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Instituto de Biologia Unicamp
Abstracta 2003








A001-03 Cell death evaluation in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17
Mello MLS*, Barbisan LF, Lareef MH, Russo J, Vidal BC
The incidence of apoptosis and nuclear instability (including catastrophic death) were studied in benzo[a]pyrene (BP)-transformed human breast epithelial cells (BP1-E cell line) after microcell-mediated transfer of normal chromosomes 11 and 17, on expecting that the cell death rates on tumorigenic cells would be affected by this treatment. The transfer especially of the chromosome 17, to BP1-E cells, increased the apoptotic ratios and decreased the nuclear instability ratios, thus showing that the microsatellite instability and loss of heterozygosity induced by BP in these chromosomes of the non-transformed human breast epithelial cells affect the control of cell death mechanisms.
Mutation Research (Fundamental and Molecular Mechanisms of Mutagenesis) 546(1-2) – Accepted on October 2003

A002-03 Eye pigments of the blood-sucking insect, Triatoma infestans (Hemiptera, Reduviidae)
Moraes AS, Pimentel ER, Rodrigues VLCC, Mello MLS*
Pigments from black (wild) and red (mutant) eyes of Triatoma infestans were studied spectrophotometrically and compared with those from red-eyed (wild) and white-eyed (mutant) Drosophila melanogaster. The spectral absorption profiles and ascending paper chromatographic results indicated that both eye forms of T. infestans contained ommochromes of the xanthommatin type. The eye color difference in T. infestans was considered to depend on the xanthommatin concentration, with a smaller content of this ommochrome in red eyes.
Brazilian Journal of Biology 64 – Accepted on October 2003

A003-03 Feulgen staining in Malpighian tubules of meliponid bees: a methodological contribution
Mampumbu AR, Vidal BC, Mello MLS*
Brazilian Journal of morphological Science – Accepted on August 2003

A004-03 Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology
Beletti ME, Mello MLS*
Toluidine blue staining and response to the Feulgen reaction were compared in sperm cells from rabbits with unknown fertility in order to assess chromatin alteration. The relationship between sperm morphology and chromatin alteration was also investigated. Seven hundred ejaculates were examined. The results indicated that sperm chromatin structure affected sperm head morphology. The toluidine blue staining was found to be more appropriate to characterize the intensity of chromatin alteration. When the final chromatin compaction which occurs in the epididymus was affected, secondary sperm defects could also be detected.
Theriogenology – Accepted on October 2003

A005-03 Seasonal changes in testicular and epididimal histology of the tropical lizard, Tropidurus itambere, during its annual productive cycle
Ferreira A, Dolder H*

A006-3 Sperm ultrastructure and spermatogenesis in the lizard, Tropidurus itambere
Ferreira A, Dolder H*

A007-03 Cytochemical study of the spermiogenesis and mature spermatozoon of the lizard, Tropidurus itambere, (Reptilia: Squamata)
Ferreira A, Dolder H*
Acta Histochemica

A008-03 Dichotomic spermiogenesis of Euptoieta hegesia Lepidoptera: Nymphalidae)
Mancini K, Dolder H*
Brazilian Journal of Morphological Sciences

A009-03 Structure and ultrastructure of spermatozoa in Meliponini (Stingless bees) (Hymenoptera: Apidae)
Zama U, Lino-Neto J, Dolder H*
Tissue & Cell

A010-03 Observations on the feeding of the Cirratulid Cirriformia filigera
Pardo EV, Amaral ACZ*
Observations on the feeding behavior of Cirriformia filigera (delle Chiaje, 1825) (Annelida: Polychaeta) from the intertidal region of São Francisco and Engenho D’água beaches (São Sebastião, state of São Paulo) were made in the laboratory. This species, like other cirratulids, is a deposit feeder, feeding mainly on the surface of the sediment with the aid of its grooved and ciliated palps, wich are employed to capture food particles. The collected food particles pass slightly over the groove of each palp and go straight to the mouth. Cirriformia filigera lies just beneath the surface of the substrate, in a J-shaped tube. It extends about 4 branchial filaments into the overlying water for aeration. When the worms move with the prostomium sideways, they collect and transport sand grains backwards. These grains pass along the ventral region of the animal until they reach the middle part of its body. At this point, the palps and branchial filaments take over, moving the sand grains to the posterior end of the dorsal side of the animal, covering this area with them. Some sand grains were also seen to be ingested during these movements.
Brazilian Journal of Biology 64(3)

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