ashmead) (Encyrtidae) a larval pupal parasitoid also ocurred. This is the first report of Trichopria sp., a gregarious parasitoid, attacking pupae of C. putoria. About 13% of parasitoids did not eclode successfuly from the parasitoid pupae which may indicate a recent association between Trichopria sp. and C. putoria in Brazil. Anais da Sociedade Entomologica do Brasil 29(1): 159-167, 2000. *E-mail:

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