Artigos Publicados em Periódicos

P090-00 Protective immunity induced by a Trypanosoma cruzi soluble extract antigen in experimental Chaga's Disease. Role of Interferon ? Garcia CAAC, Oliveira EC, Sakurada JK, Santos LMB* CBA/J mice can be protected lethal infection with Trypanosoma cruzi by treatment using T.cruzi soluble extract antigen (TCSE). In vivoatration of TCSE (400 µg/mouse) into naive mice increased the cellular proliferative response to Con A and elevated the levels of IFN-?. These results suggest that depending on the level, cytokine production results in the control of replication of the parasite in experimental Chaga's disease. Immunological Investigations 29(1): 1-12, 2000. IF = 1.084 *E-mail: leonilda@obelix.unicamp.br

P091-00 Clinical relevance and virulence factors of pigmented Serratia marcescens Carbonell GV, Della Colleta HHM, Yano T*, Darini ALC, Levy CE, Fonseca BAlL Pigmented Serratia marcescens isolated in a Brazilian hospital were respsect to frequency of isolation, serotyping, antibiotic resistance and virulence factors. The serotype most frequent was 06K14 (53%) and all isolates were resistant to ampicilin, cephalothin and tetracycline. The majority of the isolates (92%) were resistant to the action of human serum and all produced cytotoxins on Vero, CHO, Hep-2 and HeLa cells. These isolates were virulent for mice (LD50 = 107 bacteria/ml-1) and showed virulence factors, but were isolated with low frequency (3.4%) and caused infection in only 31% of cases. Analysis of serotypes phage tuping and chromosomal DNA revealed at least 13 unrelated strains among pigmented S. Marcescens. In conclusion, this work describes a low frequency os isolation of pigmented S. Marcecens from clinical specimens, indicating that non-pigmented strains are clinically more significant. FEMS Immunology and Medical Microbiology. 28:143-149, 2000. IF = 1.329 *E-mail: tyano@obelix.unicamp.br

P092-00 Purification and characterization of the fimbria F18ac (213P) isolated from enterotoxigenic Escherichia coli (ETEC) Amorin CRN, Matsuura MSA, Rosa JC, Greener LJ, Leite DS, Yano T* The adhesin F18ac purified on Sepharose CL 4B column chromatography and SDS-PAGE stained with Coomasie Blue and Western blotin using specific anti-F18ac serum presented one band of 17Kda. Gold immunolabeling revealed that this adhesin has a fimbrial structure on the bacterial surface. The first 27 amino acid residues of the N-terminal portion of the adhesin F18ac, showed 92.5% homology (25 amino acids) with the F107 (F18ac) fimbriae. Veterinary Microbiology. 76:41-49, 2000. IF = 1.377 *E-mail: tyano@obelix.unicamp.br

P093-00 Pathogenic characteristics of Escherichia coli strains isolated from newborn piglets with diarrthea in Brazil Martins MF, Matinez-Rossi NM, Ferreira A, Brocchi M, Yano T*, Pestana de Castro AF, Silveira WD Ninety-one Escherichia coli isolated from diarrheic and normal faeces of newborn piglets from three states of Brazil were assessed for phenotypic and genotypic characteristics associated with patogenic processes. These isolates expressed fimbriae F18ac and type 1, but nor fimbriae K88, K99, 987P or F41. Gene for toxins either individually or combined were found to be present in most of the diarrheic strains (65,7%) and in 42.8% of the non-diarrheic ones. Colicin, hemolysin and aerobactin were also found to be prodiced by some strains from both sources. Because of the great variety of biological characteristics associated with different illness process, we suggest that, in Brazil, pigs may act as a reservoir for transmission of Escherichia coli strains to other animals. Veterinary Microbiology 76:51-59, 2000. IF = 1.377 *E-mail: tyano@obelix.unicamp.br

P094-00 Replication of classical infectious bursal disease virus in the chicken embryo related (CER) cell line Cardoso TC, Rahal P, Pilz D, Teixeira MCB, Arns CW* Infectious bursal disease (IBD) is an acute, highly contagious viral disease. The diagnosis of IBD depends on time consuming and costly procedures, like virus isolation on chick embryos and histopathological examination. A double antibodies sandwich ELISA (DAS-ELISA), immunoperoxidase and reverse transcription polimerase chain reaction (RT/PCR) were applied to detect classical IBDV after three blind passages of the Lukert strain on chicken embryo related (CER) cell monolayer after different periods of infection. Cytophatic effects were most evident after 12 h post-infection (p.i) but were observed at 6 h p.i. The maximum discrimination between IBDV-infected and uninfected cells suspension obtained by the use of DAS-ELISA for virus detection was determined. Since 6 h p.i it was possible to detect the viral RNA. The results show that CER cell can be used for classical IBDV propagation confirmed by the DAS-ELISA, immunoperoxidase and RT/PCR assay. Avian Pathology 29: 213-219, 2000. IF = 0.845 *E-mail : verinaud@obelix.unicamp.br

P095-00 Subtypes of intimin among Attaching and Effacing Escherichia coli from calves with diarrhea in Brazil Aidar L, Penteado AS, Trabulsi LR, Blanco M, Blanco J, Pestana de Castro AF* Canadian Journal of Veterinary Research 64: 15-20, 2000. IF = 0.811 *E-mail: apestana@obelix.unicamp.br

P096-00 Morphological aspects of cultured rat seminal tubules in the presence of calf fetal serum and folicle-stimulating hormone Lombello CB, Dolder H* Journal of Submicroscopic Cytology and Pathology 32 : 11-18, 2000. IF = 0.701 *E-mail: heidi@unicamp.br

P097-00 Sperm ultrastructure of the honey bee (Apis mellifera) (L) (Hymenoptera, Apidae) with emphasis on the nuclear flagellum region Lino Neto J, Dolder H* Tissue and Cell 32 : 322-327, 2000. IF = 1.028 *E-mail: heidi@unicamp.br

P098-00 Structure and ultrastructure of the spermatozoa of Trichogramma pretiosum Riley and T. atopovirilia Oatman and Platner (Hymenoptera: Tricogrammatidae) Lino Neto J, Dolder H* Acta Zoologica 81: 205-211, 2000. IF = 0.897 *E-mail: heidi@unicamp.br

P099-00 Deletion mapping of the potyviral helper component-proteinase reveals two regions involved in RNA binding Urcuqui-Inchima S, Maia IG, Arruda P*, Haenni AL, Bernardi F The Potyvirus helper component-proteinase (HC-Pro) binds nonspecifically to single-stranded nucleic acids with a preference for RNA To delineate the regions of the protein responsible for RNA binding, deletions were introduced into the full-length Potato potyvirus Y HC-Pro gene carried by an Escherichia coil expression vector. The corresponding proteins were expressed as fusions with the maltose-binding protein, purified, and assayed for their RNA-binding capacity. The results obtained by UV cross-linking and Northwestern blot assays demonstrated that the N- and C-terminal regions of HC-Pro are dispensable for RNA binding. They also revealed the presence of two independent RNA-binding domains (designated A and B) located in the central part of HC-Pro. Domain B appears to contain a RNP motif typical of a large family of RNA-binding proteins involved in several cellular processes. The possibility that domain a consists of an RNP domain is discussed and suggests that HC-Pro could constitute the first example of a plant viral protein belonging to the RNP-containing family of proteins. Virology 268: 104-111, 2000. IF = 3.548 *E-mail: parruda@unicamp.br

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