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Instituto de Biologia Unicamp







ABSTRACTA (3) 2000

Artigos Publicados em Periódicos

P120-00 Aggrecan structure in amphibian cartilage Covizi DZ, Carvalho HF* The structure of the large proteoglycan present in the bullfrog epiphyseal cartilage was studied by immunochemical and biochemical methods. The isolated monomer showed a polydisperse behavior on Sepharose CL2B. Chondroitin sulfate chains were identified by HPLC analysis of the products formed by chondroitinase digestion and mercuric acetate treatment and keratan sulfate was identified by the use of two monoclonal antibodies in Western blots after chondroitinase ABC treatment. A keratan sulfate-rich region (~110 kDa) was isolated by sequential treatment with chondroitinase ABC and proteases. Antibodies were employed in Western blotting experiments. Domain-specific antibodies revealed the presence of immunoreactive sites corresponding to G1/G2 and G3 globular domains and the characterization of this large proteoglycan as aggrecan. The results indicate the high conservation of the aggrecan domain structure in this lower vertebrate. Brazilian Journal of Medical and Biological Research 33: 1403-1412, 2000. IF = 0.517 *E-mail:

P121-00 Heat shock-induced apoptosis in germ line cells of Triatoma infestans Klug Mello MLS*, Maria SS, Tavares MCH Apoptosis was investigated in male germ line cells from 5th instar nymphs of T. infestans 7 and 30 days after heat shock exposure at 40oC for 1 hour. The preparations were subjected to the Feulgen reaction and the TUNEL assay. DNA fragmentation elicited by heat shock was found in spermatogonial chromatin and chromosomes, spermatids and sperm cells. Spermatogonia was most affected 7 days after the shock, whereas some spermatids and sperm cells were affected only 30 days after heat shock. In some cases where DNA fragmentation occurred towards the end of spermatogenesis, cellular differentiation was unaffacted by heat shock. Genetics and Molecular Biology 23(2): , 2000. IF = 0.250 *E-mail:
P122-00 Behaviourally driven gene expression reveal song nuclei in hummingbird brain Jarvis ED, Ribeiro S, Silva ML, Ventura D, Vielliard J*, Mello CV Nature 406: 628-632, 2000. IF = 29.491 *E-mail:
P123-00 Evaluation of the gastroprotective activity of cordatin, a diterpene isolated from Aparisthmium cordatum (Euphorbiaceae) Hiruma-Lima CA, Gracioso JS, Toma W, De Paula ACB, Almeida AB, Brasil DSB, Muller AH, Brito ARMS* Biol. Pharm. Bulletin 23(12), 2000. *E-mail:
P124-00 Eimeria curvata n.sp. (Apicomplexa: Columbidae) in Columbina talpacoti and Scardafella squammata (Aves: Columbidae) from Brazil Adriano EA, Thyssen PJ, Cordeiro NS* Eimeria curvata is a new coccidian described in the doves Columbina talpacoti and Scardafella squammata from western of State of São Paulo, Brazil. Oocists, sporocysts and sporozoites are described here. The prevalence of E. curvata n.sp. was 17.4% and 12.8% in C. talpacoti and S. squammata respectively. Memórias do Instituto Oswaldo Cruz 95(1): 53-55, 2000. IF = 0.636 *E-mail:
P125-00 The occurrence of aristolochic acids in Neotropical Troidine swallowtails (Lepidoptera: Papilionidae) Klitzke CF, Brown Jr KS* Analysis of individuals of 17 troidine species demonstrated the presence of aristolochic acids in these butterflies, in varying concentrations. Although aristolochic acids do not occur in Aristolochia galeata leaves, they were present in Battus polydamas larvae reared on this plant species, and therefore may be synthesized by the larvae from chemical precursors in the leaves. The concentrations found in each species are compared among each other and with other results in previously published papers. Chemoecology 10 (2): 99-102, 2000. *e-mail:
P126-00 Isolamento e identificação de amostras do vírus da doença infecciosa da Bursa Simoni CI, Fernandes CCC, Silva CE, Marinho NMSQ, Gama ENC, Tessari AGM, Castro AMBN, Arns CW* Revista Brasileira de Ciência Avícola, Suplemento 2, pg. 89, 2000 *E-mail:
P127-00 Purificação e detecção do Pneumovírus Aviário isolado no Brasil (SHS-BR-121) através da técnica de Microscopia eletrônica Coswig, LT, Monteiro MCGB, Arns CW* Revista Brasileira de Ciência Avícola, Suplemento 2, pg. 91, 2000 *E.mail:
P128-00 Lipid chain dynamics in stratum corneum studied by spin label electron paramagnetic resonance Alonso A*, Meirelles NC, Tabak M The lipid chain motions in stratum corneum (SC) membranes have been studied through electron paramagnetic resonance (EPR) spectroscopy of stearic acid spin-labeled at the 5th, 12th and 16th carbon atom positions of the acyl chain. The lower fluidity observed for the corneocyte envelope suggests a major contribution of this lipid domain to the barrier function of SC. Changes in the activation energy for reorientational diffusion of the 16-DSA spin label showed apparent phase transitions around 54°C, for the three SC samples. Some lipid reorganization occurring in SC above 54°C is sensitive to the presence of the extractable intercellular lipids, being different in the lipid-depleted sample as compared to native SC and lipid dispersion. The results contribute to the understanding of alkyl chain packing and mobility in the SC membranes involved in the mechanisms that control the permeability of different compounds through skin, suggesting an important involvement of the envelope in the skin barrier. Chemistry and Physics of Lipids 104 (2): 101-111, 2000. IF = 1.266 *E-mail:
P129-00 Membrane effects of trifluoperazine, dibucaine and praziquantel on human erythrocytes Malheiros SVP, Brito MA, Brites D*, Meirelles NC Trifluoperazine (TFP) is a potent antipsychotic agent, dibucaine (DBC) is a local anaesthetic and praziquantel (PZQ) is a highly effective agent against schistosomiasis. The present work was conducted to (i) investigate the cytotoxic effects of TFP, DBC and PZQ on human erythrocyte membranes; and (ii) compare the alterations induced by the cationic drugs (TFP and DBC) with those induced by the uncharged compound (PZQ), in an attempt to have a better insight on the pathways of each drug-membrane interaction. Our results showed that PZQ induces the same morphological alterations (stomatocytosis) as TFP and DBC. Haemolysis was shown to vary with the drug used and to be concentration-dependent. At a fixed rate of 50% haemolysis, TFP induced a ~ 2-fold increment in the elution of cholesterol when compared with that produced by DBC (P < 0.05). The different effects induced by TFP, DBC and PZQ on erythrocyte morphology, haemolysis and lipid exfoliation are related to the physical and chemical characteristics of each compound, suggesting that distinct cell membrane interaction pathways lead to drug-specific mechanisms of cytotoxicity. Chemico-Biological Interations 126:(2) 79-95, 2000. IF = 1.887 *E-mail:

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