Abstracta 2004

2005

ARTIGOS ACEITOS PARA PUBLICAÇÃO

A001-04 Extrafloral nectary-mediated ant-plant interactions in the coastal vegetation of Veracruz, Mexico: Richness, occurrence, seasonality and ant foraging patterns

Díaz-Castelazo C, Rico-Gray V, Oliveira PS*, Cuautle M

We studied the extrafloral nectary-mediated ant-plant interactions in coastal Veracruz, Mexico. Forty-three species had EFNs, and 66% of these were visited by ants. Thirty-one ant species were found in 208 associations with EFNs. Richness and species composition of ants at EFNs differed between day and night, while ant abundance did not. Abundance of nectary-bearing plants and ant species richness did not differ among vegetation types, while ant abundance and density did. The high proportion of EFN-bearing plants (14.82%) compared to other rate habitats, suggests that EFN-mediated plant protection by ants is important in coastal Veracruz.

Ecoscience

*E-mail: pso@unicamp.br

A002-04 Seed removal by ants from faeces produced by different vertebrate species

Pizo MA, Guimarães Jr PR, Oliveira PS*

Seed removal by ants from vertebrate faeces is influenced by the seed species involved. In this study experimental seed-containing faecal portions were prepared using defecations of birds, marsupials, and monkeys. Seed species and type of faeces affected the proportion of seeds removed. The results indicate that seed species affects seed removal by ants, while the type of faeces probably interacts with seed traits to influence faeces detection.

Ecoscience

*E-mail: pso@unicamp.br

A003-04 Host-Plant Flowering Status and Sugar Concentration of Phloem Sap: Effects on an Ant-Treehopper Interaction

Quental TB, Trigo JR, Oliveira PS*

Host-plant mediation of ant-hemipteran interaction was tested. Total N content of the phloem did not differ significantly for plants with or without flowers, whereas sugar concentration was higher in plants with flowers. Honeydew sugar concentration and production did not vary with flowering. Plant flowering status had no effect on ant-derived protection to treehoppers, but plants with flowers accumulated more natural enemies through time than plants without flowers. It is suggested that a trade-off between feeding on a higher-quality phloem and being at predation-prone flowering individuals may underlie this ant-hemipteran interaction.

European Journal of Entomology

*E-mail: pso@unicamp.br

A004-04 Faecal shield of the tortoise beetle Plagiometriona flavescens (Chrysomelidae: Cassidinae) as chemically mediated defence against predators

Nogueira-de-Sá F, Trigo JR*

Larvae of Plagiometriona aff. flavescens carry a structure on their back made of faeces and exuviae, called a faecal shield, which may protect larvae against natural enemies. To test if chemicals found in the faecal shield of Plagiometriona aff. flavescens provided defence for larvae, experiments in the field and in the laboratory (using the ant Camponotus crassus, and chicks Gallus gallus as model predators) were undertaken. Both field and laboratory experiments showed that live larvae with faecal shields, as well as baits treated with faecal shield extracts, were rejected by predators, confirming the chemical nature of this kind of defence.

Journal of Tropical Ecology 21: 1-6, 2005.

*E-mail: trigo@unicamp.br

A005-04 Sistemas de liberação controlada com bupivacaína racêmica (S50-R50) e mistura enantiomérica de bupivacaína (S75-R25): efeitos da complexação com ciclodextrinas no bloqueio do nervo ciático em camundongos

Braga AFA, Araújo DR, Fraceto LF, de Paula E*

Efeitos tóxicos da bupivacaína levaram à procura por novos anestésicos locais (AL), levando ao desenvolvimento das preparações enantioméricas (bupivacaína S75-R25). A complexação de AL menos tóxicos que a bupivacaína é de grande interesse no desenvolvimento de formulações mais seguras. Neste estudo avaliamos a eficácia anestésica de complexos da mistura enantiomérica bupivacaína S75-R25 e da bupivacaína racêmica (S50-R50) com hidroxipropil-CD comparando-os com soluções comerciais. Os bloqueios motor e sensorial induzidos pelas drogas livres e complexadas foram avaliados em concentrações de AL: 0,125; 0,25 e 0,5 %. A complexação: i) reduziu a latência sem modificar a intensidade do bloqueio motor dos complexos S75-R25 e S50-R50, em relação às drogas livres; ii) levou a um aumento na intensidade/duração da analgesia para S75-R25 e S50-R50, quando comparados aos AL comerciais. Os efeitos analgésicos mais pronunciados obtidos com a complexação com CD mostram que ambas formulações são de interesse para alívio da dor pós-operatória, podendo ser administradas em menores concentrações. A mistura enantiomérica S75-R25, por ser menos tóxica, representa uma alternativa de formulação de liberação controlada mais segura e eficaz.

Revista Brasileira de Anestesiologia

*E-mail: depaula@unicamp.br

A006-04 Determining the Location of Local Anesthetics Inside Lecithin Bilayers by Longitudinal Relaxation Times – A Single Method

Fraceto LF, Spisni A, Schreier S, de Paula E*

In this work we collected evidence for the specific location of the aminoamide local anesthetics (LA) lidocaine, etidocaine, bupivacaine and mepivacaine in sonicated vesicles of egg phosphatidylcholine, EPC. NMR is a potent tool for the detection of putative preferential location of amphiphilic molecules inside membranes. Changes in Chemical Shift and longitudinal relaxation times, T₁, allowed the determination of changes in the moiety/mobility of EPC with LA. We propose that the preferential positioning of LA also takes place in the axons, where it modulates the access of the LA to their sites of action in the voltage-gated sodium channel.

Biophysical Chemistry - Accepted on December 2004

*E-mail: depaula@unicamp.br

A007-04 Immunochemical detection of Lonomia obliqua caterpillar venom in rats

Da Silva GH, Panunto PC, Hyslop S, Cruz-Höfling MA*

Microcopy Research Technique

*E-mail: hofling@unicamp.br

A008-04 Effect of neutrophil depletion in the local pathological alterations and muscle degeneration in mice injected with Bothrops jararaca snake venom

Teixeira CFP, Chaves F, Zamunér SR, Zuliani JP, Fernandes CM, Cruz-Höfling MA*, Fernandes I, Gutiérrez JM

International Journal of Experimental Pathology

*E-mail: hofling@unicamp.br

A009-04 Rabbit antiserum against non-toxic submit of crotoxin partially neutralize its neurotoxicity but not inhibit the enzymatic activity and the miotoxicity

Beghini DG, Cruz-Höfling MA*, Rodrigues-Simioni L, Delatorre MC, Novello JC, Hyslop S, Marangoni S

Toxicon

*E-mail: hofling@unicamp.br

A010-04 Programmed cell death in thymus during experimental paracoccidioidomycosis

Souto PCS, Brito VN, Gameiro J, Cruz-Höfling MA*, Verinaud L

Medical Microbiology and Immunology

*E-mail: hofling@unicamp.br

A011-04 A new species of Ophiomisidium (Echinodermata: Ophiuroidea) from the continental shelf and slope off southern Brazil

Borges M, Monteiro AMG, Amaral, ACZ*

A new species of Ophiomisidium is described and information is presented on size-related changes in its morphology. It is the first of the eight known species of Ophiomisidium to be recognized as having brooding development. Over 1,000 specimens of the new species were collected on the continental shelf and slope (60-810 m depth) off southern Brazil. They were obtained during the REVIZEE Program, the first extensive surveys to examine Brazilian ophiuroids from depths greater than 200 m.

Zootaxa

*E-mail: ceamaral@unicamp.br

A012-04 Structure of molluscan assemblages in sheltered intertidal unconsolidated environments.

Denadai MR, Amaral ACZ*, Turra A

The molluscan macrofauna from 13 oceanic sheltered intertidal unconsolidated environments and its relationship with abiotic factors were studied in order to establish the degree of species richness and to understand the role environment plays in structuring such assemblages. Four distinct intertidal habitat types were recognized based on molluscan assemblage descriptors and abiotic characteristics. The mean grain size (in phy units) and the beach slope showed a negative relationship with the diversity, richness and density. Coarser sediments are favorable to molluscan fauna in the study areas, contrasting the well-known negative effect of this type of sand on fauna in typical oceanic beaches. The low-tide terraces, typical from tide-dominated areas, and the presence of rocky fragments and gravel structures were also associated to the higher values of richness. The high richness in the study area as a whole seems to be a direct consequence of its environmental heterogeneity, once it is composed by quite distinct habitat types.

Brazilian Archives of Biology and Technology

*E-mail: ceamaral@unicamp.br

A013-04 Along and across-shore components of the spatial distribution of the clam Tivela mactroides (Born, 1778) (Bivalvia, Venereidae)

Denadai MR, Amaral ACZ*, Turra A

Fishery of beach clams has been viewed as an income alternative to artisanal fisherman that is highly vulnerable to urbanization of coastal areas. Tivela mactroides is a beach clam commonly harvested in the Caribbean (Venezuela) and in south-eastern Brazilian coast that has only localized records on its spatial structure in size and abundance to subside management purposes. The present study revealed that the spatial distribution in abundance and size of T. mactroides in Caraguatatuba Bay, SP, Brazil, presents both along and across-shore (intertidal and subtidal) components, fact that should be taken into account in the design of further population or monitoring studies concerning this species. The intertidal and subtidal across-shore distribution of clam abundance and size was shown to vary between and within areas (Southern and Northern). The individuals from the southern area were concentrated in intermediate tidal levels (0.2 to 0.4 m in relation to mean low water) while in the northern they were more homogeneously distributed. The highest limit of the distribution of this species was 0.8 m (southern) and 0.4 m (northern), and an upward size increase tendency was more evident in the northern area. In the subtidal, the abundance and individual size tended to increase from the deepest (4.5 m) to the shallowest (0.5 m) levels only in the southern area. An ontogenic distribution was recorded for this species, with small-sized individuals being recorded only in the subtidal. Possible causes for the patterns recorded and for differences between areas are discussed and specific studies are suggested to formally evaluate the proposed hypotheses.

Journal of Natural History

*E-mail: ceamaral@unicamp.br

A014-04 Morphometric analyses of two species of Scolelepis (Polychaeta: Spionidae)

Maccord FS, Amaral ACZ*

Prior to studying the biology of two species of Scolelepis, it was necessary to define the structure that would serve as the best indicator of age (size) classes. Seven hundred and six specimens of Scolelepis cf. chilensis (Hartmann-Schröder, 1962) and 551 specimens of Scolelepis goodbodyi (Jones, 1962) were analyzed. Individuals of each species were classified according to sex. The width (W) and height (h) of setigers 3 and 5, the position of the first and the last gametogenic setigers, the total number of setigers, and the total length of each worm were measured. The area and volume of the setigers were estimated. Discriminant analysis was used to determine whether there were sexually dimorphic features, apart from the presence of gametes. Linear regression analysis was used to evaluate the relationship between each parameter and the number of setigers. S. cf. chilensis was significantly larger than S. goodbodyi. These species also differed in the position of the first gametogenic setiger, which averaged 27 (sd = 3) in S. cf. chilensis and 22 (sd = 1) in S. goodbodyi. There were no sexually dimorphic features in either sex of S. cf. chilensis or S. goodbodyi. The total length showed the strongest correlation with the number of setigers in both species, followed by the width and the volume of setigers.

Journal of the Marine Biological Association of the United Kingdom

*E-mail: ceamaral@unicamp.br

A015-04 Chromatin supraorganization, DNA fragmentation, and cell death in snake erythrocytes

Miyamoto M, Vidal BC, Mello MLS*

Chromatin supraorganization, DNA fragmentation and cell death were investigated in four snake species (Crotalus durissus terrificus, Bothroups jararaca, B. alternatus, and B. neuwiedii), which differ in their geographical distribution and habitats, by using image analysis of Feulgen hydrolysis kinetics, the TUNEL assay, single-cell gel electrophoresis, and transmission electron microscopy. The differences found in the erythrocyte biology of the mentioned snakes are assumed to be related to selected hemoglobin variants, and that could lead the cells to different resistances to unfavorable environmental conditions.

Biochemistry and Cell Biology - Accepted on September 2004.

*E-mail: mlsmello@unicamp.br

A016-04 Chromatin supraorganization and extensibility in mouse hepatocytes following starvation and refeeding

Moraes AS, Vidal BC, Guaraldo AMA, Mello MLS*

The effect of 48 h of starvation and of 48 h of refeeding following starvation on chromatin supraorganization and extensibility (formation of extended chromatin fibers) was studied in hepatocytes from adult mice, using topochemical tests, image analysis, gravity action, and polarization microscopy. The results indicated loss, regain, and redistribution of nuclear proteins (nuclear matrix) related to chromatin organization and extensibility depending on the nutritional state of the organism.

Cytometry A – Accepted on November 2004.

*E-mail: mlsmello@unicamp.br

A017-04 Riboflavina: uma vitamina multifuncional

Souza ACS, Ferreira CV, Jucá MB, Aoyama H*, Cavagis ADM, Peppelenbosch MP

Riboflavin plays important roles in biochemistry, specially in redox reactions, acting as photosensitizer It promotes hydroperoxides reduction but also contributes to oxidative stress producing superoxide. Low riboflavin intakes have been associated to different diseases, such as cancer and cardiovascular diseases. This multifunctional roles allows riboflavin to be considered a versatile and important biological compound.

Química Nova

*E-mail: secrbioq@unicamp.br

A018-04 Anatomic characteristics of the double lip: a case related

Caldeira EJ, Fávaro WJ, Minatel E, Camilli JA, Cagnon VHA*

The double lip causes psychological embarrassment to its bearer despite of being characterized as a benign alteration. In the anamnesis, a 32 years-old man said, that five years ago he has unusual infolding in the superior lip. The lip volume increasing is not related to traumatism or parafunctional habits. The treatment was mode by surgical excision of the surplus tissue with esthetic and functional correction.

Revista Colombiana de Odontología – Accepted on August 2004

*E-mail: quitete@unicamp.br

A019-04 Morphometric analysis and ultrastructure of the oral mucosa of diabetic NOD mice

Caldeira EJ, Garcia PJ, Minatel E, Camilli JA, Cagnon VHA*

The present study aims to perform histological, ultrastructural and stereological analyses of the oral mucosa in spontaneously diabetic mice and correlate the findings with the processes of tissue repair, healing and susceptibility to infection. After characterization of diabetic status, samples were collected from the oral mucosa and processed for light and transmission electron microscopy. Reduced epithelial thickness and atypical epithelial cells mainly in the basal cell layer, accompained by a marked involution of cell volume, were observed in the diabetic group. It was concluded that spontaneous diabetes leads to changes in the basic biology of the oral mucosa, compromissing tissue function and favoring the occurrence of oral infections.

Brazilian Journal of Morphological Sciences

*E-mail: quitete@unicamp.br

A020-04 Cryopreserved muscle basal lamina grafts retain their grafting potential for nerve repair

Santo Neto H*, Sabha Júnior MJJ, Marques MJ*

We examined the effect of cryopreservation on the ability of muscle basal lamina grafts to repair nerve lesions. Their grafting potential was evaluated by examining axonal regeneration after autografting to lesions in rat sciatic nerves. Muscle basal lamina grafts cryopreserved for up to 30 weeks at -20°C and -40°C were successfully used. No significant differences in the parameters of axonal regeneration between cryopreserved and non-cryopreserved grafts were found. In conclusion, muscle basal lamina autografts retain their potential usefulness for nerve repair after cryopreservation, providing a basis for the development of a bioprosthesis from muscle basal lamina.

Cryobiology- Accepted on October 2004.

*E-mail: marques@unicamp.br

A021-04 The use of hydroxyapatite and autogenous cancellous bone grafts to repair bone defects in rats

Silva RV, Camilli JÁ*, Bertran CA, Moreira NH.

The objective of this study was to evaluate the repair of bone defects by autogenous cancellous bone grafts or porous bioceramic discs of hydroxyapatite/phosphate cement mixture. Two 5-mm diameter defects were made in the skulls of rats and filled with the bioceramic material or cancellous bone. The rats were sacrificed 2, 4, 8 and 24 weeks after surgery and tissue samples were analyzed by radiography and histology. By the 24^th week, the defects filled with autogenous cancellous bone grafts or bioceramic material showed similar volumes of bone tissue within the defect. However, defects treated with bioceramic material were almost completely closed as a result of the joining of ceramic fragments and the neoformed bone tissue, while those filled with autogenous grafts showed several areas filled with connective tissue. These results indicated that the osteointegration of bioceramic fragments allowed the reconstruction of parietal bone defects without the need for a bone graft.

International Journal of Oral Maxillary Surgery – Accepted on June 2004

*Phone: 19-3788-6101

A022-04 Sugarcane yellow leaf virus (ScYLV) Infection Leads to Reduced Photosynthetic Efficiency, Increased Susceptibility to Photoinhibition and Carbohydrate Accumulation in Sugarcane Leaves

Gonçalves MC, Vega J*, Oliveira JG, Gomes MMA.

Fitopatologia Brasileira

*E-mail: jvega@unicamp.br

A023-04 Ritmos hormonais do pâncreas endócrino: dos fundamentos cronobiológicos às implicações clínicas

Delattre E*

Inicialmente, são apresentadas as bases teóricas para o conhecimento dos ritmos endócrinos, considerando-se que, virtualmente, todas as secreções hormonais apresentam acentuadas oscilações, o que demonstra um alto grau de organização temporal do sistema endócrino. A seguir, o trabalho revisa a ritmicidade do pâncreas endócrino, ressaltando os ritmos circadianos e ultradianos da insulina, em razão de sua importância para a fisiologia e fisiopatologia desse órgão. São apresentados tanto os resultados obtidos in vitro, quanto aqueles verificados clinicamente. Discutem-se as razões biológicas para a secreção pulsátil dos hormônios em geral, bem como as alterações desse ritmo em patologias do pâncreas endócrino.

Medicina, Ribeirão Preto 37

*E-mail: delattre@unicamp.br

A024-04 Preliminary studies of Mammea americana L. (Guttiferae) bark/latex extract, points out to an effective antiulcer effect on gastric ulcer models in rats

Toma W, Hiruma-Lima CA, Souza Brito ARM*

Phytomedicine

*E-mail: abrito@unicamp.br

A025-04 Is gastric ulceration different in normal and malnourished rats?

Paula ACB, Gracioso JS, Toma W, Bezerra R, Saad MAJ, De Lucca IMS, Carneiro EM, Souza Brito ARM*

British Journal of Nutrition

*E-mail: abrito@unicamp.br

A026-04 Flavonoids and preliminary studies of the antiulcerogenic activity from Byrsonima crassa Niedenzu (IK) (Malpighiaceae) leave extracts

Sannomiya M, Rodrigues CM, Coelho RG, dos Santos LC, Hiruma-Lima CA, Souza Brito AR*, Vilegas W

Journal of Ethnopharmacology

*E-mail: abrito@unicamp.br

A027-04 Evaluation of Strychnos pseudoquina St. Hil. leaves extract on gastrointestinal activity in mice

Silva MA, Hiruma-Lima CA,. Soares VC, Raddi MSG, Souza-Brito ARM*, Vilegas W

Zeitschrift für Naturforschung

*E-mail: abrito@unicamp.br

A028-04 Evolutionary and Structural Analysis of the Cytochrome C Oxidase Subunit I (COI) Gene from Haematobia irritans, Stomoxys calcitrans and Musca domestica (Diptera: Muscidae) Mitochondrial DNA

Oliveira MT, Azeredo-Espin AML*, Lessinger AC

The mtDNA COI gene was characterized for three economically important dipterans: Haematobia irritans, Stomoxys calcitrans and Musca domestica. The nucleotide bias for an A+T-rich sequence is linked to features: high A+T throughout the entire gene, high A+T in the third codon position, and predominance of A+T-rich codons. A TCG (serine) start codon was identified. Comparisons to other dipteran species showed high levels of sequence conservation. Analysis of divergent aminoacids and COI protein topologies agreed with the evolutionary model of insect COI protein. The characterization of structure and evolution of this gene could be informative for further evolutionary analysis of dipteran species.

DNA Sequence – Accepted on December 2004.

E-mail: azeredo@unicamp.br

A029-04 Genetic approaches for studying myiasis-causing flies: molecular markers and mitochondrial genomics

Azeredo-Espin AML*, Lessinger AC

Myiasis-causing flies can be divided generally into saprophagous (free-living species), facultative or obligate parasites Many of these flies are of great medical and veterinary importance in Brazil, because of their role as livestock key-insect-pests and vectors of pathogens besides their recognition as important legal evidences in forensic entomology. Characterization of molecular markers in the mtDNA and nuclear DNA of myiasis-causing flies have been efficiently used for identification of evolutionary mechanisms responsible for specific patterns of genetic variability and were useful for the analysis of population structures of the New World screwworm fly (NWS) Cochliomyia hominivorax and bot fly Dermatobia hominis. Different aspects regarding organization, evolution and potential applications of the mitochondrial genome and the analysis of nuclear markers for population genetic studies in myiasis-causing flies from Brazil were discussed in this review.

Genetica – Accepted on December 2004.

*E-mail: Azeredo@unicamp.br

A030-04 Homogeneity Test Of Hardy-Weinberg Deviations In Brazilian Littorinids: Evidence For Selection?

Andrade SCS, Medeiros HF, Solferini VN*

The aim of this study was to evaluate the occurrence of Hardy-Weinberg disequilibrium and to verify if there is a pattern in deviations in populations of Echinolittorina lineolata, Littoraria flava and Littoraria angulifera. Isozyme analyses were done on a total of 39 populations of these species, collected along 4,000 km of the Brazilian coast. Most populations showed heterozygote deficiency in several loci. A homogeneity test among loci showed heterogeneous F_IS values in most populations. All littorinid species showed homogeneous F_IS values at PGM locus. This finding suggest that this enzyme could have an important role in the fitness of these periwinkles.

Journal of Molluscan Studies

*E-mail: solferin@unicamp.br

A031-04 Sesquiterpene and polyacetylene profile of Bidens pilosa complex (Asteraceae: Heliantheae) from Southeast of Brazil

Gromboni-Guarantini MT, Silva-Brandão KL, Solferini VN, Semir J, Trigo JR*

Bidens pilosa is a species with several taxonomic problems. Recently it has been shown to be a complex of different species. Dicloromethane extract of leaves of several populations of three proposed species for the complex in southeastern Brazil (B. pilosa, B. alba and B. subalternans) were analyzed by GC-MS. Twenty-four substances were detected and five sesquiterpenes tentatively identified as E-caryophyllene, -humulene, germacrene D, bicyclogermacrene and -muurolene were found in all three Bidens species. The polyacetylene phenylhepta-1,3,5-triyne was identified only in B. alba. Multivariate analysis separated the three entities, suggesting that these compounds could represent a useful tool to distinguish species in the B. pilosa complex.

Biochemical Systematics and Ecology

*E-mail: trigo@unicamp.br

A032–04 Biochemical changes during the development of Witche’s Broom: the most important disease of cocoa in Brazil caused by Crinipellis perniciosa

Scarpari LM, Meinhardt LW, Mazzafera P, Pomella AWV, Schiavinato MA, Cascardo JCM, Pereira GAG*

Vassoura de bruxa, causada por Crinipellis perniciosa, é a principal doença do cacaueiro (Theobroma cacao) no hemisfério sul. Neste estudo, analisaram-se vários compostos em plântulas durante o desenvolvimento da doença. Alterações foram observadas no conteúdo de açúcares solúveis (sacarose, glicose e frutose), asparagina, alcalóides (cafeína e teobromina), etileno e taninos. Etileno e taninos aumentaram antes do desenvolvimento dos sintomas e declinaram após a morte dos tecidos. Além disso, as concentrações de MDA, glicerol e a composição de ácidos graxos sofreram alterações nos tecidos infectados. Estes resultados mostram alterações bioquímicas coordenadas, sugerindo que o etileno desempenha papel chave no desenvolvimento da doença.

Journal of Experimental Botany – Accepted on July, 2004.

*E-mail: goncalo@unicamp.br

A033-04 Extracellular matrix of ostrich articular cartilage

Tomiosso TC, Gomes L, Vidal BC, Pimentel ER*

Biocell – Accepted on December 2004.

*E-mail: pimentel@unicamp.br

A034-04 Immature stages of Napeogenes sulphurina Bates, 1862 (Nymphalidae, Ithomiinae) from Northeastern Brazil

Freitas AVL*, Brown Jr KS

O trabalho descreve a planta hospedeira e os estágios imaturos de Napeogenes sulphurina da região Nordeste do Brasil (Alagoas e Pernambuco), comparando seus caracteres morfológicos e ecológicos com o de outras espécies da tribo Napeogenini (Ithomiinae). As larvas são isoladas, com cápsula preta nos primeiros estádios e com faixa preta frontal nos últimos estádios. A coloração geral é cinza escura, com uma faixa lateral amarela clara ao longo das protuberâncias sublaterais. As pupas são compactas, de cor geral bege, com padrão geral prateado após os primeiros dias.

Journal of the Lepidopterists’ Society

*E-mail: baku@unicamp.br

A035-04 Immature stages of Ithomia arduinna (Lepidoptera: Nymphalidae, Ithomiinae)

Freitas AVL*, Brown Jr KS

The host plant and the immature stages of Ithomia arduinna are described. The isolated eggs are laid under mature leaves of Aureliana sp. (Solanaceae). The larvae pass through five instars, the last with a conspicuous ringed pattern; the pupae are beige and reflective, very similar to those of other species of Ithomia. The color-pattern of the mature larva of this species is very different from those of the other known species of the genus, suggesting that much additional work on Ithomiinae juveniles should be done before patterns can be generalized.

Tropical Lepidoptera

*E-mail: baku@unicamp.br

A036-04 Description of the early stages of Leucidia (Lepidoptera, Pieridae).

Freitas AVL*

The host plant and the immature stages of Leucidia brephos and Leucidia elvina are described. The isolated eggs are laid under mature leaves of Senna spp. (Leguminosae). The larvae pass through four instars, with the last being somewhat different in the two species studied; the pupae are green, narrow and elongated, similar to those of Phoebis and Eurema. The immatures of Leucidia show that this genus should be placed within the Coliadinae.

Tropical Lepidoptera

*E-mail: baku@unicamp.br

A037-04 Conservation of terrestrial invertebrates and their habitats in Brazil

Lewinsohn TM*, Freitas AVL, Prado PIK

As one of the prime megadiverse countries of the world, Brazil holds an immense number of terrestrial invertebrates. Current knowledge of this biota is very heterogeneous. The current Brazilian national and regional Red Lists include 130 terrestrial invertebrate species, of which 42% are butterflies. These lists are thus contingent on available knowledge, so that many unmentioned taxa certainly include species at risk. Knowledge of various biomes and habitats is also quite irregular, with the Caatinga and Pantanal in need of more study, compared to the Atlantic Forest, Amazon and Cerrado biomes. Canopy, host-associated and soil faunas also need further intensive study. Invertebrate conservation will be more effectively promoted by habitat preservation and management rather than single-species initiatives. Better geographic surveys of entire taxonomic or functional assemblages are needed. Likewise, better understanding of ecosystem processes that depend on invertebrates will certainly strenghten the case for their conservation.

Conservation Biology

*E-mail: thomasl@unicamp.br

A038-04 Immunocytological localization of tubulins in spermatids and spermatozoa of Euptoieta hegesia (Lepidoptera: Nymphalidae)

Mancini K, Dolder H*

Tissue and Cell

*E-mail: heidi@unicamp.br

A039-04 Ultrastructural characterization of spermatozoa in Euglossini bees (Hymenoptera: Apidae: Apinae)

Zama U, Lino Neto J, Mello SM, Campos LAO, Dolder H*

Insectes Sociaux

*E-mail: heidi@unicamp.br

A040-04 Abnormal production of Transforming Growth Factor and Interferon gamma by peripheral blood cell of patients with multidrug-resistant Pulmory Tuberculosis in Brazil

Diasbardales BM, Castro AZ, Oliveira EC, Garcia RC, Afiune JB, Paschoal IA, Santos L MB*

The aim of the present study was to determine the immune response profile that differentiates patients with newly diagnosed (non treated) pulmonary tuberculosis from multidrug-resistant ones, as well as from healthy, tuberculin positive individuals. Patients with active disease showed a diminished proliferative response to PPD while multidrugs resistante patient showed a proliferative response to PPD compared to health tuberculin positive individuals. Either newly diagnosed as multidrugs resistant patients showed a diminished proliferative response of lymphocytes to PHA a lower production of IFN and increased amounts of Transforming Growth Factor beta

The Journal of Infection

*E-mail: leonilda@unicamp.br

A041-04 Detection of Brazilian bovine respiratory syncytial virus (BRSV) strain by a RT-nested-PCR in experimentally infected calves.

Almeida RS, Spilki FR, Roehe PM, Arns CW*

A reverse transcriptase (RT)-nested-polymerase chain reaction was standardised to detect bovine respiratory syncytial virus (BRSV), using a Brazilian isolate, in three experimentally infected calves. This followed initial tests in infected chicken embryo related cells. One animal had lesions, characterized by interstitial multifocal pneumonia, severe interstitial and subpleural emphysema, and lung consolidated areas. Lung and tracheal tissues collected 6 days after infection were analysed by RT-nested-PCR. Primers, specific for the BRSV G and F glycoproteins genes, yielded amplification fragments of 371 and 481bp, respectively, from the RNA of the cell-propagated virus. Using RNA extracted from organs of infected calves, RT-nested-PCR amplified the fragment of the G gene in all tracheal samples, but in only two of three lung samples analysed. These results suggest that RT-nested-PCR could be a promising assay for diagnosis and epidemiological analysis of BRSV in Brazil.

Veterinary Microbiology

*E.mail: arns@unicamp.br

A042-04 Seqüência da capa e da proteína P1 de isolado brasileiro do Potato vírus Y NTN

Sawazaki HE, Souza Dias JAC, Módolo DG, Caporrino MC, Stach-Machado DR*
Fitopatologia Brasileira

*E.mail: dmachado@unicamp.br

A043-04 A new species of the genus Neosilba (Diptera: Lonchaeidae)

Strikis PC, Prado AP*

Zootaxa 828

*E-mail: aprado@unicamp.br

A044-04 The effects of plant structure on the spatial and microspatial distribution of a bromeliad-living jumping spider (Salticidae)

Romero GQ, Vasconcellos-Neto J*

Journal of Animal Ecology

*E-mail: jvascont@unicamp.br

A045-04 The effects of the wood-boring Oncideres humeralis (Coleoptera, Cerambycidae) on the number and size structure of its host-plants in southeast Brazil

Romero GQ, Vasconcellos-Neto J*, Neto HFP

Journal of Tropical Ecology

*E-mail: jvascont@unicamp.br

A046-04 Spatial distribution and mcrohabitat preference of Psecas chapoda (Peckham & Peckham) (Araneae, Salticidae)

Romero GQ, Vasconcellos-Neto J*

Journal of Arachnology

*E-mail: jvascont@unicamp.br

A047-04 Characterization of the insulinotropic action of a phospholipase A2 isolated from Crotalus durissus collilineatus rattlesnake venom on rat pancreatic islets

Nogueira TCA, Ferreira F, Toyama MH, Stoppiglia LF, Marangoni S, Boschero AC*, Carneiro EM

Toxicon 45

*E-mail: boschero@unicamp.br

A048-04 A new C-type animal lectin isolated from Bothroups pirajai is responsible for the snake venom major effects in the isolated kidney

Havt A, Toyama MH, Do Nascimento NR, Toyama DO, Nobre AC, Martins AM, Barbosa PS, Novello JC, Boschero AC*, Carneiro EM, Fonteles MC, Monteiro HS

International Journal of Biochemistry and Cell Biology

*E-mail: boschero@unicamp.br

A049-04 Effects of diabetes and CETP expression on diet-induced atherosclerosis in LDL receptor-deficient mice

Berti JA, Salerno AG, Bighetti EJB, Casquero AC, Boschero AC*, Oliveira HCF

APMIS

*E-mail: boschero@unicamp.br

A050-04 Short-term in vivo inhibition of IRS-1 expression leads to insulin resistance, hyperinsulinemia and increased adiposity

Araújo EP, Souza CT, Gasparetti AL, Ueno M, Boschero AC*, Saad MJA, Velloso LA

Endocrinology 146

*E-mail: boschero@unicamp.br

A051-04 Western diet modulates insulin signaling, JNK activity and IRS-1ser307 phosphorylation in a tissue-specific fashion

Prada PO, Zechin HG, Gasparetti AL, Torsoni MA, Ueno M, Hirata AE, Amaral MEC, Hoer NF, Boschero AC*, Saad MJA

Endocrinology 146

*E-mail: boschero@unicamp.br

A052-04 Co-expression and regulation of connexins 36 and 43 in cultured neonatal pancreatic islets

Leite AR, Furtado AG, Boschero AC*, Collares-Buzato CB

Canadian Journal of Physiology and Pharmacology

*E-mail: boschero@unicamp.br

A053-04 Structure-function relationship of new crotamine isoforms from the Crotalus durissus cascavella

Toyama DO, Boschero AC*, Martins AM, Fonteles CM, Monteiro SH, Toyama HM

Protein Journal

*E-mail: boschero@unicamp.br

A054-04 Biological and structural characterization of a new PLA2 from the Crotalus durissus collilineatus venom

Toyama MH, Toyama DO, Joazeiro PP, Carneiro EM, Beriam LOS, Marangoni S, Boschero AC*

Protein Journal

*E-mail: boschero@unicamp.br

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