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ARTIGOS ACEITOS PARA PUBLICAÇÃO
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A001-01 The effect of
therapeutic ultrasound on repair of the Achilles tendons (Tendo
calcaneus) of the rat Cunha A, Parizotto NA, Vidal BC* Ultrasound
in Medicine and Biology - Accepted on August 2001.
*E-mail: vidal@unicamp.b |
| A002-01 RNA relocation
and nucleolus-like bodies persistence at mitosis in benzo[a]pyrene-transformed
human breast epithelial cells after microcell-mediated transfer
of chromosomes 11 and 17 Mello MLS*, Lareef MH, Vidal BC,
Russo J RNA relocation and the incidence of nucleolus-like
bodies during mitosis were investigated in benzo[a]pyrene-transformed
human breast epithelial cells (BP1-E) after microcell-mediated
transfer of normal chromosomes 11 and 17. These chromosomes
have been demonstrated to be involved at early and late transforming
and tumorigenic processes in MCF-10F cells. While RNA relocation
associated with the mitotic spindle did not vary, an increase
in frequency of nucleolus-like bodies resembling the frequency
found in nontransformed cells was found only in BP1-E cells
which received a normal chromosome 11. Some loci retained
in the BP1-E cell nuclei which received this chromosome are
probably involved in the relaxed control of the RNA transcript
production, since only in nontransformed MCF-10F cells there
is an RNA surplus accumulated into nucleolus-like bodies during
cell division. Analytical Cellular Pathology - Accepted on
November 2001. *E-mail: mlsmello@unicamp.br |
A003-01 Effect of sequential
cold shocks on survival and molting rate in Triatoma infestans
Campos SGP, Rodrigues VLCC, Wada CY, Mello MLS* The survival
and molting incidence in moderately fasted and fully-nourished
fifth instar nymphs of Triatoma infestans, a vector of Chagas
disease, were studied following sequential shocks at 0oC.
The shocks were separated by intervals of 8 h and 24 h at
30oC. Cold shock tolerance was demonstrated regardless of
the nutritional state of the insects. Fasting was found to
enhance the tolerance to sequential cold shocks in terms of
molting rate. It was assumed that the cold-tolerance response
presented by T. infestans may have facilitated its successful
survival in areas of South America with seasonally low temperatures.
Memórias do Instituto Oswaldo Cruz - Accepted on December
2001.
*E-mail: mlsmello@unicamp.br |
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A004-01
Comparative analysis of genetic similarity detected by aflp
and coefficient of parentage among cultivars of sugarcane
Lima MLA, Garcia AAF, Oliveira KM, Matsuoka S, Souza Jr.
CL, Souza AP* Despite the economical importance of sugarcane
until the present date no studies have been carried out
to determine the correlation of the molecular-based genetic
similarity (GS) and the coefficient of parentage (f) estimates
generated for commercial cultivars. The objectives of this
study were to (a) assess the level of genetic diversity
among the sugarcane cultivars and (b) investigate the correlation
between the AFLP-based GS and the f, based on pedigree information
of 83 sugarcane genotypes. Amplified fragment length polymorphisms
(AFLP) and pedigree data were used to investigate the genetic
relationship in a group of 3 Saccharum spp. clones and 80
commercial cultivars (interspecific hybrids) used as parents
in breeding programs. GS were determined using Jaccard's
similarity coefficient, and a final dendrogram was constructed
using an unweighted pair-group method with arithmetical
averages (UPGMA). Cluster analysis divided the genotypes
into related subgroups suggesting that there is important
genetic relatedness among the commercial cultivars. AFLP-based
GS and f were significantly correlated, suggesting that
AFLP data may help quantify more accurately the degree of
relatedness among commercial sugarcane cultivars. Theoretical
Applied Genetics
*E-mail: anete@unicamp.br
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A005-01 Ectopic calcification
of articular cartilage in the bullfrog Rana catesbeiana and
its possible involvement in bone closure Felisbino SL, HF
Carvalho* A physiological process of articular cartilage calcification
is described in bullfrogs of different ages. Calcification
of the articular cartilage, detected in animals at 1 month
post-transformation, showed any calcium deposition, began
at a restricted site in the lateral expansion of the cartilage
and then progressed to other areas of the epiphyseal cartilage.
Element analysis showed that calcium crystals consisted of
poorly-crystaline hydroxyapatite. Alkaline phosphatase activity
was detected at the plasma membrane of nearby chondrocytes
and in extracellular matrix. Apoptosis was detected in some
articular chondrocytes from calcified areas. The area occupied
by calcium crystals increased significantly in older animals,
especially in areas under compression. Clusters of needle-like
crystals appeared in the extracellular matrix around the chondrocytes.
In 4-year-old animals, some lamelar bone occurred in the same
area as articular cartilage calcification. The articular cartilage
of R. catesbeiana undergoes precocious and progressive calcification
apparently stimulated by compressive forces. This calcification
is suggested to be involved in the closure of bone extremities,
since calcification appears to precede the formation of a
rudimentary secondary center of ossification in older animals.
Cell Tissue Research
*E-mail: hern@unicamp.br |
A005-01 Ectopic calcification
of articular cartilage in the bullfrog Rana catesbeiana and
its possible involvement in bone closure Felisbino SL, HF
Carvalho* A physiological process of articular cartilage calcification
is described in bullfrogs of different ages. Calcification
of the articular cartilage, detected in animals at 1 month
post-transformation, showed any calcium deposition, began
at a restricted site in the lateral expansion of the cartilage
and then progressed to other areas of the epiphyseal cartilage.
Element analysis showed that calcium crystals consisted of
poorly-crystaline hydroxyapatite. Alkaline phosphatase activity
was detected at the plasma membrane of nearby chondrocytes
and in extracellular matrix. Apoptosis was detected in some
articular chondrocytes from calcified areas. The area occupied
by calcium crystals increased significantly in older animals,
especially in areas under compression. Clusters of needle-like
crystals appeared in the extracellular matrix around the chondrocytes.
In 4-year-old animals, some lamelar bone occurred in the same
area as articular cartilage calcification. The articular cartilage
of R. catesbeiana undergoes precocious and progressive calcification
apparently stimulated by compressive forces. This calcification
is suggested to be involved in the closure of bone extremities,
since calcification appears to precede the formation of a
rudimentary secondary center of ossification in older animals.
Cell Tissue Research
*E-mail: hern@unicamp.br |
A005-01 Ectopic calcification
of articular cartilage in the bullfrog Rana catesbeiana and
its possible involvement in bone closure Felisbino SL, HF
Carvalho* A physiological process of articular cartilage calcification
is described in bullfrogs of different ages. Calcification
of the articular cartilage, detected in animals at 1 month
post-transformation, showed any calcium deposition, began
at a restricted site in the lateral expansion of the cartilage
and then progressed to other areas of the epiphyseal cartilage.
Element analysis showed that calcium crystals consisted of
poorly-crystaline hydroxyapatite. Alkaline phosphatase activity
was detected at the plasma membrane of nearby chondrocytes
and in extracellular matrix. Apoptosis was detected in some
articular chondrocytes from calcified areas. The area occupied
by calcium crystals increased significantly in older animals,
especially in areas under compression. Clusters of needle-like
crystals appeared in the extracellular matrix around the chondrocytes.
In 4-year-old animals, some lamelar bone occurred in the same
area as articular cartilage calcification. The articular cartilage
of R. catesbeiana undergoes precocious and progressive calcification
apparently stimulated by compressive forces. This calcification
is suggested to be involved in the closure of bone extremities,
since calcification appears to precede the formation of a
rudimentary secondary center of ossification in older animals.
Cell Tissue Research
*E-mail: hern@unicamp.br |
A006-01 Sexual dimorphism
in Ramphastos toco and Ramphastos dicolorus (Piciformes, Aves)
Castro MS, Recco-Pimentel SM*, Rocha GT In the family Ramphastidae
there is frequently no apparent phenotypic sexual dimorphism,
except in Pteroglossus viridis and in the genus Selenidera.
Many breeders of wild birds believe that specimens of Ramphastos
toco can be sexed using bill characteristics. In this study,
various discriminant phenotypic variables were analyzed in
birds which were sexed cytogenetically. Fifty-one specimens
of R. toco and 20 R. dicolorus were used. The statistically
significant parameters which served to distinguish the sex
in these species were the length of the culmen and tomium,
length of the lower corneous beak and the cloaca. Using these
parameters, capitive bird breeders can determine sex of R.
toco specimens by phenotypic analysis and form breeding couples
more quickly. Revista de Biologia Tropical
*E-mail: shirlei@unicamp.br |
A007-01 Cytogenetic analysis
of four dart-poison frogs of the Epipedobates genus (Anura,
Dendrobatidae) Aguiar-Jr O, Lima AP, Giaretta AA, Recco-Pimentel
SM* We determined the overall karyotypic structure, positions
of NORs and the distribution of constitutive heterochromatin
for Epipedobates flavopictus, E. trivittatus, E. femoralis,
and E. hahneli. Despite of a conserved chromosome number (2n
= 24), morphological differences were seen in the group of
small chromosomes. E. femoralis presented a distinctive karyotype
compared to the other species analyzed. E. flavopictus is
remarkably different from the others in possessing only centromeric
C-bands. The distribution of heterochromatin varied among
species and seems to evolve as a species-specific trait. NOR
location variability indicates that some rearrangement mechanisms
have taken place during the evolutionary history of this group,
because Dendrobatidae is considered a monophyletic taxon.
Herpetologica.
*E-mail: shirlei@unicamp.br |
A008-01 The biflagellate
spermatozoon of the dart-poison frogs Epipedobates femoralis
and Colostethus sp. (Anura, Dendrobatidae) Aguiar Jr O, Garda
AA, Lima AP, Colli GR, Báo SN, Recco-Pimentel SM* The biflagellate
spermatozoa of the dendrobatids Epipedobates femoralis and
Colostethus sp. were studied using light and transmission
electron microscopy. Each flagellum is formed by an axial
fiber connected to the axoneme by an axial sheath; juxta-axonemal
fibers are absent. This is the second true case of biflagellarity
reported in anurans. The biflagellar condition is tentatively
considered a plesiomorphic trait within the Dendrobatidae.
Since E. femoralis may be more related to some species of
Colostethus than to other congeneric species, we suggest that
Colostethus is paraphyletic. Journal of Morphology
* E-mail: shirlei@unicamp.br |
A009-01 Optical anisotropy
of a pig tendon under compression Feitosa VLC, Vidal BC, Pimentel
ER* The distribution and organization of collagen bundles
and proteoglycans in two portions of the proximal region of
the superficial digital flexor tendon of pigs were studied
under polarized light microscopy. The strong metachromasy
observed in the sp portion, which is in direct contact with
the bone, after toluidine blue staining demonstrated the presence
of proteoglycan in a compression-bearing region. A higher
molecular order of the collagen bundles as well as a more
regular pattern of crimp in areas under tension, like in the
dp region, probably attend a major requirement for tendon
stretching. Linear dichroism confirmed that the glycosaminoglycans
were disposed parallel to the longest axis of the collagen
bundles. Journal of Anatomy
*E-mail: pimentel@unicamp.br |
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