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Artigos
Publicados em Periódicos
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P120-00 Aggrecan structure
in amphibian cartilage Covizi DZ, Carvalho HF* The structure
of the large proteoglycan present in the bullfrog epiphyseal
cartilage was studied by immunochemical and biochemical methods.
The isolated monomer showed a polydisperse behavior on Sepharose
CL2B. Chondroitin sulfate chains were identified by HPLC analysis
of the products formed by chondroitinase digestion and mercuric
acetate treatment and keratan sulfate was identified by the
use of two monoclonal antibodies in Western blots after chondroitinase
ABC treatment. A keratan sulfate-rich region (~110 kDa) was
isolated by sequential treatment with chondroitinase ABC and
proteases. Antibodies were employed in Western blotting experiments.
Domain-specific antibodies revealed the presence of immunoreactive
sites corresponding to G1/G2 and G3 globular domains and the
characterization of this large proteoglycan as aggrecan. The
results indicate the high conservation of the aggrecan domain
structure in this lower vertebrate. Brazilian Journal of Medical
and Biological Research 33: 1403-1412, 2000. IF = 0.517 *E-mail:
hern@unicamp.br
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| P121-00
Heat shock-induced apoptosis in germ line cells of Triatoma
infestans Klug Mello MLS*, Maria SS, Tavares MCH Apoptosis was
investigated in male germ line cells from 5th instar nymphs
of T. infestans 7 and 30 days after heat shock exposure at 40oC
for 1 hour. The preparations were subjected to the Feulgen reaction
and the TUNEL assay. DNA fragmentation elicited by heat shock
was found in spermatogonial chromatin and chromosomes, spermatids
and sperm cells. Spermatogonia was most affected 7 days after
the shock, whereas some spermatids and sperm cells were affected
only 30 days after heat shock. In some cases where DNA fragmentation
occurred towards the end of spermatogenesis, cellular differentiation
was unaffacted by heat shock. Genetics and Molecular Biology
23(2): , 2000. IF = 0.250 *E-mail: mlsmello@unicamp.br |
| P122-00
Behaviourally driven gene expression reveal song nuclei in hummingbird
brain Jarvis ED, Ribeiro S, Silva ML, Ventura D, Vielliard J*,
Mello CV Nature 406: 628-632, 2000. IF = 29.491 *E-mail: jacques@unicamp.br |
| P123-00
Evaluation of the gastroprotective activity of cordatin, a diterpene
isolated from Aparisthmium cordatum (Euphorbiaceae) Hiruma-Lima
CA, Gracioso JS, Toma W, De Paula ACB, Almeida AB, Brasil DSB,
Muller AH, Brito ARMS* Biol. Pharm. Bulletin 23(12), 2000. *E-mail:
abrito@unicamp.br |
| P124-00
Eimeria curvata n.sp. (Apicomplexa: Columbidae) in Columbina
talpacoti and Scardafella squammata (Aves: Columbidae) from
Brazil Adriano EA, Thyssen PJ, Cordeiro NS* Eimeria curvata
is a new coccidian described in the doves Columbina talpacoti
and Scardafella squammata from western of State of São Paulo,
Brazil. Oocists, sporocysts and sporozoites are described here.
The prevalence of E. curvata n.sp. was 17.4% and 12.8% in C.
talpacoti and S. squammata respectively. Memórias do Instituto
Oswaldo Cruz 95(1): 53-55, 2000. IF = 0.636 *E-mail: nsilvac@unicamp.br |
| P125-00
The occurrence of aristolochic acids in Neotropical Troidine
swallowtails (Lepidoptera: Papilionidae) Klitzke CF, Brown Jr
KS* Analysis of individuals of 17 troidine species demonstrated
the presence of aristolochic acids in these butterflies, in
varying concentrations. Although aristolochic acids do not occur
in Aristolochia galeata leaves, they were present in Battus
polydamas larvae reared on this plant species, and therefore
may be synthesized by the larvae from chemical precursors in
the leaves. The concentrations found in each species are compared
among each other and with other results in previously published
papers. Chemoecology 10 (2): 99-102, 2000. *e-mail: ksbrown@unicamp.br |
| P126-00
Isolamento e identificação de amostras do vírus da doença infecciosa
da Bursa Simoni CI, Fernandes CCC, Silva CE, Marinho NMSQ, Gama
ENC, Tessari AGM, Castro AMBN, Arns CW* Revista Brasileira de
Ciência Avícola, Suplemento 2, pg. 89, 2000 *E-mail: arns@unicamp.br |
| P127-00
Purificação e detecção do Pneumovírus Aviário isolado no Brasil
(SHS-BR-121) através da técnica de Microscopia eletrônica Coswig,
LT, Monteiro MCGB, Arns CW* Revista Brasileira de Ciência Avícola,
Suplemento 2, pg. 91, 2000 *E.mail: arns@unicamp.br |
| P128-00
Lipid chain dynamics in stratum corneum studied by spin label
electron paramagnetic resonance Alonso A*, Meirelles NC, Tabak
M The lipid chain motions in stratum corneum (SC) membranes
have been studied through electron paramagnetic resonance (EPR)
spectroscopy of stearic acid spin-labeled at the 5th, 12th and
16th carbon atom positions of the acyl chain. The lower fluidity
observed for the corneocyte envelope suggests a major contribution
of this lipid domain to the barrier function of SC. Changes
in the activation energy for reorientational diffusion of the
16-DSA spin label showed apparent phase transitions around 54°C,
for the three SC samples. Some lipid reorganization occurring
in SC above 54°C is sensitive to the presence of the extractable
intercellular lipids, being different in the lipid-depleted
sample as compared to native SC and lipid dispersion. The results
contribute to the understanding of alkyl chain packing and mobility
in the SC membranes involved in the mechanisms that control
the permeability of different compounds through skin, suggesting
an important involvement of the envelope in the skin barrier.
Chemistry and Physics of Lipids 104 (2): 101-111, 2000. IF =
1.266 *E-mail: secrbioq@unicamp.br |
| P129-00
Membrane effects of trifluoperazine, dibucaine and praziquantel
on human erythrocytes Malheiros SVP, Brito MA, Brites D*, Meirelles
NC Trifluoperazine (TFP) is a potent antipsychotic agent, dibucaine
(DBC) is a local anaesthetic and praziquantel (PZQ) is a highly
effective agent against schistosomiasis. The present work was
conducted to (i) investigate the cytotoxic effects of TFP, DBC
and PZQ on human erythrocyte membranes; and (ii) compare the
alterations induced by the cationic drugs (TFP and DBC) with
those induced by the uncharged compound (PZQ), in an attempt
to have a better insight on the pathways of each drug-membrane
interaction. Our results showed that PZQ induces the same morphological
alterations (stomatocytosis) as TFP and DBC. Haemolysis was
shown to vary with the drug used and to be concentration-dependent.
At a fixed rate of 50% haemolysis, TFP induced a ~ 2-fold increment
in the elution of cholesterol when compared with that produced
by DBC (P < 0.05). The different effects induced by TFP, DBC
and PZQ on erythrocyte morphology, haemolysis and lipid exfoliation
are related to the physical and chemical characteristics of
each compound, suggesting that distinct cell membrane interaction
pathways lead to drug-specific mechanisms of cytotoxicity. Chemico-Biological
Interations 126:(2) 79-95, 2000. IF = 1.887 *E-mail: secrbioq@unicamp.br
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