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A001-03 Cell death evaluation in benzo[a]pyrene-transformed
human breast epithelial cells after microcell-mediated transfer of chromosomes
11 and 17
Mello MLS*, Barbisan LF, Lareef MH, Russo J, Vidal BC
The incidence of apoptosis and nuclear instability (including catastrophic
death) were studied in benzo[a]pyrene (BP)-transformed human breast
epithelial cells (BP1-E cell line) after microcell-mediated transfer
of normal chromosomes 11 and 17, on expecting that the cell death rates
on tumorigenic cells would be affected by this treatment. The transfer
especially of the chromosome 17, to BP1-E cells, increased the apoptotic
ratios and decreased the nuclear instability ratios, thus showing that
the microsatellite instability and loss of heterozygosity induced by
BP in these chromosomes of the non-transformed human breast epithelial
cells affect the control of cell death mechanisms.
Mutation Research (Fundamental and Molecular Mechanisms of Mutagenesis)
546(1-2) – Accepted on October 2003
*E-mail: mlsmello@unicamp.br
A002-03 Eye pigments of the blood-sucking insect,
Triatoma infestans (Hemiptera, Reduviidae)
Moraes AS, Pimentel ER, Rodrigues VLCC, Mello MLS*
Pigments from black (wild) and red (mutant) eyes of Triatoma infestans
were studied spectrophotometrically and compared with those from red-eyed
(wild) and white-eyed (mutant) Drosophila melanogaster. The spectral
absorption profiles and ascending paper chromatographic results indicated
that both eye forms of T. infestans contained ommochromes of the xanthommatin
type. The eye color difference in T. infestans was considered to depend
on the xanthommatin concentration, with a smaller content of this ommochrome
in red eyes.
Brazilian Journal of Biology 64 – Accepted on October 2003
*E-mail: mlsmello@unicamp.br
A003-03 Feulgen staining in Malpighian tubules of
meliponid bees: a methodological contribution
Mampumbu AR, Vidal BC, Mello MLS*
Brazilian Journal of morphological Science – Accepted on August
2003
*E-mail: mlsmello@unicamp.br
A004-03 Comparison between the toluidine blue stain
and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation
and their relationship with sperm morphology
Beletti ME, Mello MLS*
Toluidine blue staining and response to the Feulgen reaction were compared
in sperm cells from rabbits with unknown fertility in order to assess
chromatin alteration. The relationship between sperm morphology and
chromatin alteration was also investigated. Seven hundred ejaculates
were examined. The results indicated that sperm chromatin structure
affected sperm head morphology. The toluidine blue staining was found
to be more appropriate to characterize the intensity of chromatin alteration.
When the final chromatin compaction which occurs in the epididymus was
affected, secondary sperm defects could also be detected.
Theriogenology – Accepted on October 2003
*E-mail: mlsmello@unicamp.br
A005-03 Seasonal changes in testicular and epididimal
histology of the tropical lizard, Tropidurus itambere, during its annual
productive cycle
Ferreira A, Dolder H*
Zoocriadeiros
*E-mail: heidi@unicamp.br
A006-3 Sperm ultrastructure and spermatogenesis in
the lizard, Tropidurus itambere
Ferreira A, Dolder H*
Biocell
*E-mail: heidi@unicamp.br
A007-03 Cytochemical study of the spermiogenesis and
mature spermatozoon of the lizard, Tropidurus itambere, (Reptilia: Squamata)
Ferreira A, Dolder H*
Acta Histochemica
*E-mail: heidi@unicamp.br
A008-03 Dichotomic spermiogenesis of Euptoieta hegesia
Lepidoptera: Nymphalidae)
Mancini K, Dolder H*
Brazilian Journal of Morphological Sciences
*E-mail: heidi@unicamp.br
A009-03 Structure and ultrastructure of spermatozoa
in Meliponini (Stingless bees) (Hymenoptera: Apidae)
Zama U, Lino-Neto J, Dolder H*
Tissue & Cell
*E-mail: heidi@unicamp.br
A010-03 Observations on the feeding of the Cirratulid
Cirriformia filigera
Pardo EV, Amaral ACZ*
Observations on the feeding behavior of Cirriformia filigera (delle
Chiaje, 1825) (Annelida: Polychaeta) from the intertidal region of São
Francisco and Engenho D’água beaches (São Sebastião,
state of São Paulo) were made in the laboratory. This species,
like other cirratulids, is a deposit feeder, feeding mainly on the surface
of the sediment with the aid of its grooved and ciliated palps, wich
are employed to capture food particles. The collected food particles
pass slightly over the groove of each palp and go straight to the mouth.
Cirriformia filigera lies just beneath the surface of the substrate,
in a J-shaped tube. It extends about 4 branchial filaments into the
overlying water for aeration. When the worms move with the prostomium
sideways, they collect and transport sand grains backwards. These grains
pass along the ventral region of the animal until they reach the middle
part of its body. At this point, the palps and branchial filaments take
over, moving the sand grains to the posterior end of the dorsal side
of the animal, covering this area with them. Some sand grains were also
seen to be ingested during these movements.
Brazilian Journal of Biology 64(3)
*E-mail: ceamaral@unicamp.br
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