ARTIGOS PUBLICADOS EM PERIÓDICOS
P001-04 Patterns in foraging and nesting ecology in the neotropical ant Gnamptogenys moelleri (Formicidae, Ponerinae)
Cogni R, Oliveira PS*
We provide data on the natural history and behaviour of Gnamptogenys moelleri in a forest in SE Brazil. The species hunts for live prey and scavenges the majority of the items from dead animals. Retrieving was performed by solitary workers, or by a group of 3-12 workers recruited to the food source. In the warm period more ants left the nest to forage, and foraging trips achieved greater distances compared to the cool season. The opportunism, the small foraging area, as well as the seasonal differences in foraging activity are discussed and compared with other tropical ants.
Insectes Sociaux 51: 123-130, 2004. IF = 1.535
P002-04 Recruitment behavior during foraging in the neotropical ant Gnamptogenys moelleri (Formicidae: Ponerinae): does the type of food matter
Cogni R, Oliveira PS*
We investigated recruitment in the laboratory through a series of experiments. Three types of food were used: 50% honey solution, large insect prey, and cluster of small insects. After finding liquid food or large prey, the forager returns to the nest and transmits information to nestmates about food location on the substrate. There is no transmission of information about the location of small clumped prey, although the returning scout induces nestmates to leave the nest and hunt. This study also shows that transmission of information about food location depends on the type of food found.
Journal of Insect Behavior 17:443-458, 2004. IF = 0.861
P003-04 Interaction between ants and fruits of Guapira opposita (Nyctaginaceae) in a Brazilian sandy plain rainforest: ant effects on seeds and seedlings
Passos L, Oliveira PS*
Guapira trees are primarily dispersed by birds. Mature fruits of G. opposita fall spontaneously with the pulp intact, or are dropped by birds with bits of pulp attached. In either case ground-dwelling ants rapidly remove the fruits to the nest. The ponerine ants Odontomachus chelifer and Pachycondyla striata transport single fallen fruits to nests. Seedlings of Guapira are more frequent close to Odontomachus nests than away from these. Fruit displacement by ponerines affect seeds and seedlings, and illustrate the complex nature of the dispersal ecology of tropical tree.
Oecologia 139: 376-382, 2004. IF = 2.899
P004-04 Ant-Plant-Herbivore Interactions in the Neotropical Cerrado Savanna
Oliveira PS*, Freitas AVL
We discuss recent evidence suggesting that occurrence of liquid reward on leaves plays a key role in mediating the foraging ecology of foliage-dwelling ants, and that facultative ant-plant mutualisms are important in structuring the community of canopy arthropods. Ant-mediated effects on cerrado herbivore communities can be revealed by experiments performed on wide spatial scales, including many environmental factors such as soil fertility and vegetation structure.
Naturwissenschaften 91: 557-570, 2004. IF = 2.050
P005-04 Modulation of gastrin and epidermal growth factor by pyrrolizidine alkaloids obtained from Senecio brasiliensis in acute and chronic induced gastric ulcers
Toma W, Trigo JR, Bensuaski de Paula AC, Brito ARMS*
We investigated the antiulcerogenic activity of pyrrolizidine alkaloids (PAs) integerrimine, retrorsine, senecionine, usaramine and seneciplhylline, an alkaloidal extract obtained from Senecio brasiliensis. The effects of PA extract were dose dependent. The mechanisms implicated on this activity were evaluated by determination of gastrin plasma levels in rats subjected to the acute treatment with PA extract and by expression of mRNA of Epidermal Growth Factor (EGF) after chronic treatment with this extract. The results showed that the PA extract increased both the levels of gastrin and the expression of EGF on these animals. Histological examinations showed a reduction of exfoliation of superficial cells, hemorrhages and blood cell infiltration. In conclusion, the PAs showed an important and qualitative antiulcerogenic activity mediated by increase in gastrin secretion and mRNA expression of EGF.
Canadian Journal of Physiology and Pharmacology 82: 319-325, 2004. IF = 1.603
P006-04 Preventive activity of pyrrolizidine alkaloids from Senecio brasiliensis (Asteraceae) on gastric and duodenal induced ulcer on mice and rats
Toma W, Trigo JR, Bensuaski de Paula AC, Brito ARMS*
The alkaloid extract of Senecio brasiliensis inflorescences contain a mixture of the pyrrolizidine alkaloids (PA) senecionine, integerrimine, retrorsine, usaramine and seneciphylline. The PA mixture was investigated on preventive antiulcerogenic effects on standard rodent models of induced gastric and duodenal ulcers. In the HCl/ethanol, indomethacin–bethanechol and hypothermic-restraint-induced gastric ulcer, the lesion was significantly inhibited by PA. In the pylorus-ligature, PA (i.d.), significantly increased the gastric juice content and the pH values and decreased the acid output. In the cysteamine induced duodenal ulcers, PA (p.o.) showed significant inhibition of the duodenal lesions when compared to the respective control. The results suggested that the PA extract from Senecio brasiliensis inflorescences presents a significant anti-ulcer effect in the selected ulcer models, involving cytoprotection. Additional studies on other possible mechanisms involved with effect of the PA as anti-ulcer agents are in progress.
Journal of Ethnopharmacology, 95: 345-351, 2004. IF = 1.420
P007-04 Pyrrolizidine alkaloids in three Senecio species from southern Brazil
Paiva JA, Barata LES, Trigo JR*
Aerial parts of Senecio conyzifolius Baker and inflorescences of S. oleosus Vell. (Asteraceae: Senecioninae, Section Corymbosi, Subsection Viscosi), and aerial parts of S. bonariensis Hook. & Arn. (Section Hualtatini) were sampled for pyrrolzidine alkaloids (hereafter PAs). The plants were collected in southern Brazil (São Joaquim-SC, Bom Jardim da Serra-SC, Guaíba-RS, respectively). The vouchers were deposited in the Herbarium of the Departamento de Botânica, Instituto de Biologia, UNICAMP, Campinas, SP, Brazil (UEC) and the Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil (ICN) (UEC120299, UEC120298, ICN110163, respectively). Plants of the genus Senecio have PAs; these compounds have toxic activities, and also take part in some ecological interactions mediating plant-insect-predator relationships. Recently Trigo et al. (2003) analysed PAs from 14 Senecio species from southern Brazil by GC-MS. However it is needed to isolate, and identify these compounds by 1H and 13C NMR, since the elution times of some diastereoisomers by GC analysis are very close. This paper describes the isolation and identification of known pyrrolizidine alkaloids from new sources cited above, giving more complete 1H NMR data on platyphylline.
Biochemical Systematics and Ecology 32: 1219-1222, 2004. IF = 0.704
P008-04 Semiochemicals derived from pyrrolizidine alkaloids in male ithomiine butterflies (Lepidoptera: Nymphalidae: Ithomiinae)
Schulz S, Beccaloni G, Brown Jr KS, Boppré M, Freitas AVL, Ockenfels P, Trigo JR*
Analysis of male hairpencil components of 54 species in 30 genera of Ithomiinae butterflies showed the presence of 13 volatile compounds formed by hydrolysis, oxidation, lactonization and/or methylation of both necic acid and pyrrolizidine base portions of lycopsamine, an alkaloid widespread in nature and known to be specifically sought, ingested and stored by these insects. Six of these compounds have not been reported before from insects. Comparison of occurrence of components with proposed phylogenies of the 50 or more genera in the subfamily did not give clear patterns; more specific structures, including a lactone resulting from specific oxidation of an unactivated methyl group, were more typical of apomorphic genera.
Biochemical Systematics and Ecology 32: 699-713, 2004. IF = 0.704
P009-04 Anestésicos locais: interação com membranas de eritrócitos de sangue humano, estudada por Ressonância Magnética Nuclear de 1H e 31P”
Fraceto LF, de Paula E*
Local anesthetics can affect directly the sodium channel protein or can non-specifically interact with the membrane lipids. 1H and 31P nuclear magnetic resonance spectroscopy allowed determination of interactions between LA and human erythrocyte membranes. Lidocaine and Benzocaine bind to the membranes, increasing the mobility of the lipid’s acyl chain, decreasing the mobility and/or changing the structure of the polar head-groups. While Lidocaine seems to be inserted preferentially at the polar interface of the membrane, by electrostatic and hydrophobic interaction with the lipids, Benzocaine prefers a little deeper insertion, nearby the glycerol and hydrophobic core regions. Changes in organization of the lipid molecules may be the key to stabilize the Na+-channel in its inactivated state.
Química Nova 27: 66-71, 2004. IF = 0.627
P010-04 Liposomal Systems for Bupivacaine and Mepivacaine: preparation, characterization and evaluation by sciatic nerve blockade
Araújo DR, Cereda CMS, Brunetto GB, Pinto LMA, Santana MHA, de Paula E*
Liposomal formulations are able to control drug-delivery in biological systems prolonging the anesthetic effect. We have evaluated in vivo liposome systems for bupivacaine (BVC) and mepivacaine (MVC) prepared at clinical effective concentrations. Liposomal formulations did not modify motor blockade compared to their plain solutions. In sensory blockade liposomal BVC did not show any advantage, relatively to the plain BVC while liposomal MVC improved intensity/duration of the sensory blockade. Liposomal MVC is a potentially new drug-delivery system for pain treatment, reaching a potency comparable to that of BVC.
Canadian Journal of Anesthesia-Journal Canadien D’Anesthesie 51: 566-572, 2004. IF = 1.208
P011-04 A new look at the hemolytic effect of local anesthetics, considering their real membrane/water partitioning at pH 7.4
Malheiros SVP, Gottardo L, Pinto LMA, Yokaichiya DK, Meirelles NC, de Paula E*
The contribution of structure/physicochemical properties of local anesthetics (LA) to membrane partition and solubilization were studied with hemolytic experiments. At pH 7.4, the binding of uncharged and charged species of 9 clinically used LA to the membranes was considered, regarding its effect on the ionization constant. The hemolytic effect was greatly influenced by the uncharged LA species, as if it was ruled by hydrophobic interaction. Considering the strong correlation between the hemolytic activity and LA potency, there is indication that hemolysis could be a predictive trial for the development of new LA.
Biophysical Chemistry 100: 213-221, 2004. IF = 2.102
P012-04 Inclusion compounds between beta-Cyclodextrin: local anesthetics a theoretical and experimental study using differential scanning calorimetry and molecular mechanics
Pinto LMA, de Jesus MB, de Paula E*, Lino ACS, Duarte HÁ, Takahata Y
Bupivacaine (BVC) and Lidocaine (LDC) were studied theoretically and experimentally. A drug-delivery system with cyclodextrin (CD) was prepared for both LA and characterized with Differential Scanning Calorimetry. The enthalpy changes for LA:CD association were calculated using Molecular Mechanics and OPLS Forced Fields, revealing differences between 1:1 and 1:2 stoichiometries of ca. 1 kcal.mol-1 for BVC and 10 kcal.mol-1 for LDC. Present findings reveal that ring-out type of docking is stable for the 1:1 stoichiometry once head-to-head alignment is stable in the 1:2 stoichiometries. 1:1 and 1:2 associations coexist and establish a dynamic equilibrium.
Theoretical Chemistry Accounts 678: 63-66, 2004. IF = 2.209
P013-04 Liposomal prilocaine: preparation, characterization, and in vivo evaluation
Cered CMS, Araújo DR, Brunetto GB, de Paula E*
The development and in vivo evaluation of a liposomal system for prilocaine is reported in terms of liposome size, effect of prilocaine on membrane fluidity and antinociceptive effect of liposomal vs. plain prilocaine solution (in a rat infraorbital nerve blockade model). Encapsulation enhanced the anesthetic effect in comparison to plain prilocaine. It is suggested that liposomal prilocaine facilitates the controlled release of the anesthetic, increasing the sensory nervous blockade, thus being a choice to replace vasoconstrictor-containing local anesthetic formulations in odontology.
Journal of Pharmacy and Pharmaceutical Sciences 7: 235-257, 2004. IF = 1.067
P014-04 Influence of Liposomal-Local Anesthetics on Platelet Aggregation in vitro
Pinto LMA, Pereira R, de Paula E*, de Nucci G, Santana MHA, Donato JL
The effect of three clinically used local anesthetics (LA) on the platelet aggregation induced by ADP was assessed. Liposomal formulations of the three LA were also tested. LA molecules inhibited platelet aggregation (bupivacaine>lidocaine>benzocaine). Liposomal LA showed increased inhibitory effects, showing that aggregation tests could be used to assess the toxicity of new drug formulations.
Journal of Liposome Research 14: 51-59 , 2004. IF = 1.259
P015-04 Pharmacological and histopathological characterization of Bothrops lanceolatus (Fer de lance) venom induced edema
Guimarães AQ, Cruz-Höfling MA*, Araújo PMF, Bon C, Lôbo de Araújo A
Bothropic venoms induce severe local damage caused by the presence of myotoxic, hemorrhagic and edema-inducing toxins. The paw edema produced by Bothrops lanceolatus in mice, is mainly due through the release of cyclooxygenase and lipooxygenase compounds. Local hemorrhage accompanies these effects. Here, we examined which mediators are involved, and the action of EDTA in neutralizing edematogenic and hemorrhagic activities. Dexamethasone, methysergide, HOE 140 and mepyramide reduced significantly the edema formation, but indomethacin was ineffective. The inflammatory picture, which affected connective tissue dermal paw, was partially reverted by EDTA. We conclude that the edema formation caused by B. lanceolatus venom was partially dependent on a hemorrhaging since heating the venom edema is greatly inhibited. Metabolites of the arachidonic acid, bradykinin, histamine and serotonin were also involved in the edema effect.
Inflammation Research 53: 284-291, 2004. IF = 1.450
P016-04 The effects of low laser irradiation on angiogenesis in injured rat tibiae
Garavello I, Baranauskas V, Cruz-Höfling MA*
The repair or regeneration of damaged tissues parallel with neoformation of blood vessels. In this work we examined the influence of low-level laser therapy (LLLT) in the formation of new blood vessels in a surgically injured area of rats tibiae. Daily doses of HeNe laser irradiation were transcutaneously applied at the injured area over 7 and 14 days. Two incident energy densities were tested (31.5 and 94.5 Jcm-2). LLLT significantly increased the number of blood vessels sun 7 days after daily irradiation with 94.5 Jan-2, but decreased significantly after 14 days, independent of the incident energy density used. LLLT also accelerated bone matrix deposition, an indirect indication that osteoblasts were activated. Control rats did not show significant changes in the number of blood vessels, indicating that LLLT can accelerate angiogenesis.
Histology and Histopathology 19: 43-48, 2004. IF = 1.931
P017-04 Neurotoxic and myotoxic actions of crotoxin-like and Crotalus durissus cascavella whole venom in the chick biventer cervicis preparation
Beghini DG, Rodrigues-Simioni L, Toyama MH, Novello JC, Cruz-Höfling MA*, Marangoni S
The characterization of the biological properties of snake venoms may contribute both to the clinical treatment of snakebite victims, and also provide basis for structure-function studies. Chick biventer cervicis preparations were used to investigate whether Crotalus durissus terrificus rattlesnake venom and its main crotoxin-like fraction possess myotoxic and neurotoxic actions. The venom and crotoxin (0, 2, 1 and 5 g/ml) induced a dose-dependent irreversible blockade of the neuromuscular transmission indicating that both are neurotoxic. The myotoxicity assessed by light microscopy of biventer cervicis histological sections was mainly observed at higher doses of venom and crotoxin-like fraction. We conclude that both have presynaptic-acting neurotoxins since they did not change the response of cholinoreceptor agonists.
Toxicon 43: 255-261, 2004. IF = 1.862
P018-04 Pharmacological evidence for a presynaptic action of venoms from Bothrops insularis (jararaca ilhoa) and Bothrops neuwiedi (jararaca pintada)
Rodrigues-Simioni L, Zamunér SR, Cogo JC, Borja-Oliveira CR, Prado-Franceschi JP, Cruz-Höfling MA*, Corrado AP
Bothrops snake venoms typically are known to possess postsynaptic - acting neurotoxins and myotoxins, whereas Crotalic venoms classically are known to have presynaptic – acting neurotoxins. Here, B. insularis, B. neuwiedi and Crotalus durissus terrificus venoms were compared in its potentiality to act presynaptically in chick biventer cervicis preparations. All venoms blocked irreversibly neuromuscular blockade, being C. d. terrificus the faster to produce 50% blockade, and B. neuwiedi the slowest. Crotalic anti-venom protect against neuromuscular blockade produced by bothropic venoms. The results indicate that B. neuwiedi and B. insularis venoms may contain components similar to those of C. d. terrificus, which act presynaptically at lower concentrations.
Toxicon 43: 633-638, 2004. IF = 1.862
P019-04 Comparison of the neurotoxic and myotoxic effects of Brazilian Bothrops venoms and their neutralization by commercial antivenom
Zamunér SR, Cruz-Höfling MA*, Corrado AP, Hyslop S, Rodrigues-Simioni L
Bothrops species venom from B. jararaca, B. jararacussu, B. moojeni, B. erythromelas and B. neuwiedi snakes were compared in their ability to impair neuromuscular transmission and cause myonecrosis in chick biventer cervicis preparations. Pre-incubation of the venoms with bothropic antivenom for 30 min (37C) were done to examine the extension of neutralization against the venom toxic effects and revealed that the best neutralization was B. jararaca (93%) > B. erythromelas (65,8%) > B. moojeni (30,7%) > B. neuwiedi (20%) > B. jararacussu (no neutralization). Comparative results showed that it is variable the degree of neuromuscular blockade in chick nerve-muscle preparations, myonecrosis was greatest for B. jararacussu and least for B. jararaca venom. It was concluded that components of the venoms responsible for these neuromuscular effects might differ among these venoms.
Toxicon 44: 259-271, 2004. IF = 1.862
P020-04 The presynaptic activity of Bothrops toxin-I a myotoxin from Bothrops jararacussu snake venom
Oshima-Franco Y, Leite GB, Belo CA, Hyslop S, Prado-Franceschi J, Cintra AC, Giglio JR, Cruz-Höfling MA*, Rodrigues-Simioni L
Manganese ions have long been known to act pre-synaptically at neuromuscular junctions. In this work Mn2+ was used as a tool to investigate a possible pre-synaptic action of bothrops toxin (BthTX-I), a major toxin of the Bothrops jararacussu snake venom whose post-synaptic effect is already well-know. Present results showed that pre-treating mouse phrenic nerve-diaphragm preparations with Mn2+ (0.9 and 1.8mM) prevents neurotransmission blockade caused by BthTX-I. Post-neurotransmission with Mn2+ did not revert the blockade, but washing of the preparations returned twitch-tension to pre-Mn2+ levels. The results indicate that BthTX-I besides its well-established myotoxic effects, also acts pre-synaptically.
Basic & Clinical Pharmacology & Toxicology 95: 175-182, 2004. IF = 1.3442
P021-04 Mechanisms involved in the blood-brain barrier increased permeability induced by Phoneutria nigriventer spider venom in rats
Le Sueur LP, Collares-Buzato CB, Cruz-Höfling MA*
The venom of Phoneutria nigriventer armed-spider posses neurotoxic activities and may cause convulsion in experimental rats, and in severe humans accidents. We found that the venom causes blood-brain barrier breakdown (BBBb) in rats, what may explain these symptoms. Here we investigated the mechanisms involved in the BBBb in vivo and in vitro. The BBBb through the paracellular route was assessed by examining the expression of some endothelium-associated junction proteins, and through the transcellular route by using colchicines before intravenous venom injection. Observations were made 24 h after envenoming. The findings indicated that at this time interval the increased permeabilization of the BBB occurs through an enhanced microtubule-mediated trans-endothelial vesicular transport. At this time the paracellular barrier is intact.
Brain Research 1027: 38-47, 2004. IF = 2.389
P022-04 Lonomia obliqua caterpillar venom increases permeability of the blood brain barrier in rats
Silva GH, Hyslop S, Cruz-Höfling MA*
Accidents by Lonomia obliqua (Walker) caterpillars cause a hemorrhagic syndrome generally fatal to the victims. Systemic effects include blood uncoagulabulity, renal failure and eventually intra-cerebral hemorrhage. In this work we used light and electron microscopic to analyze qualitatively and quantitatively the effects of the intra-venous (i.v.) injection of the venom (200 g/kg) or five semi-purified fractions in the blood-brain barrier of rats. Cerebellum and hippocampus of control and treated rats were examined at various time intervals after envenoming. Anesthetized rats were perfused trans-cardially with the fixative and an extracellular tracer (LaNO3). BBB breakdown was assessed by the extravasations of the tracer. Soon after 6 h of envenoming cerebellar edema was seen, and by 18 h both cerebellar and hyppocampal vessels showed tracer leakage. The number of leaky vessels decreased until 72 h after envenoming. None of the fractions induced significant number of permeabilized vessels. We conclude that L. obliqua caterpillar venom contains substances able to disrupt BBB. However, the reason why semi-purified fractions were ineffective is not clear.
Toxicon: 625-634, 2004. IF = 1.862
P023-04 Biology of a tropical intertidal population of Cerithium atratum (Born, 1778) (Mollusca, Gastropoda)
Denadai MR, Amaral ACZ*, Turra A
The spatial distribution, population structure and secondary production of the gastropod Cerithium atratum were studied in the intertidal region of Engenho d’Água Beach in São Sebastião Channel on the northern coast of the State of São Paulo, Brazil, from May 1998 to April 1999. Twenty monthly samples were taken in a 0.25 x 0.25 m quadrat in two areas (A and B). Abundance and mean shell size were significantly related to period of the year and intertidal level, although any consistent pattern could be described when comparing the two sampling areas due to significant interaction between time and area. Migratory events were evidenced by marked year round variations in the frequency of individuals in the largest size classes. Thus, the size structure of this population could not be explained by recruitment and growth alone. The intertidal population was composed of annual cohorts formed by individuals recruited in different years. Each cohort could be divided into annual cohorts, with closely spaced recruitment periods in the same breeding season. The growth and annual P/ ratio was estimated for the first year of life of the recruits from 1998 occupying the intertidal region. Individuals may have an increment of 15 mm in shell length during one year, but the growth was more pronounced in summer months. The high value of P/ (3.902) was a consequence of the dominance of small-sized individuals, which have a higher weight-specific increment than large-sized ones.
Journal of Natural History 38: 1695-1710, 2004. IF = 0.514
P024-04 Feeding behavior of Scolelepis sp. (Polychaeta: Spionidae)
Pardo EV, Amaral ACZ*
The feeding behavior of the spionid Scolelepis sp. from the intertidal region of Cabelo Gordo beach (São Sebastião, state of São Paulo) was studied in the laboratory. In still water, Scolelepis sp. captures particles on the surface of the sediment with spinning movements of the distal end of its palps. During these movements, the prostomium is kept out of the gallery. When a particle is captured, the palps coil helically and bring the particle to the partially everted pharynx for ingestion. When deposited particles are suspended by an air jet with the aid of a pipette, the animal feeds from the water column. The same behavior was observed when plankton was added to the water. Thus, environmental conditions such as hydrodynamism influence the suspension-feeding behavior of Scolelepis sp., and this species opportunistically changes its feeding behavior
Brazilian Journal of Oceanography 52: 75-79, 2004.
P025-04 Feeding behavior of the cirratulid Cirriformia filigera (Delle Chiaje, 1825) (Annelida: Polychaeta)
Pardo EV, Amaral ACZ*
Observations on the feeding behavior of Cirriformia filigera (delle Chiaje, 1825) (Annelida: Polychaeta) from the intertidal region of São Francisco and Engenho D’água beaches (São Sebastião, state of São Paulo) were made in the laboratory. This species, like other cirratulids, is a deposit feeder, feeding mainly on the surface of the sediment. When feeding, the worms extend about 4 palps out of the sediment and touch the surface, capturing particles. The collected food particles pass slightly over the groove of each palp and go straight to the mouth. Only fine sand grains were captured. Cirriformia filigera lies just beneath the surface of the substrate, in a J-shaped tube. It extends about 4 branchial filaments into the overlying water for aeration. These grains pass along the ventral region of the animal until they reach the middle part of its body. At this point, the palps and branchial filaments take over, moving the sand grains to the posterior end of the dorsal side of the animal, covering this area with them. Some sand grains were also seen to be ingested during these movements
Brazilian Journal of Biology 64: 283-288, 2004.
P026-04 Bathyglycinde profunda (Hartman & Fauchald) (Polychaeta: Goniadidae): new combination
Rizzo AE, Amaral ACZ*
Goniadid polychaetes belonging to the genus Bathyglycinde Fauchald, 1972 were collected during the REVIZEE Program/South Score/Benthos on the outer shelf and continental slope off the south southeastern coast of Brazil. This genus is recorded for the first time from Brazilian waters. A new combination for the species Glycinde profunda Hartman & Fauchald, 1971 is proposed, and the species is illustrated. This species was originally assigned to the genus Glycinde Müller; 1858, but the conspicuous proboscideal papillae and the simple capillary notochaetae without knobs are typical of Bathyglycinde. Bathyglycinde profunda comb. nov. differs from other congeneric species mainly in having all chaetigers with single pre- and postchaetal lamellae, proboscideal papillae in Area I and 32-34 uniramous chaetigers. This is the first member of the genus found in less than abyssal depths. A key to the species of Bathyglycinde is provided.
Revista Brasileira de Zoologia 21: 410-415, 2004.
P027-04 Progoniada and Goniadella (Annelida: Polychaeta: Goniadidae) from the outer continental shelf slope off the south-eastern Brazil.
Rizzo AE, Amaral ACZ*
Species of the genera Progoniada and Goniadella are recorded from off the Brazilian coast, from depths of 93 to 808 m. The description of Progoniada regularis is complemented with new data on the number of chevrons and micrognaths. Goniadella revizee n. sp. is described; it differs from the five known species of the genus mainly in having acicular chaetae above the dorsal cirrus, together with 20-21 uniramous chaetigers. The proboscideal papillae of both species are illustrated by scanning electron micrographs.
Journal of the Marine Biological Association of the United Kingdom 84: 47-58, 2004. IF= 0.781
P028-04 Aluminium triggers necrosis and apoptosis in V79 cells
Schildknecht PHPA, Vidal BC*
Toxicology and Environmental Chemistry 86: 63-72, 2004.
P029-04 Histochemical analysis of the root extracellular layer in maize and wheat
Schildknecht PHPA, Castro M, Vidal BC*
Canadian Journal of Botany 82: 1419-1428, 2004. IF = 1.194
P030-04 Increased DNA content and nuclear phenotypic alterations associated with mechanical load in wrap-around joint chicken tendons
Paoli F, Vidal BC*
Fibroblasts in tendon cartilage respond to mechanical stimuli through metaplasia and the expression of new synthetic activities following gene activation. Here we investigated changes in nuclear phenotypes of Feulgen-stained fibroblasts of 21- and 41-day-old-chicken tendons (calcaneus communis and flexor digitorum superficialis) considered to differ in response to mechanical load, as assessed by video image analysis. Compared to diploid chicken erythrocytes, the mentioned fibroblasts had larger DNA amounts representing the G2 phase and/or polyploidy. In both tendons a higher DNA amount and more packed chromatin was found in 41-day-old chickens. Data resulting from the analysis of other parameters reveal that the fibroblast nuclei in both tendon types respond to compressive load around the articulation type involved.
Brazilian Journal of morphological Sciences 21: 211-216, 2004.
P031-04 Feulgen staining in Malpighian tubules of meliponid bees: a methodological contribution
Mampumbu AR, Vidal BC, Mello MLS*
Whole-mounted Malpighian tubules of larvae from Melipona quadrifasciata and M. rufiventris fixed in acetic ethanol showed a positive cytoplasmic staining with Schiff reagent when submitted to the Feulgen reaction in which acid hydrolysis was performed in 4M HCl at 25oC (plasmal reaction), jeopardizing the evaluation of the nuclear Feulgen-DNA values. The ability of various treatments used prior to the Feulgen acid hydrolysis to abolish the plasmal reaction was examined. Phospholipid aldehyde groups present in the cytoplasm of the Malpighian tubules were found to be blocked by sequential treatment with 5% sodium borohydride and acetone-chloroform (1:1, v/v) 15 min each prior to HCl hydrolysis and avoiding the plasmal reaction.
Brazilian Journal of morphological Sciences 21: 31-33, 2004.
P032-04 Cell death evaluation in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17
Mello MLS*, Barbisan LF, Lareef MH, Russo J, Vidal BC
The incidence of apoptosis and catastrophic cell death were investigated in the benzo[a]pyrene (BP)-transformed human breast epithelial cell line BP1-E after microcell-mediated transfer of chromosomes 11 and 17. Considering that the introduction of these chromosomes to BP1-E cells reverted some of these cells’ characteristics, including those affected by microsatellite instabilities (MI) and loss of heterozygosity (LOH), to those of the parental non-transformed MCF-10F cells, it would also be expected that the cell death rates would be affected. The transfer of the mentioned chromsomes, especially chromosome 17 to BP1-E cells increased the apoptotic ratios and decreased the nuclear instability ratios, and that MI and LOH induced by BP affect the control of cell death mechanisms.
Mutation Research – Fundamental and Molecular Mechanisms of Mutagenesis 546: 39-43, 2004. IF = 3.730
P033-04 Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology
Beletti ME, Mello MLS*
Toluidine blue staining and response to the Feulgen reaction were compared in sperm cells from rabbits with unknown fertility in order to assess chromatin alteration. The relationship between sperm morphology and chromatin alteration was also investigated. Seven hundred ejaculates were examined. The results indicated that sperm chromatin structure affected sperm head morphology. The toluidine blue staining was found to be more approppriate to characterize the intensity of chromatin alteration. When the final chromatin compaction which occurs in the epididymus was affected, secondary sperm defects could also be detected.
Theriogenology 62: 398-402, 2004. IF = 1.640
P034-04 Fluoro-Jade, but not Fluoro-Jade B, stains non-degenerating cells in brain and retina of embryonic and neonatal rats
Fernandes AMAP, Morelli CVM, Campos CBL, Mello MLS, Castilho RF, Langone F*
Fluoro-Jade and Fluoro-Jade B are used to stain brain cells under degeneration. Here, we investigated the Fluoro-Jade staining of nondegenerating cells in embryonic and neonatal rat brain and retina. In contrast to Fluoro-Jade, Fluoro-Jade B did not stain nondegenerating cells in embryonic and neonatal rats. The results indicate that in addition to staining degenerating brain cells, Fluoro-Jade also stains nondegenerating central nervous system cells in embryonic and neonatal stages.
Brain Research 1029: 24-33, 2004. IF = 2.389
P035-04 Acid phosphatase activities during the germination of soybean (Glycine max) seeds
Prazeres JN., Ferreira CV, Aoyama H*
Characteristics of germination and acid phosphatase activities were determined during soybean seeds germination. Two peaks of enzyme activities were observed after germination, for p-nitrophenylphosphate and inorganic pyrophosphate. Tyrosine phosphate, glucose-6-phosphate and phosphoenolpyruvate showed to be poor substrates; no enzyme activity was detected with phytic acid. The decrease of inorganic phosphate content during germination was coincident with the appearance of phosphatase activity. The protein content increased up to the 5th day and decreased until the 8th day. Considering the acid phosphatase activity in the cotyledons as 100%, hypocotyl and roots contained 82% and 38% of activities, respectively.
Plant Physiology and Biochemistry 42: 15-20, 2004. IF = 1.414
P036-04 Effect of chaotropic agents on the reversible unfolding of a soybean (Glycine max) seed acid phosphatase
Cavagis ADM, Granjeiro PA, Ferreira CV, Aoyama H*
Denaturation of soybean seed acid phosphatase by urea and guanidinium chloride was studied based on the intensity of tryptophan fluorescence. The conformational stability, G (H2O), was determined to be 2.48 kcal mol-1. An additional parameter, center of mass, obtained from the fluorescence data, was used to assess conformational changes. Our results suggest a complete enzyme inactivation before full enzyme unfolding.
Phytochemistry 65: 831-836, 2004. IF = 2.101
P037-04 Effect of homologous series of n-alkyl sulfates and n-alkyl trimethylammonium bromides on low molecular mass phosphotyrosine protein phosphatase activity
Granjeiro JM, Miranda MA, Maia MGST, Ferreira CV, Taga EM, Aoyama H*, Volpe PLO
Effects of anionic and cationic surfactants were studied on protein tyrosine phosphatase (PTP) activities. The enzyme was completely inactivated in the presence of anionic surfactants, in a process independent of the pH, and dependent on the chain length of the surfactants. The effect of cationic surfactants on the enzyme activity was pH-dependent; only at pH 7.0 full inactivation was observed. Inorganic phosphate protected the enzyme against inactivation by the surfactants. The inactivation of the PTP by anionic and cationic surfactants involved both electrostatic and hydrophobic interactions, and the interactions probably occurred at or near the active site.
Molecular and Cellular Biochemistry 265: 133-140, 2004. IF = 1.714
P038-04 The thermal stability of a castor bean seed acid phosphatase
Granjeiro PA, Cavagis ADM, Leite LC, Ferreira CV, Granjeiro JM, Aoyama H*
The effect of temperature was studied on the activity and structural stability of castor bean seed acid phosphatase. The activation energy with p-nitrophenylphosphate (p-NPP) as substrate was 55.2 kJ mol-1. Thermal inactivation studies showed an influence of pH in the loss of enzymatic activity at 60oC. The reaction products inorganic phosphate – Pi - (10 mM) and p-nitrophenol–p-NP - (10 mM) protected the enzyme against thermal inactivation.
Molecular and Cellular Biochemistry 266: 11-15, 2004. IF = 1.714
P039-04 Adapting a Biochemistry Course to Distance Education
Yokaichiya DK, Galembeck E*, Torres BB
Biochemistry and Molecular Biology Education 32: 27-29, 2004. IF = 0.513
P040-04 AMPc - Sinalização Intracelular: Um software Educacional
Yokaichiya DK, Fraceto LF, Miranda MA, Galembeck E*, Torres BB
Química Nova 27: 489-491, 2004. IF = 0.627
P041-04 O que alunos de diferentes cursos procuram em disciplinas extracurriculares de Bioquímica?
Yokaichiya DK, Galembeck E*, Torres BB
Revista Brasileira de Ensino de Bioquímica e Biologia Molecular 01/2004, Artigo E -
P042-04 Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens
Arroteia KF, Joazeiro PP, Yamada AT, Tanaka H, Nishimune Y, Pereira LAV*
The epididymis participates in sperm maturation leading fertilization, but little is known about the specific epididymal molecules involved in the modification of sperm. In this study, we characterized the new pattern of expression of an antigen expressed in epididymal and vas deferens epithelial. The molecules synthesized and released by epididymal and vas deferens epithelia into their lumen seem to bind on spermatozoa moving down through the ducts. The antigens identified in epididymal and vas deferens epithelial cells are expressed independently of testicular germ cells and produced in an androgen-dependent manner.
Journal of Andrology 25: 914-921, 2004. IF = 2.394
P043-04 Esophageal atresia and other visceral anomalies in a modified Adriamycin rat model and their correlations with amniotic fluid volume variations
Franca WMG, Goncalves A, Moraes SG, Pereira LAV*, Sbragia L
The Adriamycin fetuses rat model has been used to explain mechanisms of malformations in foregut division. Our study using 2.2 mg/kg of ADR for 2 days only, intraperitonealy in pregnant rats, is presented. ADR was found to be adequate to produce esophageal atresia (EA) and other visceral malformations. The use of ADR in a higher dosage for a shorter period of time produced better results than those reported in the literature. The increase of amniotic fluid volume obtained in fetuses presenting EA plus bilateral Uretero-hydronephrosis strongly suggests that a malformed communication may exist between the urinary system and the amniotic cavity.
Pediatric Surgery International 20: 602-608, 2004. IF = 0.493
P044-04 Revisão e Proposta de Nomenclatura para Biomateriais
Carvalho PSP, Bassi APF, Pereira LAV*
Biomaterial é qualquer substância ou combinação de substâncias de origem natural ou sintética que pode ser usada por um período como parte de um sistema tratado ou como reposição de algum tecido, órgão ou função do organismo. Esse artigo tem por objetivo propor uma nomenclatura para esses biomateriais, bem como definir em quais situações clínicas podem ser utilizados.
Implant News 1: 255-260, 2004.
P045-04 The usefulness of autopsies as a tool for teaching humam embryology
Moraes SG, Reis MVA, Mello MFS, Pereira LAV*
Embryology is often difficult to teach because of the rapid, three-dimensional changes that occur simultaneously on a microscopic scale. In this study, we used autopsies to develop a method to enhance the comprehension of human embryology and its associated malformations. In the first part of the class, the development of the body’s structures was explained, while in the second, images of selected autopsies were shown to the students. At the end of the course, the teaching material and the method were evaluated using a questionnaire, interviews, and a test of the knowledge. Most of the students approved the method.
Brazilian Journal of Morphological Sciences 21: 117-123, 2004
P046-04 Effects of aggrecan on Schwann cell migration in vitro and nerve regeneration in vivo
Pierucci A, Faria AM, Pimentel ER, Santos Júnior AR, Oliveira ALR*
Although the role of many small proteoglycans in regeneration of the nervous system has been established, little is known about the involvement of large proteoglycans. In this study, we evaluated the effects of aggrecan on Schwann cells in vitro and investigated its effects on axonal regeneration after sciatic nerve tubulization. The number of regenerated axons and their morphometrical parameters were determined in vivo. Aggrecan increased the number and viability of Schwann cells in vitro. Similarly, the number of regenerated fibers increased significantly when aggrecan was applied in vivo, but there were no alterations in the morphometrical parameters. These results indicate that aggrecan contributes to the regeneration of peripheral axons and has a positive effect on the Schwann cells.
Brazilian Journal of Morphological Sciences 21: 65-71, 2004.
P047-04 A role for MHC class I in synaptic plasticity and regeneration of neurons after axotomy
Oliveira ALR*, Thams S, Lidman O, Piehl F, Hökfelt T, Kärre K, Lindå H, Cullheim S
Major histocompatibility (MHC) class I molecules have recently been shown to participate in the elimination of synapses during normal development of the nervous system. Here we show an opposite role for MHC class I during synapse elimination after nerve lesion in the mature nervous system. Consequently, MHC class I is considered necessary for the selective maintenance of an inhibitory synaptic input on lesioned neurons, and its presence improves the regeneration of such neurons. This is the first demonstration that a major
immunological recognition molecule has an influence on the fate of a neuron and its ability to repair after lesion.
Proceedings of the National Academy of Sciences of the Unided States of America, 101: 17843-17848, 2004. IF = 10.452
P048-04 Peripheral nerve regeneration through nerve tubulization
Oliveira ALR*, Pierucci A, Pereira KB
A surgical approach involving repair by nerve tubulization has been extensively used to study substances that may improve the regenerative process. An interesting feature of the tubulization technique is the possibility of filling the tube with substances that can enhance regeneration. These substances include collagen, laminin, hyaluronic acid, fibronectin and, more recently, glycosaminoglycans alone or with collagen. Biopolymers, purified glial cells, and neurotrophic factors have also been tested. By using the tubulization technique, it was possible to increase the number of regenerating fibers and the gap between the stumps.
Brazilian Journal of Morphological Sciences 21: 225-231, 2004.
P049-04 Subperiosteal hydroxyapatite implants in rats submitted to ethanol ingestion
Camilli JÁ*, Cunha MR, Bertran CA, Kawachib EY
Hydroxyapatite blocks were implanted under the periosteum of the femur and skull of 40 rats divided into two groups of 20 animals, one of them received 25% ethanol diluted in water and the other did not. Bone formation close to the hydroxyapatite implant was observed in the femur of all animals 2 weeks after the surgery, however the bone volume was lower in ethanol-treated animals. It was observed in the skulls of the ethanol-treated animals a delay in new bone formation process, as a lower bone volume, too. After 4 weeks of the implantation, just one ethanol-treated animal showed no new bone formation in the femur, while no bone formation was observed in the skulls of two other rats. On the 8th and 16th weeks, bone formation was observed in both femur and skull from both groups, although always with less volume in ethanol-treated rats. We concluded that ethanol consumption does not impair osteointegration of ceramic implants, but reduces the osteogenic capacity of periosteal cells in the femur and parietal bone of the rats.
Archives of Oral Biology 49: 747-753, 2004. F = 1.158
P050-04 Structural biology of the dystrophin-deficient muscle fiber
In this review, we discuss the components and the cellular signaling molecules associated with the dystrophin-glycoprotein complex. We then focus on the molecular organization of the neuromuscular junction and its structural organization in the dystrophin-deficient muscle fibers of mdx mice, a well-established experimental model of Duchenne muscular dystrophy.
Brazilian Journal of morphological Sciences 21: 145-152, 2004.
P051-04 Distribution of calcitonin gene-related peptide at the neuromuscular junction of mdx mice
Marques MJ, Minatel E, Guimarães AO, Santo Neto H*
In this study we used rhodamine-a-bungarotoxin and anti-CGRP IgG FITC to study AChR and CGRP distribution at the neuromuscular junction of mdx mice. Using laser scanning fluorescence confocal microscopy, it was possible to see that CGRP-like immunoreactivity had a presynaptic distribution, covering the AChRs. Thirty-four percent of dystrophic junctions were found to be labeled with CGRP compared to 80% of control endplates. It is suggested that CGRP is probably not directly involved in the altered pattern of AChR seen in dystrophin-deficient muscle fibers of mdx mice.
Anatomical Record Part A – Discoveries in Molecular, Cellular, and Evolutionary Biology 279A: 798-803, 2004. IF = 1.154
P052-04 Number and size of motor units in thenar muscles
Santo Neto H*, Meciano Filho J, Passini R, Marques MJ*
The number and size of motor units (MUs) in the thenar muscles of ten fresh adult cadavers (33-74 years old) were estimated by histological methods. The average number of MUs was 161 26 and the MU size was 93 11.5. Adductor pollicis showed a significantly greater MU size.
Clinical Anatomy 17: 308-311, 2004. IF = 0.664
P053-04 Primary nerve repair by muscle autografts prepared with local anesthetic
Santo Neto H*, Pertille A, Teodori RM, Somazz MC, Marques MJ*
We evaluated the usefulness of muscle autografts obtained immediately after graft preparation with lidocaine injections for primary nerve repair. Axon and fiber diameters, as well as myelin thickness, decreased to similar extents for grafts of both time intervals. The G-ratios in the distal stumps of both groups were not different from controls, indicating that regenerated axons had a proper level of myelination. The ultrastructural appearance of the neuromuscular junctions was similar to that of normal.
Microsurgery 24: 188-193, 2004. IF = 0.812
P054-04 Insights into the loss of muscle mass following B. jararacussu venom in mice
Santo Neto H*, Vomero VU, Marques MJ*
We investigated whether nerve lesions or impaired muscle regeneration contributed to the permanent loss of muscle mass, a long-term sequela of envenoming. The nerve fibers, Schwann cells and neuromuscular junctions had regenerated in venom-treated muscle. The total number of muscle fibers was significantly lower than in the control (617±48 versus 1235±97, respectively; n=10). These results show that the loss of muscle mass was most likely related to a decrease in the ability of the muscle to regenerate rather than to nerve lesions.
Toxicon 44: 847-850, 2004. IF = 1.862
P055-04 Influence of hormonal replacement on the ventral lobe of rat’s prostate (Rattus norvegicus albinus) submitted to chronic ethanol treatment
Satollo S, Cagnon VAH*, Carvalho CAF
The aim of this study was to analyse the tissue changes occurred in the ventral lobe of rat`s prostate with hormonal replacement associated to chronic alcohol ingestion, besides setting up a correlations of the glandular morphology with the direct and indirect action of the ethanol in the prostatic gland. Rats were divided into three groups: control – received water; alcoholic – received ethanol diluted at 20% and hormone-treated alcoholic –received ethanol diluted to 20% associated with the administering of cypionate of testosterone during the last 30 days of treatment. After 150 days of treatment, the results demonstrated reduction of the total cellular volume and disorganization of the organelles involved in the secretory process. A partial recovery of the cellular volume after treatment with testosterone was found. We concluded that the ethanol impaired the cellular morphology and that the hormonal replacement by itself did not bring about efficient remodeling of the organelles responsible for the secretory process.
Tissue & Cell 36: 417-430, 2004. IF = 0.937
P056-04 Leaf life span and nitrogen content in semideciduous forest tree species (Croton priscus and Hymenaea courbaril)
Haddad CRB*, Lemos DP, Mazzafera P
Scientia Agricola 6: 462-465, 2004.
P057-04 Gastric antiulcer activity of Syngonanthus arthrotrichus SILVEIRA
Batista LM, de Almeida AB, de Pietro Magri L, Toma W, Calvo TR, Vilegas W, Souza Brito AR.*
Biological & Pharmaceutical Bulletin 27: 328-332, 2004. IF = 1.392
P058-04 Chronic treatment with bark infusion from Croton cajucara lowers plasma triglyceride levels in genetic hyperlipidemic mice
Bighetti EJ, Souza-Brito AR*, de Faria EC, Oliveira HC
Canadian Journal of Physiology and Pharmacology 82: 387-392, 2004. IF = 1.603
P059-04 Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)
Sannomiya M, Rodrigues CM, Coelho RG, dos Santos LC, Hiruma-Lima CA, Souza Brito AR*, Vilegas W
Journal of Chromatography A 1035: 47-51, 2004. IF = 3.359
P060-04 Antiulcerogenic activity of ethanol extract of Solanum variabile (false "jurubeba")
Antonio JM, Gracioso JS, Toma W, Lopez LC, Oliveira F, Brito AR*
Journal of Ethnopharmacology 93: 83-88, 2004. IF = 1.420
P061-04 Antiulcer activity and subacute toxicity of trans-dehydrocrotonin from Croton cajucara
Rodriguez JA, Hiruma-Lima CA, Souza Brito AR*
Human & Experimental Toxicology 23: 455-461, 2004. IF = 1.176
P062-04 The [Ru(Hedta) NO](0.1-) system: structure, chemical reactivity and biological assays
Zanichelli PG, Miotto AM, Estrela HF, Soares FR, Grassi-Kassisse DM, Spadari-Bratfisch RC, Castellano EE, Roncaroli F, Parise AR, Olabe JA, de Brito AR*, Franco DW
Journal of Inorganic Biochemistry 98: 1921-1932, 2004. IF = 2.225
P063-04 Analysis of a conserved duplicated tRNA gene in the mitochondrial genome of blowflies
Lessinge, AC, Junqueira ACM, Conte FF, Azeredo-Espin AML*
A região-controle e os genes flanqueadores desta região do genoma mitocondrial foram caracterizados em 3 espécies de moscas varejeiras da família Calliphoridae: Chrysomya albiceps, Chrysomya megacephala e Chrysomya chloropyga. O aspecto mais incomum relacionado a estas estruturas foi a duplicação completa e parcial das seqüências gênicas dos tRNAIle e tRNAGln, respectivamente. O tRNAGln parcialmente duplicado é considerado um pseudogene devido à ausência de maior parte da seqüência de um gene de tRNAGln típico do DNAmt de insetos. Ao contrário, as seqüências duplicadas referentes ao gene tRNAIle permanecem conservadas e acredita-se que ambas as cópias possam ser funcionais. Estes resultados demonstram a plasticidade do genoma mitocondrial no gênero Chrysomya, especialmente em relação aos genes de tRNA adjacentes à região-controle de replicação.
Gene 339: 1-6, 2004. IF = 2.705
P064-04 The mitochondrial genome of the blowfly Chrysomya chloropyga (Diptera: Calliphoridae)
Junqueira ACM, Lessinger AC, Torres TT, da Silva FR, Vettore AL, Arruda P, Azeredo-Espin AML*
The mtDNA of the blowfly Chrysomya chloropyga was completely sequenced, presenting 15837bp. The composition was biased towards As and Ts, which accounted for 76.7% of the whole genome. The cox1 gene had a serine as the start codon, while /cox2/, /nd4/ and /nd5/ had incomplete termination codons. C. chloropyga is the first species of Diptera with 23 tRNA genes instead of the usual 22, including a complete duplication of /tRNA^Ile / and a partial duplication of /tRNA^Gln / genes. The complete sequence of C. chloropyga mtDNA will be a useful source of information for molecular and evolutionary studies in Diptera.
Gene 339: 7-15, 2004. IF = 2.705
P065-04 Isolation and Characterization of microsatelite markers in the New World Screwworm Cochliomyia hominivorax (Diptera: Calliphoridae)
Torres TT, Brondani RPV, Garcia JE, Azeredo-Espin AML
Ten polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA library from the New World screw worm (NWS), Cochliomyia hominivorax. This species is one of the most important insect pests in Neotropics, causing myiasis and, consequently, significant losses to livestock industries in these regions. Amplification of the reported loci in 30 screwworms revealed an average of 6.9 alleles per locus with expected heterozygosities ranging from 0.3831 to 0.8022. These loci should therefore enable the investigation into the genetic variability and population structure of NWS.
Molecular Ecology Notes 4: 182-184., 2004. IF = 1.175
P066-04 Bioenergy in Brazil: production, application and new developments
Santos RV, Pessoa Júnior A, Roberto IC, Menossi M*, Ortega Filho S, Penna TCV
Brazil has a well developed technology to produce energy from biomass. In 2003/2004, more than 350,000 tons of sugarcane were harvested, producing 24,200 tons of sugar and 14.4 billions of liters of ethanol, and a total of 4.3 millions of cars used only ethanol as fuel. It is estimated that the use of ethanol allowed savings of US$ 27 billions in oil importation. Several technologies are under development to use bagasse a natural fiber, sweetener, and many other fermentation-based products. Genome-based projects are helping to understand the genetics of sugarcane aiming the increase in sugarcane productivity.
Brazilian Journal of Pharmaceutical Science 40: 131-133, 2004.
P067-04 Expression, purification and characterization of a novel bZIP protein from sugarcane
Schlögl PS, Kobargb J, Moreau VH, Leite A, Sabino AA, Eberlin MN, Menossi M*
Basic leucine zipper (bZIP) proteins are transcriptional factors that regulate a large range of physiological processes, from plant development to responses to biotic and abiotic stimuli. Here we described a novel bZIP of sugarcane: SCbZIP1. Northern blot analysis showed gene expression the early stages of plant development. Electrophoretic mobility assays showed that SCbZIP1 bound to G-box, C-box and Hex DNA motifs. Phosphorylation by CKII decreased SCbZIP1 DNA-binding affinity. SCbZIP1 also formed homo- and heterodimers with truncated forms of the bZIP transcription factor Opaque 2 from Coix.
Plant Science 167: 583-595, 2004. IF = 1.389
P068-04 Identification of genes preferentially expressed in the pathogenic yeast phase of Paracoccidioides brasiliensis using suppression subtraction hybridization and macroarray differential analysis
Marques ER, Ferreira MES, Drummond RD, Felix JM, Menossi M*, Savoldi M, Travassos LR, Puccia R, Batista WL, Carvalho KC, Goldman MHS, Goldman GH
Paracoccidioides brasiliensis is the causative agent of paracoccidioidomycosis, the most prevalent systemic mycosis in Latin America. Our objective was to identify P. brasiliensis genes necessary during the pathogenic phase. We used Suppression Subtraction Hybridization and macroarray analyses. Most of the genes identiﬁed were involved in basic metabolism, signal transduction, growth and morphogenesis, and sulfur metabolism. Twenty genes, including AGS1(alpha-1,3-glucan synthase) and TSA1 (thiol-speciﬁc antioxidant), were highly expressed in the yeast cells compared to the hyphae.
Molecular Genetics and Genomics 271: 667-677, 2004. IF = 2.371
P069-04 A transcript finishing initiative for closing gaps in the human transcriptome
Sogayar MC, …. Menossi M*, … + 94 autores
A transcript finishing initiative was undertaken to identify and characterize novel human transcripts. A total of 489 transcript finishing units (TFUs) were selected for validation. An overall efficiency of 43.1% was observed. A total of 59,975 bp of transcribed sequences were obtained. They were organized into 432 exons, allowing the the definition of 211 human transcripts. A full-length cDNA sequence was not available in public databases for 21% of the validated TFUs. Computer programs failed to predict the structure of 69.2% of these TFUs. Our strategy provided a significant contribution to the definition of the complete catalog of human genes.
Genome Research 14: 1413-1423, 2004. IF = 10.382
P070-04 The genome sequence of the Gram-Positive sugarcane pathogen Leifsonia xyli subs.. xyli.
Monteiro-Vitorello CB,. ...., Pereira GAG*
The genome sequence of Leifsonia xyli subsp. xyli, which causes ratoon stunting disease and affects sugarcane worldwide, was determined. The single circular chromosome of Leifsonia xyli subsp. xyli CTCB07 was 2.6 Mb in length with a GC content of 68% and 2,044 predicted open reading frames. The analysis also revealed 307 predicted pseudogenes, which is more than any bacterial plant pathogen sequenced to date. Many of these pseudogenes, if functional, would likely be involved in the degradation of plant heteropolysaccharides, uptake of free sugars, and synthesis of amino acids. Some of the predicted pathogenicity genes appear to have been acquired by lateral transfer and include genes for cellulase, pectinase, wilt-inducing protein, lysozyme, and desaturase. The presence of the latter may contribute to stunting, since it is likely involved in the synthesis of abscisic acid, a hormone that arrests growth. Our findings are consistent with the nutritionally fastidious behavior exhibited by L. xyli subsp. xyli and suggest an ongoing adaptation to the restricted ecological niche it inhabits.
Molecular Plant-Microbe Interactions 17: 827-836, 2004. IF = 4.054
P071-04 Caste-specific gene expression in the stingless bee Melipona quadrifasciata - Are there common patterns in highly eusocial bees?
Judice CC, Carazzole MF, Festa F, Sogayar MC, Hartfelder K, Pereira GAG*
O polifenismo de castas é um fenômeno multifacetado, sendo mais evidente na capacidade reprodutiva e na longevidade diferenciada entre rainhas e operárias. Nós escolhemos a abelha sem ferrão Melipona quadrifascita para investigar as diferenças na expressão gênica entre rainhas e operárias recém nascidas. A metodologia empregada foi a do differential display-PCR e os possíveis genes diferencialmente expressos, para os quais anotação funcional estava disponível, foram validados por RT-PCR e hibridação. A expressão diferenciada foi observada para os genes codantes para miosina, kettin, citocromo P450, Rab11 e Sas 10. Exceto para kettin, todos esses genes se mostraram super expressos na casta operária.
Insectes Sociaux 51: 352-358, 2004. IF = 1.535
P072-04 Evaluation of monocot and eudicot divergence using the sugarcane transcriptome
Vincentz M*, + 32 authors + Arruda P, Menck CF
Aproximadamente 40.000 cDNAs de cana de açúcar foram comparados com seqüências de outras angiospermas. Essa análise permitiu identificar um conjunto de proteínas ou domínios protéicos comum a todas angiospermas. Outro grupo de seqüências restrito a monocotiledôneaes corresponde principalmente a seqüências evoluindo rapidamente. Os dados apresentados revelam novos aspectos das mudanças que levaram a divergência entre monocotiledôneas e eudicotiledôneas. A análise comparativa também identificou vários novos genes, assim como perdas seletivas de genes em Arabidopsis.
Plant Physiology 134: 951-959, 2004. IF = 5.881
P073-04 Phylogeny of the Nymphalidae (Lepidoptera)
Freitas AVL*, Brown Jr KS
A generic-level phylogeny for Nymphalidae was produced by cladistic analysis of 234 characters from all life stages. The 95 species in the matrix represent all important recognized lineages. Libytheinae are basal, Danainae-Ithomiinae are next, and the remaining lineages are grouped into two branches: the Heliconiinae-Nymphalinae, primarily flower-visitors, and the Limenitidinae, Biblidinae, and the satyroids, primarily fruit-attracted. Data partitions showed that the two data sets (immatures and adults) are very different; Partitioned Bremer Support showed that adult characters are the main source of conflict in the combined tree. This phylogeny supports the monophyly and relationships of most presently recognized subgroups.
Systematic Biology 53: 363-383, 2004. IF = 10.257
P074-04 A new species of Yphthimoides (Nymphalidae, Satyrinae) from southeastern Brazil
A new species of Yphthimoides (Nymphalidae, Satyrinae) is described from the highlands of the Serra do Cipó, Minas Gerais. The position of this species is still uncertain within the genus, but based on the male genitalia it is near to Y. celmis (Godart). Journal of the Lepidopterists’ Society 58: 7-12, 2004.
P075-04 Immature stages of Amphidecta reynoldsi (Nymphalidae: Satyrinae)
Amphidecta reynoldsi é uma espécie de Satyrinae com afinidades incertas dentro da subfamília. As larvas são verdes, alongadas, e com um par de escolos longos na cabeça. As pupas são verdes, alongadas e com um prolongamento fino na região cefálica. Os imaturos são bastante similares a alguns Pronophilini, mas estudos adicionais são ainda necessários antes de se definir a posição deste gênero dentro dos Satyrinae.
Journal of the Lepidopterists’ Society 58: 53-55, 2004.
P076-04 Guia das borboletas frugívoras da Reserva Estadual do Morro Grande e região de Caucaia do Alto, Cotia (SP)
Uehara-Prado MAV, Freitas AVL*, Francini RB, Brown Jr KS
As espécies de borboletas frugívoras (Lepidoptera: Nymphalidae) conhecidas da Reserva Estadual do Morro Grande (Cotia, SP) e Caucaia do Alto são reunidas para fornecer um guia de campo, ilustrado em cores, para fins de avaliação e monitoramento de áreas de interesse conservacionista.
Biota Neotropica 4: 1-25, 2004
P077-04 Two new species of Actinote (Lepidoptera, Nymphalidae) from Southeastern Brazil
Francini RB, Freitas AVL*, Penz CM
This paper describes two new species of Actinote (Nymphalidae, Heliconiinae, Acraeini) from southeastern Brazil. Actinote eberti sp. nov. occurs in the Serra da Mantiqueira region and resembles those in the Actinote black-yellow mimicry group. Actinote pratensis sp. nov. is found in widely scattered points in the rich-soil central São Paulo transition between montane and semideciduous atlantic forest, and belongs to the orange-yellow mimicry group.
Zootaxa 719: 1-10, 2004.
P078-04 Chromosome evolution in Neotropical Danainae and Ithomiinae
Brown Jr KS*, Van Schoultz B, Suomalainen E
Chromosome numbers are given for 226 species of Neotropical Ithomiinae (Nymphalidae). Twelve Neotropical Danainae (n=22, 29-31) and the Australian Tellervo (n=32) were sister groups. The numbers range from n=5 to n=120 with modal values at n=12-18, and only 16/26 counts at the usual mode of butterflies, n=30/31. Superimposition on a phylogeny suggests early halving of the complement to n=14-15, followed by variation in each genus and tribe. The evolutionary opportunism of the Ithomiinae accompanies their population biology and community ecology.
Hereditas 141: 1-21, 2004. IF = 0.690
P079-04 Trypanosoma cruzi response to the oxidative stress generated by hydrogen peroxide
Finzi JK, Chiavegatto CWM, Corat KF, Lopez JA, Cabrera OG, Mielniczki-Pereira AA, Colli W, Alves MJM, Gadelha FR*
As an intracellular parasite, Trypanosoma cruzi is exposed to reactive oxygen species. The study of the parasite antioxidant defense mechanisms may lead to the development of a more specific chemotherapy for Chagas’disease. In this work, the involvement of tryparedoxin peroxidase (TcCPX) in T. cruzi resistance to oxidant-mediated injury was investigated using wild-type and TcCPX overexpressing cells. The results suggests TcCPX involvement in T.cruzi response to oxidative stress, and that protein translation occurs accordingly to parasite needs.
Molecular and Biochemical Parasitology 133: 37–43, 2004. IF = 2.803
P080-04 Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffıi
Krauchenco S, Nagema RAP, Silva JA, Marangoni S*, Polikarpov I
The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffıi is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% at 1.8 Å resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. Structural features granting CTI high inhibitory activity are discussed.
Biochimie 86: 167–172, 2004. IF = 3.814
P081-04 A new method to purify hepatic CYP1A of Prochilodus scrofa, a Brazilian freshwater fish
Da Silva MEF, Silva JA, Marangoni S*, Novello JC, Meirelles NC
Cytochromes P450 constitute a superfamily of the phase I enzymes whose primary task is the detoxification of both endogenous and xenobiotic compounds. The aim of the present study was the purification of the hepatic isoform of CYP1A in Prochilodus scrofa, a Brazilian fish, using only one chromatographic step. The purification of CYP1A was done by Reverse Phase HPLC on a C18 column. CYP1A fractions produced a single uniform band on SDS-PAGE with an apparent molecular mass of 58 kDa. Purified CYP1A of P. scrofa showed strong cross-reactivity with antibodies directed against CYP1A from trout. This new procedure is considered efficient for purifying hepatic CYP1A from P. scrofa, and the CYP1A isoform of this fish is considered to have a highly conserved protein region. Elsevier Inc.
Comparative Biochemistry and Physiology C-Toxicology & Pharmacology 138: 67–74, 2004. IF = 1.651
P082-04 Trypsin inhibitor from Poecilanthe parviflora seeds: purification, characterization, and activity against pest proteases
Garcia VA, Freire MG, Novello JC*, Marangoni S, Macedo ML
Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves of being attacked by insects. In this work, a novel trypsin inhibitor (PPTI) was purified from the seeds of the native Brazilian tree Poecilanthe parviflora (Benth) (Papilioinodeae, Leguminosae) by gel filtration chromatography on a Sephadex G-100 followed by Superdex G75 chromatography (FPLC), Sepharose 4B-Trypsin column, and fractionated by reversed-phase HPLC on a C-18 column. SDS-PAGE showed that PPTI consisted of a single polypeptide chain with molecular mass of about 16 kDa.
Protein Journal 23: 343-50, 2004. IF = 1.099
P083-04 Anti-sera raised in rabbits against crotoxin and phospholipase A2 from Crotalus durissus cascavella venom neutralize the neurotoxicity of the venom and crotoxin
Beghini DG, Hernandez-Oliveira S, Rodrigues-Simioni L, Novello JC, Hyslop S, Marangoni S*
In this work, we examined the ability of rabbit anti-sera against crotoxin and its PLA2 subunit to neutralize the neurotoxicity of venom and crotoxin from C. d. cascavella in mouse phrenic nerve–diaphragm and chick biventer cervicis preparations.. This was confirmed by ELISA, with both anti-sera having end-point dilutions of 3 £ 1026. Anti-crotoxin serum neutralized the neuromuscular blockade in phrenic nerve–diaphragm muscle preparations at venom or crotoxin:anti-serum ratios of 1:2 and 1:3, respectively. Anti-PLA2 serum also neutralized this neuromuscular activity at a venom or crotoxin:anti-serum ratio of 1:1. These results show that anti-sera against crotoxin and PLA2 from C. d. cascavella venom neutralized the neuromuscular blockade induced by venom and crotoxin in both nerve–muscle preparations, with the anti-serum against crotoxin being slightly less potent than that against crotoxin.
Toxicon 44: 141–148, 2004. IF = 1.862
P084-04 Dichotomic spemiogenesis in Euptoieta hegesia (Lepidoptera: Nymphalidae
Mancini K, Dolder H*
Borboletas e mariposas produzem espermatozóides anucleados (apirene) e nucleados (eupirene). Neste trabalho usamos microscopia de luz e eletrônica de transmissão para investigar a espermiogênese de machos de Euptoieta hegesia. Apenas adultos foram usados por ser esta uma espécie de longa vida adulta em que todas as fases de desenvolvimento podem ser observadas. Os machos têm um único testículo fundido com cistos que contém espermatozóides apirenes ou eupirenes. Os principais eventos da espermiogênese apirene incluem a formação, transformação e eliminação de micronúcleos, formação do capuz denso, desenvolvimento dos derivados mitochondriais e alongamento da cauda.A espermatogênese eupirene envolve a formação do acrosomo, condensação nuclear, desenvolvimento dos apêndices extracelular, formação dos derivados mitochondriais e alongamento da cauda. O padrão e eventos da espermiogênese eupirene e apirene em E.hegesia corroboram e complementam os dados na literatura, principalmente em relação aos estágios intermediários de desenvolvimento de algumas estruturas, como o acrosomo, axonema e apêndices extracelulares.
Brazilian Journal of Morphological Sciences 21: 13-23, 2004.
P085-04 Structure and ultrastructure of spermatozoa of Meliponini (stingless bees) (Hymenoptera, Apidae)
Zama U, Lino-Neto J, Dolder H*
Apesar de sua importância para a reprodução de phanerogamas, poucas abelhas receberam uma descrição ultraestrutural adequada de seus espermatozóides. Neste trabalho, concluímos que é possível definir um padrão característico para espermatozóides de Meliponini. Porém também encontramos diferenças espécie-específicas que poderão ser usadas em estudos taxonômicos e filogenéticos. Espermatozóides de Meliponini são divididos em cabeça e cauda. Na cabeça encontra-se um acrosomo, com o perforatorium coberto pela vesícula acrosômica, e um núcleo. Em cortes transversais, o acrosomo é circular na extremidade e torna-se triangular a medida que se aproxima do núcleo. A base do perfuratorium penetra numa pequena cavidade no topo do núcleo. O flagelo consiste de um axonema, um par de derivados mitocondriais, o adjunto do centríolo e um par de corpos acessórios. O axonema tem o padrão típico de 9 + 9 + 2 microtúbulos. Em corte transversal, os derivados mitocondriais são elipsóides e o maior tem uma região paracristalina. O adjunto do centríolo começa na base do núcleo e estende paralelo ao axonema, até encontrar o topo do derivado menor.
Tissue & Cell 36: 29-41, 2004. IF = 0.937
P086-04 Structural Characterization and Biological Properties of a LipopeptideSurfactant Produced by Bacillus subtilis on Cassava Wastewater Medium
Nitschke M, Haddad R , Costa GN, Gilioli R , Meurer EC, Gatti MSV*, Eberlin MN, Höehr NF, Pastore GM
Surface-active lipopeptide compound produced by Bacillus subtilis using cassava wastewater as substrate was isolated, purified, and chemically characterized. Peptide analysis revealed presence of glutamic acid, aspartic acid, valine, and leucine. Electrospray ionization mass spectrometry showed a biosurfactant composed of homologous lipopeptide mixture, with that of molecular weight 1035 being the most abundant. Tandem mass spectrometry of each protonated molecules established the peptide sequence as R-Glu-Leu-Leu-Val-Asp-Leu-Leu. Isolated surfactant showed similar chemical composition and peptide sequence as that of commercial surfactin. Preliminary biological evaluation demonstrated surfactant has potential as antiviral agent with broad spectrum of antibacterial activity.
Food Science and Biotechnology 13: 591-596, 2004.
P087-04 Detection of Bovine Respiratory Syncytial Virus in experimentally infected balb/c mice
Almeida RS, Domingues HG, Coswig LT, D`Arce RCF, Carvalho RF, Arns CW*
An RT-nested-PCR and an immunohistochemistry assay were used to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. Chicken Embryo Related cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. The infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment, probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Only three of the lungs collected 5 days post-infection were positive by immunohistochemistry. To the author’s knowledge, this is the first study reporting bovine respiratory syncyial virus detection in balb/c mice after experimental inoculation.
Veterinary Research 35: 189-197, 2004. IF = 1.991
P088-04 Placenta cryosections for study of the adhesion of Plasmodium falciparum-infected erythrocytes to chondroitin sulfate A in flow conditions
Avri, M, Traore B, Costa F,* Lepolard C, Gysin J
Eritrócitos infectados por Plasmodium falciparum (PfEI) são capazes de aderir a superfície do endotélio provocando o impedimento do fluxo sanguíneo e a produção de citocinas inflamatórias. Mulheres de regiões endêmicas apesar de após anos de exposição ao parasita desenvolvem uma imunidade contra a doença, entretanto foi observado que na primeira gestação elas são altamente susceptíveis a infecção por P. falciparum e que os PfEI são capazes de aderir fortemente à placenta provocando distúrbios para a mãe e o feto, esta síndrome é denominada malária gestacional (MM). Também foi observado que a adesão do parasita na placenta ocorria via o domínio DBL-3 da proteína de superfície de membrana do eritrócito infectado a condroítina sulfato-A presente na placenta. Recentemente mostramos que a imunização com um recombinante baseado no domínio DBL-3 foi capaz de induzir anticorpos protetores e camundongos e primatas não humanos, abrindo excelentes perspectivas no desenvolvimento de uma vacina contra MM. Neste trabalho desenvolvemos um sistema para avaliar a capacidade de soros ou moléculas em inibir a adesão do parasita em cortes histológicos de placenta levando em consideração a pressão de fluxo encontrada na corrente sanguínea.
Microbes and Infection 6: 245-255, 2004. IF = 3.753
P089-04 Protective T cell response against malaria liver stage after vaccination with live sporozoites under chloroquine treatment
Belnoue E, Costa F*, Frankenberg T, Vigario AM, Voza T, Leroy N, Rodrigues MM, Landau I, Snounou G, Rénia L
Os efeitos de imunizações baseadas na inoculação de camundongos com formas esporozoítas de Plasmodium yoelii seguido do tratamento com cloroquina, a fim de inibir o desenvolvimento de formas sangüíneas do parasita e impedir o desenvolvimento dos sintomas da doença foram aqui estudados. Este protocolo de imunização apresentou níveis de proteção superiores aos observados pelo método utilizando esporozoítas irradiados, já descrito e estudado amplamente, que apesar de não ser utilizado como vacina convencional é capaz de gerar uma resposta protetora em camundongos, primatas e humanos. Foi mostrado que no protocolo de esporozoítas intactos seguido de tratamento diário com cloroquina a quantidade de parasitas necessária para 100% era inferior quando comparada àquela utilizada no protocolo de esporozoítas irradiados. Além disto, no protocolo testado a imunidade protetora era dependente da permanência do parasita no fígado, de células T CD4 e CD8 via a produção de óxido nítrico e interferon-gama e, independente da produção de anticorpos.
Journal of Immunology 172: 2487-2495, 2004. IF = 6.486
P090-04 Thymic Atrophy In Infectious Diseases
Verinaud LMC*, Souto PCS, Brito VN
The thymus is a primary lymphoid organ that plays an important role in the development of the immune system and in the differentiation and maturation of the vast majority of the T cell repertoire. During the normal lifespan, this organ undergoes involution in situations such as pregnancy, aging and in the presence of a wide variety of infectious diseases. This atrophy is characterized by intense structural and morphological alterations associated with an increased level of apoptosis. The fact that the thymus is a target for infectious diseases suggests that this organ provides a propitious environment for the maintenance or enhancement of infection.
Brazilian Journal of Morphological Sciences 21: 111-116, 2004.
P091-04 Morphological and intracellular alterations induced by Serratia marcescens cytotoxin
Carbonell GV, Falcón R, Yamada AT, Fonseca BAL, Yano T*
In the present work, in vitro assays used to investigate the toxicity of Serratia marcescens cytotoxin in cultured Chinese hamster ovary (CHO) cells. The time necessary to detected cellular alterations such as the onset of apoptosis, the pertubation of mitochondrial function, and cytoskeletal changes was assessed. The internalization of the cytotoxin by CHO cells was also examined. Within 10-15 min of exposure to cytotoxin, CHO cells became round, the nucleus shrank, the chromatin became more compact, and cytoplasmic blebs appeared on the cell surface. TUNEL and propidium iodide staining identified some nuclei with fragmented DNA, and electrophoresis of CHO cell DNA obtained after 30 min exposure to S.marcescens toxin showed a pattern of DNA fragments typically associated with apoptosis. The cells also lost their characteristics actin organization within 10 min of exposure to cytotoxin. Lactate dehydrogenase leakage was detected after 20 min exposure to the cytotoxin and increased with time thereafter. Concomitantly, there was a time dependent reduction in mitochondrial activity. Fluorescein-labeled S.marcescens cytotoxin was detected only on the surface of CHO cells, even after 30 min exposure to the toxin. These results show that there was no internalization of the toxin by CHO cells, and that, once bound to the cell surface, the toxin was able to induce changes in intracellular metabolism and to trigger cell death by apoptosis.
Research in Microbiology 155: 25-30, 2004. IF = 2.301
P092-04 Bacteriophages and insertion sequences of Chromobacterium violaceum ATCC 12472
Almeida RD, Trevilato PB, Bartoleti LA, Proençamodena JL, Hanna ES, Gregoracci GB, Brocchi M.*
A fluid genome is a great advantage to prokaryotes, enabling quick adaptation to various types of ecological niches and to diverse environmental selective pressures. A substantial portion of these sudden changes is mediated by lateral gene transfer (LGT), through genetic recombination mechanisms. The recent sequencing of several organisms has offered a new approach to the study of LGT, using comparison and analysis of nucleotide sequences dispersed throughout the genome of these species. This analysis in Choromobacterium violaceum revealed four prophage and 12 insertion sequences, suggesting genetic exchange with several other bacterial species. An Rhs (recombination hot spot) element (containing a vgr-like gene) was also observed, the function of which remains unknown, but it has a sequence related to species of Acinetobacter and Sphingomonas. The results support the role of LGT in the acquisition of new traits by C. violaceum.
Genetics and Molecular Research 3: 76-84, 2004.
P093-04 Drug resistance in Chromobacterium violaceum
Garboggini FF, Almeida R, Portilo VA, Barbosa TAP, Trevilato PB, Neto CER, Coelho RD, Silva DW, Bartoleti LA, Hanna ES, Brocchi M*, Manfio GP
Chromobacterium violaceum is a free-living bacterium commonly found in aquatic habitats of tropical and subtropical regions of the world. This bacterium is able to produce a large variety of products of biotechnological and pharmacological use. Although C. violaceum is considered to be non-pathogenic, some cases of severe infections in humans and other animals have been reported. Genomic data on the type strain ATCC 12472(T) has provided a comprehensive basis for detailed studies of pathogenicity, virulence and drug resistance genes. A large number of open reading frames associated with various mechanisms of drug resistance were found, comprising a remarkable feature of this organism. Amongst these, beta-lactam (penicillin and cephalosporin) and multidrug resistance genes (drug efflux pumps) were the most numerous. Genes associated with bacitracin, bicyclomycin, chloramphenicol, kasugamycin, and methylenomycin were also found. It is assumed that these genes contribute to the ability of C. violaceum to compete with other bacteria in the environment, and also may help to explain the common drug resistance phenotypes observed in infections caused by this bacterium.
Genetics and Molecular Research 3: 134 -147, 2004.
P094-04 Virulence characteristics and epidemiology of Yersinia enterocolítica and Yersinia other than Y. pseudotuberculosis and Y. pestis isolated from water and sewage
Falcão JP, Brocchi M*, Proença-Modena JL, Acrani GO, Correa EF, Falcão DP
To determine the species, bio-sero-phagetypes, antimicrobial drug resistance and also the pathogenic potential of 144 strains of Yersinia spp. isolated from water sources and sewage in Brazil. The strains were characterized biochemically, serologically and had their antibiotic resistance and phenotypic virulence markers determined by microbiological and serological standard techniques. The 144 isolates belonged to various bio-serogroups. Half of the strains showed resistance to three or more drugs. The Y. enterocolitica strains related to human diseases exhibited phenotypic virulence characteristics and virulence genes. Water from various sources and sewage are contaminated with Yersinia spp. in Brasil. Among these bacteria, virulent strains of Y. enterocolitica were found, with biotypes and serogroups related to human diseases. This is the first documented description of the occurrence of pathogenic Y. enterocolitica in water sources and sewage in Brazil. The occurrence of virulence strains of Y. enterocolitica shows that the environment is a potential source of human infection by this species in this country.
Journal of Applied Microbiology 96: 1230-1236, 2004. IF = 1.835
P095-04 Effect of media composition on Interleukin-2 production by murine El-4 thymoma cells
Galesi ALL, Tamashiro WMSC*, Moraes AM
Due to the role of interleukin-2 (IL-2) in the mediation of immune response, this cytokine has been used in the treatment of some types of cancer and infectious diseases. However, relatively high levels of this cytokine are required to achieve significant activity. The aim of this work was to study a culture medium composition designed to increase the production of IL-2 by suspended murine EL-4 cells. The cultivations were carried out aiming at producing IL-2 in stirred bioreactors. The effects of concentration of glutamine, phorbol-12-myristate-13-acetate (PMA), concanavalin A (Con A), Pluronic F68, and fetal calf serum (FCS) on cell viability and IL-2 production were evaluated. PMA alone was more efficient in IL-2 production than it was in association with Con A. The maximum IL-2 production was around 162 ng/mL with 856 ng/mL PMA and 1.45% (v/v) FCS.
Brazilian Journal of Chemical Engineering 21: 165-173, 2004. IF = 0.212
P096-04 Induction of systemic tolerance in normal but not in transgenic mice through continuous feeding of ovalbumin
Simioni PU, Fernandes LGR, Gabriel DL, Tamashiro WMSC.*
The ingestion of most dietary protein can cause systemic tolerance, and such tolerance is easier to induce in younger than in older mice. In this study, we examined whether oral tolerance to ovalbumin (OVA) could be induced in OVA-T-cell receptor (OVA-TCR)-specific transgenic mice. Continuous feeding or gavage with OVA induced tolerance, measured as reduced antibody production, in young and aged BALB/c mice, in a dose-dependent manner, but this effect was not observed in transgenic mice. DO11.10 mice did not become tolerant upon continuous feeding with OVA, and the adoptive transfer of naý ve cells increased the levels of specific antibodies in their sera after antigenic challenge. The immunization schedule used here leads to a Th2-dependent antibody response in normal BALB/c mice. However, the same schedule induced both Th1- and Th2-antibody responses in transgenic mice. Transforming growth factor (TGF)-b secretion was significantly lower in the supernatants of T cells from both normal and transgenic mice cocultured with DC from DO11.10 mice that had consumed OVA, while it was significantly higher in the presence of DC from normal tolerant mice, thus implicating TGF-b as a regulatory cytokine in oral tolerance in the murine model.
Scandinavian Journal of Immunology 60: 257-266, 2004. IF = 1.912
P097-04 Suppression of nitric oxide production in mouse macrophages by soybean flavonoids accumulated in response to fungal elicitation
Scuro LS, Simioni PU, Gabriel DL, Saviani EE, Modolo LV, Tamashiro WMSC*, Salgado I
The anti-inflammatory properties of some flavonoids have been attributed to their ability to inhibit the production of NO by activated macrophages. Soybean cotyledons accumulate certain flavonoids following elicitation with an extract of the fungal pathogen Diaporthe phaseolorum f. sp. meridionalis (Dpm). Sodium nitroprusside (SNP), a nitric oxide donor, can substitute for Dpm in inducing flavonoid production. In this study, we investigated the effect of flavonoid-containing diffusates obtained from Dpm- and SNP-elicited soybean cotyledons on NO production by lipopolysaccharide (LPS)- and LPS plus interferon-γ (IFNγ)-activated murine macrophages. Significant inhibition of NO production, measured as nitrite formation, was observed when macrophages were activated in the presence of soybean diffusates from Dpm- or SNP elicited cotyledons. This inhibition was dependent on the duration of exposure to the elicitor. Daidzein, genistein, luteolin and apigenin, the main flavonoids present in diffusates of elicited cotyledons, suppressed the NO production by LPS + IFNγ activated macrophages in a concentration-dependent manner. iNOS expression was not affected by daidzein, was reduced by genistein, and was abolished by apigenin, luteolin and Dpm and SNP-soybean diffusates at concentrations that significantly inhibited NO production by activated macrophages. These results suggest that the suppressive effect of flavonoids on iNOS expression could account for the potent inhibitory effect of Dpm- and SNP-diffusates on NO production by activated macrophages. The treatment of soybean tissues with SNP may provide a simple method for substantially increasing the concentration of metabolites that are beneficial for the treatment of chronic inflammatory diseases associated with NO production.
BCM Biochemistry. Biomed Central Biochemistry: 5,5 , 2004.
P098-04 Inhibition of 5-alpha-reductase activity induces stromal remodeling and smooth muscle de-differentiation in adult gerbil ventral prostate
Corradi LS, Carvalho HF*, Goes RM, Taboga SR et al
Differentiation 72: 198-208, 2004. IF = 4.481
P099-04 Smooth muscle cell behavior in the ventral prostate of castrated rats
Antonioli E, Della-Colleta HHM, Carvalho HF*
Journal of Andrology 25: 50-56, 2004. IF = 2.394
P100-04 Shell-size selection by intertidal sympatric hermit crabs
Turra A, Leite FPP*
Marine Biology 145: 251-257, 2004. IF = 1.772
P101-04 Composition of the Lecointea clade (Leguminosae, Papillionideae, Swartzieae), a re-evaluation based on combined evidence from morphology and molecular data
Mansano VD, Bittrich V, Tozzi AMCD, de Souza AP*
Taxon 53: 1007-1018, 2004. IF = 1.752
P102-04 Chromosomal studies of species of Rubiaceae (A. L. de Jussieu) from the Brazilian cerrado
Correa AM, Forni-Martins ER*
Caryologia 57: 250-258, 2004. IF = 0.366
P103-04 A triploid cytotype of Echinodorus tennellus
Costa MY, Forni-Martins ER*
Aquatic Botany 79: 325-332, 2004. IF = 1.143
P104-04 Chromosomal and morphological studies of diploid and polyploid cytotypes of Stevia rebaudiana (Bertoni) Bertoni (Eupatorieae, Asteraceae)
Oliveira VM, Forni-Martins ER*, Magalhães PM, Alves MN
Genetics and Molecular Biology 27: 215-222, 2004. IF = 0.316
P105-04 Synanthropy of Muscidae (Diptera) in the city of Valdivia, Chile
Figueroa-Roa L, Linhares AX*
Neotropical Entomology 33: 647-651, 2004.
P106-04 Sperm ultrastructure of the Brazilian Amazon poison frogs Epipedobates trivittatus and Epipedobates hahneli (Anura, Dendrobatidae)
Aguiar-Jr 0, Lima AP, Báo SN, Recco-Pimentel SM*
The sperm ultrastructure of Epipedobates trivittatus and E. hahneli is described. The spermatological characteristics are identical in both species, except for the extension of the acrosomal vesicle. The structure of the acrosomal complex is shared with species of the Bufonoidea neobatrachian lineage, which differs from the condition observed to species included into the ranoid lineage. The perpendicular arrangement of the centrioles is also shared with the Bufonoidea, and is considered a synapomorphic condition. An expanded undulating membrane and the absence of a juxtaxonemal fiber appear to be apomorphic characteristics of dendrobatid species, as also observed for Litoria and Cyclorana species. However, the notable expansion of the undulating membrane appears to be an autapomorphy of Dendrobatidae frogs. The ultrastructural data presented here do not support the proposed placement of E. trivittatus in a separate genus.
Acta Zoologica (Stockolm) 85: 21-28, 2004. IF = 1.089
P107-04 Cytogenetics of Hylodes and Crossodactylus (Anura: Hylodinae) with comments on Hylodinae/Dendrobatidae relationships
Aguiar-Jr O, Carvalho KA, Giaretta AA, Recco-Pimentel SM*
The karyotype, NOR location and C-banding pattern of two species of Hylodes (H. phyllodes and H. asper), and two of Crossodactylus were described. The results from the conventionally stained karyotypes confirm the uniformity within the genus Crossodactylus as well as the relatively conserved karyotypes within Hylodes, in agreement with the already indicated in the literature. We conclude that the cytogenetic data weakly supports the relationships between hylodines and dendrobatids, since no unumbiguous homeologies can be recognized between the karyotypes of these groups.
Genetica 121: 43-53, 2004. IF = 2.085
P108-04 Cytogenetic analysis of three species of Eleutherodactylus (Leptodactylidae, Anura) from southeastern Brazil
Siqueira-Jr S, Ananias F, Recco-Pimentel, SM*
In this paper, we provide a cytogenetic analysis of Eleutherodactylus guentheri, E. parvus and E. binotatus. All of the species had a diploid chromosome number of 2n=22. The karyotypes of E. guentheri and E. parvus were very similar, and differed only slightly in the morphology of pair 2. These two species also had a NOR-bearing secondary constriction on the long arms of pair 6. The karyotype of E. binotatus differed from those of E. guentheri and E. parvus in the morphology and size of the chromosomes, in the number of chromosome arms, in the NOR location, and in the pattern of heterochromatin. These results reinforce the differences between E. guentheri and E. binotatus and give support to the existence of two species group. Five individuals of E. binotatus showed morphs for pairs 2 and 3. These morphs probably arose from the translocation of a segment from one chromosome of pair 3 to one homologue of pair 2. In addition, some mitotic metaphases of E. binotatus showed spontaneous chromosomal breaks which suggested that there were sites of fragility. Meiotic diakinesis showed multiple chromosomal rings, indicating the occurrence of multiple translocations, as previously reported by other investigators. These data suggest that, in addition to fission and fusion, other chromosomal rearrangements were probably involved in the differentiation of the karyotypes of these species of Eleutherodactylus, especially E. binotatus.
Genetics and Molecular Biology 27: 363-372, 2004. IF = 0.316
P109-04 Conserved karyotypes in Hyla pulchella species group (Anura: Hylidae)
Ananias F, Garcia PC.A, Recco-Pimentel SM*
Cytogenetic analyses were done on specimens of H. marginata and on three populations of H. semiguttata differing in morphology and in the physical parameters of their advertising call, as well as in individuals of Hyla sp. (aff. semiguttata). All specimens had 2n = 24 chromosomes with a morphology very similar to that of other 24-chromosome Hyla. Hyla semiguttata and H. marginata showed the same C-banding pattern but were distinguished by the location of the NOR on pair 1 in H. semiguttata and Hyla sp. (aff. semiguttata), and on pair 10 in H. marginata. The H. semiguttata populations did not differ cytogenetically, despite variations in their morphology and advertisement calls. Similarly, H. semiguttata and H. p. joaquini had identical C-banding patterns and NOR locations, suggesting that they are very closely related.
Hereditas 140: 42-48, 2004. IF = 0.690
P110-04 Chromosomal characterization of H. bischoffi bischoffi and Hyla guentheri (Anura, Hylidae)
Raber SC, Garcia PC, Vinciprova G, Recco-Pimentel SM*
Hyla bischoffi and H. guentheri share some morphological and call characteristics with the H. pulchella and H. polytaenia groups. The inclusion of these two species in one of these two groups is still controversial. A cytogenetic study showed that both species have 2n = 24 chromosomes, consisting of five metacentric, five submetacentric and two subtelocentric chromosome pairs. The nucleolus organiser region (NOR) was located on the long arm of chromosome 10, which also presented a block of heterochromatin in both species. The pericentromeric region of all the chromosomes were positively C-banded. There were no conspicuous differences in the karyotypes of these two species, except for an additional heterochromatic C-band on the short arms of chromosome 6 in H. bischoffi. The karyotypes of these two species were very similar to those of the H. pulchella group and indicated that neither species can be excluded from that group.
Phyllomedusa 3: 43-49, 2004.
P111-04 Spermatozoa morphology of Brachidontes darwinianus and B. solisianus (Bivalvia, Mytilidae) from the southern Brazilian coast
Introíni GO, Aguiar-Jr O, Quaresma AJC, Lino-Neto J, Magalhães CA, Recco-Pimentel SM*
We used transmission and scanning electron microscopy to study the structure of mature spermatozoa from two bivalves, Brachidontes darwinianus and B. solisianus, and compared them with those of other bivalves, particularly other mytilids. The spermatozoa of both species were of the primitive type or ect-aquasperm form. In both species, the spermatozoan head contained an spheroidal nucleus capped by a conical acrosome with an anterior extension. Immunocytochemical staining identified actin throughout the nucleus of the sperm of both species. The chromatin was strongly electron-dense, homogenous and compact. The mid-piece region consisted of five spherical mitochondria grouped in a ring around a pair of short cylindrical centrioles. The flagellum exhibited the typical 9+2 microtubule structure. These findings, together with conchological characteristics, can be used to distinguish between B. darwinianus and B. solisianus. The only marked difference in the morphology of spermatozoa from these two species was the longer anterior extension of the acrosomal vesicle in B. solisianus. This elongated acrosome may facilitate penetration of the jelly coat and cytoplasm of large oocytes and could increase the efficiency of fertilization with a resulting enhanced reproductive success which could account for the wider geographic distribution of B. solisianus.
Invertebrate Reproduction & Development 46: 149-158, 2004. IF = 0.633
P112-04 Beneficial effects of flower-dwelling predators on their host plant
Romero GQ, Vasconcellos-Neto J*
Ecology 85: 446-457, 2004. IF = 4.104
P113-04 Foraging by the flower-dwelling spider, Misumenops argenteus (Thomisidae), at high prey density sites
Romero GQ, Vasconcellos-Neto J*
Journal of Natural History 38: 1287-1296, 2004. IF = 0.514
P114-04 Exposure to larva migrans syndromes in squares and public parks of cities in Chile
Marcado R, Ueta MT*, Castillo D, Munoz V, Schenone H
Revista de Saúde Pública 38: 729-731, 2004.
P115-04 Pollination biology and breeding system of Zeytheria Montana (Bignoniaceae)
Bittencourt NS, Semir J*
Plant Systematics and Evolution 247: 241-254, 2004. IF = 1.338
P116-04 A new species of Minasia (Asteraceae, Vernonieae) from the Planalto de Diamantina, Minas Gerais, Brazil
Semir J*, Jesus FF
Novon 14: 233-235, 2004. IF = 0.231
P117-04 Lip anatomy and its implications for the pollination mechanisms of Bulbophyllum species (Orchidaceae)
Teixeira SD, Borba EL, Semir J*
Annals of Botany 93: 499-505, 2004. IF = 2.262
P118-04 Histochemical and ultrastructural study of collagen fibers in mouse pubic symphysis during late pregnancy
Pinheiro MC, Moraes SG, Battlehner CN, Caldini EG, Toledo EMS, Joazeiro PP*
Mícron 35: 685-693, 2004. IF = 1.464
P119-04 Phenotypic modulation of fibroblastic cells in mice pubic symphysis during pregnancy, partum and postpartum
Moraes SG, Pinheiro MC, Toledo OMS, Joazeiro PP*
Cell and Tissue Research 315: 223-231, 2004. IF = 2.670
P120-04 Carbon fixation and partitioning in coffee seedlings infested with Pratylenchus coffeae
Mazzafera P*, Kubo RK, Inomoto MM
European Journal of Plant Pathology 110: 861-865, 2004. IF = 1.384
P121-04 Distribution of bioactive substances from Hypericum brasiliense during plant growth
Abreu IN, Porto ALM, Marsaioli AJ, Mazzafera P*
Plant Science 167: 949-954, 2004. IF = 1.389
P122-04 Plant biochemistry – A naturally decaffeinated arábica coffee
Silvarolla MB, Mazzafera P*, Fazuoli LC
Nature 429: 826, 2004. IF = 32.182
P123-04 Catabolism of caffeine in plants and microorganisms
Frontiers in Bioscience 9: 1348-1359, 2004. IF = 3.226
P124-04 Essential oil composition of Hypericum brasiliense choise
Abreu LN, Reis MG, Marsaioli AJ, Mazzafera P*
Flavour and Fragrance Journal 19: 80-82, 2004. IF = 0.623
P125-04 Spatial structure of Aspidosperma polyneuron in two semi-deciduous forests in southeast Brazil
Fonseca MG, Martini AMZ, dos Santos FAM*
Journal of Vegetation Science 15: 41-48, 2004. IF = 1.845
P126-04 Effects of anoxia on root ultrastructure of four neotropical trees
Kolb RM, Dolder H, Cortelazzo AL*
Protoplasma 224: 99-105, 2004. IF = 1.625
P127-04 Mapping QTLs for kernel oil content in a tropical maize population
Mangolim CA, de Souza CL, Garcia AAF, Garcia AF, Sibov ST, de Souza AP*
Euphytica 137: 251-259, 2004. IF = 0.797
P128-04 Diet of Alouatta belzebul discolor in na Amazonian rain Forest of northern Mato Grosso state, Brazil
Pinto LP, Setz EZF*
International Journal of Primatology 25: 1197-1211, 2004. IF = 1.326
P129-04 Daytime hunting behaviour of Echidna catenata (Muiraenidae): why chain morays foragging at ebb tide have no followers
Sazima I*, Sazima C
Aqua Journal of Ichthyology and Aquatic Biology 8: 1-8, 2004.
P130-04 Vampire catfishes seek the aorta not the jugular: candirus of the genus Vandellia (Trichomycteridae) feed on major gill arteries of host fishes
Zuanon J, Sazima I*
Aqua Journal of Ichthyology and Aquatic Biology 8: 31-36, 2004.
P131-04 A new species of Scinax (Anura: Hylidae) from rocky montane fields of the Serra do Cipó, Southeastern Brazil
Pugliese A, Pombal Jr JP, Sazima I*
Zootaxa 688: 1-15, 2004.
P132-04 Natural history of Trichogenes longipinnis, a threatened trichomycterid catfish endemic to Atlantic forest streams in southeast Brazil
Ichthyological Exploration of Freshwaters 15: 49-60, 2004.
P133-04 The moving gardens: reef fishes grazing, cleaning, and following green turtles
Sazima C, Grossmann A, Bellini C, Sazima I*
Cybium 28: 47-53, 2004. IF = 0.312
P134-04 Hawksbill turtles visit moustached barbers: cleaning symbiosis between Eretmochelys imbricata and the shrimp Stenopus hispidus
Sazima I*, Grossman A, Sazima C
Biota neotropica 4: 1-6, 2004.
P135-04 A new species of Physalaemus (Amphibia: Leptodactylidae) from the Atlantic forest in southeast Brazil
Haddad CFB, Sazima I*
Zootaxa 479: 1-12, 2004.
P136-04 Chirocentrodon bleekerianus (Teleostei: Clupeifomes: Pristigasteridae), a small predaceous herring with folded and distinctively oriented prey in stomach
Sazima C, Moura RL, Sazima I*
Brazilian Journal of Biology 64: 165-168, 2004.
P137-04 First record of Polyprion oxygeneios (Perciformes: Polyprionidae) for the south-west Atlantic and a northenmost range extension
Barreiros JP, Machado L, Hostim-Silva M, Sazima I*, Heemstra PC
Journal of Fish Biology 64: 1439-1441, 2004. IF = 1.198
P138-04 Trichomycterus maracaya, a new catfish from the upper rio Paraná, southeastern Brazil (Siluriformes: Trichomycteridae), with notes on the T. brasiliensis species-complex
Bockmann FA, Sazima I*
Neotropical Ichthyology 2: 61-74, 2004.
P139-04 Does the association of young fishes with jellyfishes protect from predation?
Bonaldo RM, Krajewski JP, Sazima I*
Neotropical Ichthyology 2: 103-105, 2004.
P140-04 The association of the goatfish Mulloidichthys martinicus with the grunt Haemulon chrysargyreum: an example of protective mimicry
Krajewski JP, Bonaldo RM, Sazima C, Sazima I*
Biota neotropica 4: 1-4, 2004.
P141-04 Vomiting behavior of the spinner dolphin (Stenella longirostris) and squid meals
Silva Jr JM, Pandolfo LJ, Sazima I*
Aquatic Mammals 30: 271-274, 2004.
P142-04 Rough-toothed dolphins (Steno bredanensis) catching diskfishes while interacting with humpback whales (Megaptera novaeangliae) of Abrolhos bank breeding ground, Brazil
Wedekin LL, Freitas A, Engel MH, Sazima I*
Aquatic Mammals 30: 327-329, 2004.
P143-04 Natural history of Stauroglanis gouldingi (Siluriformes: Trichomycteridae), a miniature sand-dwelling candiru from central Amazonia streamlets
Zuanon J, Sazima I*
Ichthyological Exploration of Freshwaters 15: 201-208, 2004.
P144-04 Octopus cleaned by two fish species at Fernando de Noronha Archipelago, SW Atlantic
Sazima I*, Krajewski JP, Bonaldo RM, Sazima C
Coral Reefs 23: 484, 2004. IF = 1.828
P145-04 A non-fatal attack by the tiger shark, Galeocerdo cuvieri, on the Northeast coast of Brazil (Chondrichthyes: Catcharhinidae)
Gadig OBF, Sazima I*
Arquivo de Ciências do Mar 36: 119-122, 2004.
P146-04 Allometry of a neotropical palm, Euterpe edulis Mart.
Alves LF, Martins FR, Santos FAM*
Acta Botanica Brasilica 18: 369-374, 2004.
P147-04 Tree mortality in a riparian Forest at Rio Paraguai, Pantanal, Brazil, after na extreme flooding
Damasceno Jr GA, Semir J, dos Santos FAM*, Leitão Filho HF
Acta Botanica Brasilica 18: 839-846, 2004.
P148-04 Sucessão ecológica da vegetação arbórea em uma floresta estacional semidecidual, Viçosa, MG, Brasil
Paula A, Silva AF, de Marco Jr P, dos Santos FAM*, Souza AL
Acta Botanica Brasilica 18: 407-423, 2004.
P149-04 Aspects of arbuscular mycorrhizal fungi in na Atlantic Forest chronosequence – Parque Estadual Turístico do Alto Ribeira (PETAR), SP
Aidar MPM, Carrenho R, Joly CA*
Biota Neotropica 4: online, 2004.
P150-04 Quantas espécies de Drosophila (Díptera, Drosophilidae) ainda não foram descritas nas florestas de São Paulo, Brasil? Listas de espécies de três remanescentes florestais
Medeiros HF, Klaczko LB*
Biota neotropica 4 (1) online, 2004.
P151-04 Comparison of RAPD, RFLP, AFLP and SSR markers for diversity studies in tropical maize inbred lines
Garcia AAF, Benchimol LL, Barbosa AMM, Geraldi IO, Souza CL, de Souza AP*
Genetics and Molecular Biology 27: 579-588, 2004. IF = 0.316
P152-04 Survey in the sugarcane expressed sequence tag database (SUCEST) for simple sequence repeats
Pinto LR, Oliveira KM, Ulian EC, Garcia AAF, de Souza AP*
Genome 47: 795-804, 2004. IF = 2.100
P153-04 Estimates of genetic variance and level of dominance in a tropical maize population. I. Grain yield and plant traits
Silva AR, Souza CL, Aguiar AM, de Souza AP*
Maydica 49: 65-71, 2004. IF = 0.600
P154-04 Evaluating genetic relationships between tropical maize inbred lines by means of AFLP profiling
Oliveira KM, Laborda PR, Garcia AAF, Paterniani MEAGZ, de Souza AP*
Hereditas 140: 24-33, 2004. IF = 0.690
P155-04 Comparison of similarity coefficients used for cluster analysis with dominant markers in maize (Zea mays L.)
Meyer AD, Garcia AAF, de Souza AP*, de Souza CL
Genetics and Molecular Biology 27: 83-91, 2004. IF = 0.316
P156-04 Cloning, expression, and purification of the virulence-associated protein D from Xylella fastidiosa
Catani CF, Azzoni AR, Paula DP, Tada SFS, Rosselli LK, de Souza AP, Yano T*
Protein Expression and Purification 37: 320-326, 2004. IF = 1.336
P157-04 Spatial distribution patterns of jumoing spiders associated with terrestrial bromeliads
Romero GQ, Vasconcellos-Neto J*
Biotropica 36: 596-601, 2004. IF = 0.977
P158-04 Tribal and intergeneric relationships of Mesachitea (Apocynoideae, Apocynaceae): evidence from three noncoding plastid DNA regions and morphology
Simões AO, Endress ME, van der Niet T, Kinoshita LS*, Conti E
American Journal of Botany 91: 1409-1418, 2004. IF = 2.438
P159-04 Evaluation by blue native polyacrylamide ekectrophoresis colorimetric staining of the effects of physical exercise on the activities of mitochondrial complexes in rat muscle
Molnar AM, Alves AA, Pereira-da-Silva L, Macedo DV*, Dabbeni-Sala F
Brazilian Journal of Medical and Biological Research 37: 939-947, 2004. IF = 0.824
P160-04 Free 2-propen-1-amine derivative and inclusion complexes with beta-cyclodextrin: scanning electron microscopy, dissolution, cytotoxicity and antimycobacterial activity
De Souza AO, Santos RR, Sato DN, Azevedo MMM, Ferreira DA, Melo PS, Haun M*, Silva CL, Duran N
Journal of the Brazilian Chemical Society 15: 682-689, 2004. IF = 1.161
P161-04 Cytotoxicity of the phytosterol diosgenin and its derivatives in rat cultured hepatocytes and V79 fibroblasts
Melo PS, Azevedo MBM, Zullo MAT, Fabrin Neto JB, Haún M*
Human & Experimental Toxicology 23: 487-493, 2004. IF = 1.176
P162-04 Dehydrocrotonin and its derivative, dimethylamide-crotonin induce apoptosis with lipid peroxidation and activation of caspases-2,-6 and –9 in human leukemic cells H60
Anazetti MC, Melo PS, Duran N, Háun M*
Toxicology 203: 123-137, 2004. IF = 2.691
P163-04 Effect of angiotensin II and losartan on the phagocytic activity of peritoneal macrophages from Balb/C mice
Belline P, Melo PS, Haún M*, Palhares FB, Boer PA, Gontijo JAR, Figueiredo JF
Memórias do Instituto Oswaldo Cruz 99: 167-172, 2004. IF = 0.740
P164-04 Natural killer cell activity and anti-tumor effects ofdehydrocrotonin and its synthetic derivatives
Melo PS, Justo GZ, Duran N, Haún M*
European Journal of Pharmacology 487: 47-54, 2004. IF = 2.432
P165-04 Modulation of eosinophil migration from boné marrow to lungs of allergic rats by nitric oxide
Ferreira HHD, Costa RAD, Jacheta JM, Martins AR, Medeiros MV, Macedo-Soares MF, De Lucca IMS*, Antunes E, de Nucci G
Biochemical Pharmacology 68: 631-639, 2004. IF = 3.436
P166-04 Biomphalaria tenagophila: genetic variability within intermediate snail hosts susceptible and resistant to Schistosoma mansoni infection
Da Silva D, Spada RGM, Sobral-Hamaguchi SS, Abdel-Hamid Z, Zuim NRB, Zanotti-Magalhães EM, Magalhães LA*, Ribeiro-Paes JT
Parasite-Journal de la Societé Française de Parasitologie 11: 43-49, 2004. IF = 0.833
P167-04 Hypoxia modulates expression of the 70-kD heat shock protein and reduces Leishmania infection in macrophages
Degrossoli A, Colhone MC, Arrais-Silva WW, Giorgio S*
Journal of Biomedical Science 11: 847-854, 2004. IF = 1.567
P168-04 Effect of hypoxia on macrophage infection by Leishmania amazonensis
Colhone MC, Arrais-Silva WW, Picoli C, Giorgio S*
Journal of Parasitology 90: 510-515, 2004. IF = 1.439
P169-04 Biochemical and biomechanical analysis of tendons of caged and penned chickens
Benevides GP, Pimentel ER, Toyama MH, Novello JC, Marangoni S, Gomes L*
Connective Tissue Research 45: 206-215, 2004. IF = 1.149
P170-04 Pachycondyla obscuricornis as natural enemy of the spittlebug Deois flavopicta
Suji ER, Garcia MA*, Fontes EMG et al.
Pesquisa Agropecuária Brasileira 39: 607-609, 2004. IF = 0.167
P171-04 Overexpression, purification and characterization of Gum C, na enzyme involved in the biosynthesis of exopolysaccharide by Xylella fastidiosa
De Pieri C, …., Arruda P* et al.
Protein Expression and Purification 34: 223-228, 2004. IF = 1.336
P172-04 Effects of a leucine-rich diet on body composition during nutritional recovery in rats
Ventrucci G, Silva LGR, Mello MAR, Marcondes MCCG*
Nutrition 20: 213: 217, 2004. IF = 1.958
P173-04 Placental glycogen metabolism changes during walker tumour growth
Toledo MT, Marcondes MCCG*
Placenta 25: 456-462, 2004. IF = 2.683
P174-04 Occurrence of Cryptosporidium spp in small wildlife mammals from three mountainous areas in the Brazilian Southeastern region
Dall’Olio AJ, Franco RMB*
Arquivo Brasileiro de Medicina Veterinária e Zootecnia 56: 25-31, 2004. IF = 0.092
P175-04 Leaf domatia mediate mutualism between mites and a tropical tree
Romero GQ, Benson WW*
Oecologia 140: 609-616, 2004. IF = 2.899
P176-04 Intercations between and species increase arrival rates of an ant parasitoid
Orr MR, de Camargo RX, Benson WW*
Animal Behaviour 65: 1187-1193, 2003. IF = 2.092
P177-04 Ontogenesis succession in Amazonian ant trees
Fonseca CR, Benson WW*
Oikos 102: 407-412, 2003. IF = 2.901
P178-04 Ecological interactions among ants in the genus Linepithema, their phorid parasitoids, and ant competitors
Orr MR, Dahlsten DL, Benson WW*
Ecological Entomology 28: 203-210, 2003. IF = 1.568
P179-04 Overexpression, purification, biochemical charcetrization, and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa
Muniz JRC, … Arruda P*, et al.
Biochemical and Biophysical Research Communications 315: 485-492, 2004. IF = 2.904
P180-04 Pollination and reproductive biology of twelve species of neotropical malpighiaceae: Stigma morphology and its implications for the breeding system
Sigrist MR, Sazima M*
Annals of Botany 94: 33-41, 2004. IF = 2.262
P181-04 Non-Euglossine bees also fucntion as pollinators of Sinningia species (Gesneriaceae) in southeastern Brazil
SanMartin-Gajardo I, Sazima M*
Plant Biology 6: 506-512, 2004. IF = 1.582
P182-04 Hummingbird-plant interactions in three plant communities of the southeastern part of Parque Nacional Natural Chiribiquete, Colombia
Lasprilla LR, Sazima M*
Ornitologia Neotropical 15: 183-190, 2004.
P183-04 Low protein diet induced alteration in gene expression in rat pancreatic islets
Delghingaro-Augusto V, Bordin S, Ferreira F, Amaral MCE, Toyama MH, Boschero AC*, Carneiro EM
Journal of Nutrition 134: 321-327, 2004. IF = 3.245
P184-04 Decreased insulin secretion in islets from rats maintained in a low protein diet is associated to a reduced PKA expression
Ferreira F, Barbosa HCL, Pereira EA, Boschero AC*, Delghingaro-Augusto V, Stoppiglia LF, Carneiro EM
Journal of Nutrition 134: 63-67, 2004. IF = 3.245
P185-04 Efecto Del INGAP (Proteína asociada a la neogenesis insular) sobre la secreción de insulina de islotes pancreáticos de ratas adultas y recién nacidas
Borelli MI, Stoppiglia LF, Rezende LF, Flores LE, Boschero AC*, Gagliardino JJ
Revista Argentina de Endocrinologia y Metabolismo 41: 9-13, 2004.
P186-04 Restauration of insulin secretion in pancreatic islets of protein-deficient rats by antisense oligonucleotide blockade of IRSI
Araújo EP, Amaral MEC, Filiputti E, Souza CT, Laurito TL, Delghingaro-Augusto V, Saad MJA, Boschero AC*, Velloso LA, Carneiro EM
Journal of Endocrinology 181: 25-38, 2004. IF = 3.319
P187-04 Prolactin-modulated gene expression profiles in pancreatic islets from adult female rats
Bordin S, Amaral MEC, Anhê GF, Delghingaro-Augusto V, Cunha DA, Nicoletti-Carvalho JE, Boschero AC*
Molecular and Cellular Endocrinology 200: 41-50, 2004. IF = 2.626
P188-04 Characterization of the peroxidase system of neonatal rat isolated islets at low H2O2 concentrations
Stoppiglia LF, Rezende LF, Ferreira F, Filiputti E, Carneiro EM, Boschero AC*
Biochimica et Biophysica Acta 1690: 159-168, 2004. IF = 2.557
P189-04 Participation of prolactin receptors and PI3K and MAPK pathways in the increase in pancreatic islet mass and sensitivity to glucose during pregnancy
Amaral MEC, Cunha DA, Anhê GF, Ueno M, Carneiro EM, Velloso LA, Bordin S, Boschero AC*
Journal of Endocrinology 183: 469-476, 2004. IF = 3.319
P190-04 Upregulation of the expression of tight and adherens junction-associated proteins during maturation of neonatal pancreatic islets in vitro
Collares-Buzato CB, Carvalho CPF, Furtado AG, Boschero AC*
Journal of Molecular Histology 35: 811-822, 2004. IF = 0.928
P191-04 Ocorrência de zoocistos de Cryptosporidium spp e cistos de Giardia spp em diferentes pontos do processo de tratamento de água em Campinas, São Paulo, Brasil
Franco RMB*, Cantúsio Neto R
Revista Higiene Alimentar 18: 52-59, 2004.
P192-04 Occurrence of Giardia cysts and Cryptosporidium oocysts in activated sludge in Campinas, São Paulo, Brazil
Franco RMB*, Santos LU, Bonatti TR, Cantúsio Neto R
Revista do Instituto de Medicina Tropical de São Paulo 46: 309-313, 2004.
P193-04 Enzyme activities associated with oxidative stress in Metarhizium anisopliae during germination mycelial growth and conidiation and in response to near-UV irradiation
Messias CL, Braga GUL, Miller CD, + 5 authors
Canadian Journal of Microbiology 50: 41-49, 2004. IF = 1.118
P194-04 Effect of naproxen on tail fin regeneration in teleost
Bockelmann PK, Bechara IJ*
Journal of Submicroscopic Cytology and Pathology 36: 1-10, 2004.
P195-04 Book review: Cycads of the world
Plant Systematics and Evolution: 244: 123-125, 2004. IF = 1.338
P196-04 Sexual mimicry in Mormolyca ringens Lindl. Schltr. (Orchidaceae: Maxillariinae)
Singer RB, Flach A, Koehler S, Marsaioli AJ, Amaral MCE*
Annals of Botany 93: 755-762, 2004. IF = 2.262
P197-04 The chemistry of pollination in selected Brazilian Maxillariinae orchids: floral rewards and fragance
Flach A, Dondon R, Singer RB, Koehler S, Amaral MCE*, Marsaioli AJ
Journal of Chemical Ecology 30: 1045-1056, 2004. IF = 1.607
P198-04 Steroids and triterpenoids from Eleocharis acutangula and E. sellowiana (Cyperaceae)
Amaral MCE*, Faria AD, Magalhães AF, Magalhães EG, Ruiz ALTG
Phytochemical Analysis 15: 125-129, 2004. IF = 1.385
P199-04 Pollinator attraction devices (floral fragances) of some Brazilian orchids
Reis MG, Pansarin ER, Silva UF, Amaral MCE*, Marsaioli AJ
ARKIVOC 6: 89-97, 2004. IF = 0.418
P200-04 A taxonomic study of the South American genus Bifrenaria Lindl. (Orchidaceae)
Koehler S, Amaral MCE*
Brittonia 56: 314-345, 2004. IF = 0.222
P201-04 Attaching and effacing Escherichia coli isolated from dogs in Brazil: characteristics and serotypic relationship to human enteropathogenic E. coli (EPEC)
Nakazato G, …, Silveira WD*, Castro AFP
Veterinary Microbiology 101: 269-277, 2004. IF = 1.930
P202-04 New species of Tomoplagia Coquillett (Diptera: Tephritidae) from capitula of Asteraceae in Brazil
Prado PI, Norrbom AL, Lewinsohn TM*
Neotropical Entomology 33: 189-211, 2004.
P203-04 Compartments in insect-plant associations and their consequences for community structure
Prado PI, Lewinsohn TM*
Journal of Animal Ecology 73: 1168-1178, 2004. IF = 3.342
P204-04 Geographical distribution of Eupatorieae (Asteraceae) in South-eastern and South Brazilian Mountain ranges
Almeida AM, Prado PI, Lewinsohn TM*
Plant Ecology 174: 163-181, 2004. IF = 1.275
P205-04 Small-scale spatial autocorrelation and the interpretation of relationships between phenological parameters
Almeida-Neto M, Lewinsohn TM*
Journal of Vegetation Science 15: 561-568, 2004. IF = 1.845
P206-04 Physiological and microbiological aspects of fresh cut beet roots
Vitti MCD, Kluge RA, Gallo CR, Schiavinato MA*, Moretti CL, Jacomino AP
Pesquisa Agropecuária Brasileira 39: 1027-1032, 2004. IF = 0.167
P207-04 Criptosporidiose em camundongos imunodeficientes: dinâmica da eliminação de oocistos de Criptosporidium sp. em linhagens de camundongos imunodeficientes
Alves DP, Britto MHSS, Guaraldo AMA*
Biotecnologia, Ciência & Desenvolvimento 32: 54-60, 2004.
P208-04 Malária em camundongos imunodeficientes: a malária experimental por Plasmodium chabaudi chabaudi em camundongo SCID
Motta FMB, Guaraldo AMA*
Biotecnologia, Ciência & Desenvolvimento 32: 64-68, 2004.
P209-04 Effects of hipocampal lesions on a food location task in pigeons
Ferrari EAM*, Amaral-Thoma M
Behavioural Brain Research 148: 21-34, 2004. IF = 2.992
P210-04 Differential fruit-consumption of two Melatomataceae by birds in Serra da Mantiqueira, southeastern Brazil
Gridipapp CO, Gridipapp M, Silva WR*
Ararajuba 12: 7-13, 2004.
P211-04 Thrichomys apereoides
Reis SF*, Pessoa LM
Mammalian Species 741: 1-5, 2004.
P212-04 Analysis of variance for binary data in unbalanced designs
Souza R, Pinheiro HP, Silva SQ, Reis SF*
Brazilian Journal of Probability and Statistics 18: 129-149, 2004.
P213-04 Phylogeny and circumscription of the near-endemic Brazilian tribe Microlicieae (Melastomataceae)
Fritsch PW, Almeda F, Renner SS, Martins AB*, Cruz BC
American Journal of Botany 91: 1105-1114, 2004. IF = 2.438
P214-04 Proton dependence of tobacco mosaic virus dissociation by presure
Santos JLR, Bispo JAC, Landini GF, Bonafé CFS*
Biophysical Chemistry 111: 53-61, 2004. IF = 2.102
P215-04 Fruit and seed ontogeny related to the seed behaviour of two tropical species of Caesalpinia (Leguminosae)
Teixeira SP, Carmello-Guerreiro SM*, Machado SR
Botanical Journal of the Linnean Society 146: 57-70, 2004. IF = 1.510
P216-04 Biological shape analysis by digital curvature
Costa LD, Reis SF*, Arantes RAT, Alves ACR, Mutinari GC
Pattern Recognition 37: 515-524, 2004. IF = 2.176
P217-04 Morphometrical data analysis using wavelets
Takemura CM, Cesar RM, Arantes RAT, Costa LD, Hingst-Zaher E, Bonato V, Reis SF*
Real-Time Imaging 10: 239-250, 2004. IF = 0.455
P218-04 Larval predation on different instars in blowfly populations
Faria LD, Godoy WAC, Reis SF*
Brazilian Archives of Biology and Technology 47: 887-894, 2004. IF = 0.143
P219-04 Mitotic index of epithelial cells in gills of guppy (Poecilia vivipara) exposed to fractions of the leaf and bark of pequi (Caryocar brasiliensis)
Motter MDS, Silva lD, Oliveira RB, Yamada AT*, Santos SC, Sabóia-Morais SMT
Brazilian Journal of Veterinary Research and Animal Science 41: 220-227, 2004.
P220-04 Effects of natrium fluoride in gill epithelium of guppy fish (Poecilia vivipara)
Breseghelo L, + 6 authors + Yamada AT*
Brazilian Journal of Veterinary Research and Animal Science 41: 274-280, 2004.
P221-04 Advances in bioacoustics
Anais da Academia Brasileira de Ciências 76: i-iv, 2004. IF = 0.435
P222-04 Are communication activities shaped by environmental constraints in reverberating and absorbing forest habitats?
Mathevon N, Aubin T, Dabelsteen T, Vielliard JME*
Anais da Academia Brasileira de Ciências 76: 259-263, 2004. IF = 0.435
P223-04 Complex communication signals: the case of the blue-back grassquit Volatinia jacarina (Alves, Emberizidae) song. Part I – A structural analysis
Fandino-Marino H, Vielliard JME*
Anais da Academia Brasileira de Ciências 76: 325-334, 2004. IF = 0.435
P224-04 How a simple and stereotyped acoustic signal transmits individual information: the song of the white-browed Warbler Basileuterus leucoblepharus
Aubin T, Mathevon N, da Silva ML, Vielliard JME*, Sebe F
Anais da Academia Brasileira de Ciências 76: 335-344, 2004. IF = 0.435
P225-04 Comparative analysis of the song of the rufous-collared sparrow Zonotrichia capensis (Emberizidae) between Campinas and Botucatu, São Paulo, Brazil
Avelino MF, Vielliard JME*
Anais da Academia Brasileira de Ciências 76: 345-349, 2004. IF = 0.435
P226-04 The song of the Brazilian population of Humpback whale Megaptera novaeangilae, in the year 2000: individual song variations and possible implications
Arraut EM, Vielliard JME*
Anais da Academia Brasileira de Ciências 76: 373-380, 2004. IF = 0.435
P227-04 A revision of the South American genus Zollernia Wied-Neuw- & Nees (Leguminosae, Paapilionoideae, Swartzieae)
Mansano VF, Tozzi AMG*, Lewis GP
Kew Bulletin 59: 497-520, 2004.
P228-04 Leguminosae-Papilionoideae no Parque Estadual do Rio Doce, Minas Gerais, Brasil. II. Árvores e arbustos escandentes
Bortoluzzi RLC, Carvalho Okano RM, Garcia FCP, Tozzi AMGA*
Acta Botanica Brasílica 18: 49-71, 2004.
P229-04 Revisão taxonômica de Myrocarpus allemão (Leguminosae, Papilionoideae, Sophoreae)
Sartori ALB, Tozzi AMGA*
Acta Botanica Brasílica 18: 521-535, 2004.
P230-04 Swartzia (Leguminosae, Papilionoideae, Swartzieae s.I.) na reserva natural da Companhia Vale do Rio Doce, Linhares, ES, Brasil
Mansano VF, Tozzi AMGA*
Rodriguesia 55: 95-113, 2004.
P231-04 Flavonoids from Louchocarpus muehlbergianus – Leguminosae
Magalhães AF, Tozzi AMGA*, Magalhães EG, Blanco IS, Soriano MPC
Anais da Academia Brasileira de Ciências 76: 651-661, 2004. IF = 0.435
P232-04 Pollinarium morphology and floral rewards in Brazilian Maxillarinae (Orchidaceae)
Singer RB*, Koehler S
Annals of Botany 93: 39-51, 2004. IF = 2.262
P233-04 Behavioral repertory of the neotropical harvestman Ilhaia cuspidata (Opilliones, Gonyleptidae)
Pereira W, Elpino-Campos A, Del-Claro K, Machado G*
Journal of Arachnology 32: 22-30, 2004. IF = 0.706
P234-04 Five new cases of paternal care in harvestmen (Arachnida: opilliones): implications for the evolution of male guarding in the neotropical family Gonyleptidae
Machado G*, Requena GS, Buzato BA, Osses F, Rossetto LM
Sociobiology 44: 577-598, 2004. IF = 0.578
P235-04 Induced biotic responses to herbivory and associated cues in the Amazonian ant-plant Maieta poeppigii
Christianini AV, Machado G*
Entomologia experimentalis et applicata 112: 81-88, 2004. IF = 0.925
P236-04 Description and ecology of a new species of sun spider (Arachnida: Solifugae) from the Brazilian cerrado
Martins EG, Bonato V, Machado G*, Pinto-da-Rocha R, Rocha LS
Journal of Natural History 38: 2361-2375, 2004. IF = 0.514
P237-04 Observations on the succession patterns of necrophagous insects on a pig carcass in an urban area of southeastern Brazil
Carvalho LML, Thyssen PJ, Goff ML, Linhares AX*
Aggrwal’s Internet Journal of Forensic Medicine and Toxicology 5: 33-39, 2004.
P238-04 Reproductive biology in species of Bidens L. (Asteraceae)
Grombone-Guaratini MT, Solferini VN, Semir J*
Scientia Agrícola 61: 185-189, 2004.
P239-04 Glucocorticoid receptor and beta-adrenoceptor expression in epididymal adipose tissue from stressed rats
Farias-Silva E, dos Santos IN, do Amaral MEC, Grassi-Kassisse DM, Spadari-Bratfisch RC*
Annals of the New York Academy of Sciences 1018: 328-332, 2004. IF = 1.789
P240-04 Microsite specialization and spatial distribution of Geonoma brevispatha, a clonal palm in south-easten Brazil
Souza AF, Martins FR*
Ecological Research 19: 521-532, 2004. IF = 1.037
P241-04 Population structure and dynamics of a neotropical palm in fire-impacted fragments of the Brazilian Atlantic Forest
Souza AF, Martins FR*
Biodiversity and Conservation 13: 1611-1632, 2004. IF = 1.197
P242-04 Reproductive phenology of the cerrado plant community in Emas National Park (central Brazil)
Batalha MA, Martins FR*
Australian Journal of Botany 52: 149-161, 2004. IF = 0.893
P243-04 Floristic, frequency and vegetation life-form spectra of a cerrado site
Batalha MA, Martins FR*
Brazilian Journal of Biology 64: 203-209, 2004.
P244-04 Estrutura do cerradão e da transição entre cerradão e floresta paludícola num fragmento da International Paper do Brasil Ltda., em Brotas, SP
Gomes BZ, Martins FR*
Revista brasileira de Botânica 27: 249-262, 2004.
do Instituto de Biologia
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